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A Zn finger protein, Xfin, is expressed during cone differentiation in the retina of the frog Xenopus laevis

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Academic year: 2020

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Figure

Fig.1. Immunostaininganalysisof retinalXenopusswimmingtad-polesections.stainedwiththeanti-Xfinantibody.(AI The arrowspointto the stainedcones.(BI EnlargementofpanelA.Cos: coneouter segment;ros: rodoutersegment;nu: nucleus,fp: footpiece.IC) Negativecontrolmadeby using preimmuneserum.ONL: outernuclearlayer; OPL: outer plexiformlayer; INL: inner nuclearlayer; IPL: inner plexiformlayer; GCL: ganglioncel!layer.Bars represent5 11m.
Fig.2. Westernanti-Xfln2 serum. raised against a region of Xfin not containing fingersand with the anti-Xfin serumblot analysis of Xenopusadulteye extracts.Proteinswere separatedon a 10% 50Spolyacrylamidegel and stainedwith the la) Ib)
Fig. 4. Immunostaininganalysis of retinal stage X. (S) Enlargement of (AI. The nomenclature of the retinallayers is as in Fig 1
Fig. 6. Immunostaininganalvsisof aretinalBufotadpolesection,show-ing the sameantigenicitypatternasinXenopus.Nomenclatureoftheretinal layers as In Fig

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