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P2Y<sub>12 </sub>and P2Y<sub>13</sub> receptors involved in ADP&beta;s induced the release of IL-1&beta;, IL-6 and TNF-&alpha; from cultured dorsal horn microglia

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Figure

Figure 1 Micrographs of cultured dorsal spinal cord microglia cells (Notes:corresponding views of microglia cells labeled for Iba-1 (A–F)
Figure 2 Real-time fluorescence quantitative polymerase chain reaction results show the expression of Iba-1 mRNA in cultured rat dorsal spinal cord microglia cells.(significantly inhibited after pretreatment with MRS2395 (1, 10 and 50 μM) for 20 min
Figure 3 Real-time fluorescence quantitative polymerase chain reaction results show the expression of IL-1does not induce increased expression of IL-10 mRNA in microglia cells
Figure 4 Enzyme-linked immunosorbent assay results show the IL-1ADPNotes:collected, and the amount of IL-1MRS2395 (**b, IL-6, IL-10, and TNF-a release from cultured rat dorsal spinal cord microglia cells
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