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Development of a real time reverse transcription loop mediated isothermal amplification method for the rapid detection of porcine epidemic diarrhea virus

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Academic year: 2020

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Figure

Fig. 1 Primer set selection for the real-time RT-LAMP assay. LAMP products were detected by a real-time turbidity assay using an LA-320c (a) anda fluorescence assay (b)
Fig. 2 Optimization of temperature for the real-time RT-LAMP assay. The same reaction mixtures were incubated at 60, 61, 62, 63, 64, or 65 °C for1 h, respectively
Fig. 3 Comparative sensitivity of real-time RT-LAMP, one-step RT-PCR, and real-time RT-PCR methods
Table 1 Detection of PEDV in clinical samples by real-timeRT-LAMP, real-time RT-PCR, and one-step RT-PCR
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