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A one step real time RT PCR assay for the quantitation of Wheat yellow mosaic virus(WYMV)

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Academic year: 2020

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Figure

Figure 1 Optimization concentration of primer and probe: A: standard sample of WYMV as template
Table 1 Detection of WYMV in tenfold serial dilutions of the crude sap from infected wheat by indirectELISA and real-time RT-PCR
Table 2 Detection of WYMV from wheat samples by real-time RT-PCR and gel-based RT-PCR
Figure 3 Determination of relative expression of WYMV using wheat β-actin gene and 18S rRNA as internal reference, and by absolutequantification with cloned standards (absolute).
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