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A sensitive luminescence syncytium induction assay (LuSIA) based on a reporter plasmid containing a mutation in the glucocorticoid response element in the long terminal repeat U3 region of bovine leukemia virus

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Academic year: 2020

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Figure

Fig. 1 Construction of reporter plasmids. Schematic presentation of reporter plasmids bearing several BLV-LTR promoters: the full-length BLV-LTRpromoter; pBLU3-EGFP with the BLV-LTR U3 region promoter; and pBLU3GREM-EGFP with a BLV-LTR U3 region containing
Fig. 2 Comparison of quantitative analysis of cell-to-cell infection by LuSIAs using CC81-GREMG and CC81-BLU3G reporter cells
Fig. 3 Quantitative analysis of cell-free infection by LuSIA using CC81-GREMG.supernatant, as determined by BLV p24 capture, and the number of syncytia expressing EGFP when CC81-GREMG cells were cultured with seriallycollected from FLK-BLV cells, which are
Fig. 4 Use of LuSIA based on CC81-GREMG cells to detect other syncytium-inducible bovine viruses.representative of three independent experiments
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