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Secreted cellular prion protein binds doxorubicin and correlates with anthracycline resistance in breast cancer

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Figure

Figure 1. PrPC expression in breast tumors and cell lines. (A) Analysis of PRNP mRNA expression in the Metabric data set
Figure 2. Soluble PrPCas the best models of soluble PrPafter brefeldin A treatment (yellow; from Figure 1, G and H), levels of PrPparallel cultures of PRNPsi or SCRsi transfectants
Figure 3. Effect of PrPC-positive serum and tumor tissue on doxorubicin efficacy. (trations (gray shading, 10–100 nM, 24–96 hours after infusion of 75 mg/mof input blotted for HSP70 as a loading control), and the bar graph shows the effect of depleting PrP
Figure 4. Relationships between tumor PrPCsentative immunohistochemical (IHC) detection with SAF32 and 3F4, illustrating the typical staining patterns observed: both plasma membrane and punctate cytoplasmic staining with SAF32; stronger punctate cytoplasmi
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