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“A Review on Implementation of Bacillus Bacteria for Restoration of

Damaged Concrete”

Akash Adinath Kadam

1

, Manisha Jamgade

2

1Post Graduate Student in Civil Engineering, Pillai HOC College of Engineering and Technology, Rasayani,

Dist.Raigad-410207, Maharashtra, India

2Associate Professor in Civil Engineering Department, Pillai HOC College of Engineering and Technology, Rasayani,

Dist.Raigad-410207, Maharashtra, India

---***---Abstract: Concrete use is growing rapidly across the

globe and thus the production of concrete is useful for environment bases also. Concrete is prepared in very less time when it comes into water contact, Autonomous cure for concrete cracks. In this work we examined the bacteria's capacity to act as a concrete self-healing agent, i.e. their ability to patch cracks that occur. For this reason, a particular group of concrete is very useful for construction purposes. Bacterial spores applied directly to the mixture of cement pastes are used on a monthly basis. A persistent low in the diameter of the pore during cement stone setting possibly restricted the spore lifespan, the typical size of Bacillus spores. Nevertheless, because Bacterial cement stone cases appeared to develop considerably more crack-plugging minerals than control specimens, and the possible use of bacterial spores as a self-healing agent seems encouraging. This work is about concrete performance with bacterial concrete and how it improves its mechanical properties.

Keywords: - Concrete, Bacillus Bacteria, Self-Healing

Concrete

1. INTRODUCTION

The greatest recognized species of the Bacillus, B. Megaterium is roughly 1.5 μm long (micrometers; 1 μm = 10−6 m). Bacillus also occurs within chains. In 1877 the German Scientist gave an Authoritative description of two forms of hay bacillus (now called iBacillus isubtilis): One that could be destroyed during heat exposure, and one that was heat resistant. He named "spores" (endospores) under adverse environmental conditions All Bacillus species can produce lethargic spores. These endospores will continue to be reasonable for extended periods of time.

Endospores are heat, synthetic concoctions, and day light safe, and generally circulate in nature, mainly in soil from which residue particles are attacked.

Cement (Portland clinker) Development alone is expected to contribute 7% to the world's anthropogenic CO2 emissions, owing in particular to the sintering to calcareous and clay at temperature 1500 ° C, as calcium carbonate (CaCO3) is converted to calcium oxide (CaO) during this process while releasing CO2. Nonetheless concrete does not seem to be a safe substance from an environmental point of view (Gerilla et al., 2007).

2. HISTORY

The idea of self-healing concrete was first developed in the early 1990s. The concept branched from the idea that concrete without admixtures or biological healing agents is capable of healing itself. This process occurs very slowly and not to the extent needed to recover water-tightness. Researchers began testing ways to speed up the process. The first to seriously consider the idea. She proposed including glass capsules in the concrete that contained methyl methacrylate glue that would be released when the capsules were broken. However, the glue was too viscous to flow fast enough to fill cracks and the glass capsules would not survive the concrete mixing process. Others have tried healing mediums in concrete such as polymers, gels, clays, waxes, and films to varying degrees of success. In the mid-2000s, Jonkers and Schlangen began to research encapsulated bacteria as a biological healing agent. All these proposed methods had the same idea, as described by Patel: “The trick to making self-repairing concrete is to heal microscopic fissures before they become large cracks”. After success in the lab, Jonkers

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and Schlangen have constructed the world’s first building of their bacterial self-healing concrete that operates by the mechanism Patel describes. This building provides them with important data on how the material performs in real-world conditions.

3. BACTERIAL SELF-HEALING CONCRETE

One material that is resistant to the inevitable cracks is bacterial self-healing concrete. This technology utilizes dormant bacteria sealed in capsules in the concrete matrix. As described by Jonkers and Schlangen,. The water ingress activates the bacteria to Calcium carbonate which is produced (also known as calcite or limestone) which fills the crack. By filling the crack quickly, this concrete prevents larger cracks from forming and stops further oxidation of the steel supports.

4. LITERATURE REVIEW

Nain et al. (2019) Adopted the problem of micro-crack management and also checked the improvement of compressive and tensile strength of concrete by different microbes. There are many integrations used in concrete. The blocks were then cast, cured for 7 and 28 days, and compressibility tested, along with tensile strength testing was carried out. [1]

Putri et al. (2019) Examined the applicability to small-scale concrete unique specimens of bio-based repair materials. In order to determine the effectiveness of the selected blends for sealing cracks in concrete specimens, water permeability tests were performed on this study.[2]

Saradha et al. (2019) Presented concrete results through the unique growth induced microbiologically. This has led to the creation of a very special concrete, or otherwise called this type of concrete known as self-healing, which induces bacteria to heal faults in mortars and concrete. Researchers with various bacteria proposed specific concrete. Here the bacteria "bacillus subtitles'' is tried.[3]

Munyao et al. (2019) The probability of MICP biodeposition as an obstacle to the entry of sulphate particles in a sulphate-rich environment in microbial

remunerated concrete. The effect of material and physical sulfate assailment is referred to as the properties of mechanical are used as the compressive consistency, whereas the smallest measurement depends on the electron microscopy assessing.[4]

VijayaSundravel et al. (2019) Bacillus Bacteria strength behavior and enhancing features of GFRP sheets are studied. In this analysis, a compressive strength test indicates the optimum percentage of Bacillus bacteria. In the course of the axial compression test, strengthening columns are tested. This results in the calculation of the ultimate column load capacity, deflection, stiffness and energy absorption.[5]

Sudha et al. (2019) the healing of artificial cracks in the concrete by introducing bacteria different proportions such as 10ml, 20ml, 30 ml,40ml and the effect of compressive strength, flexural strength and split tensile test due to mixing in concrete is also discussed in this paper. The Bacteria which is going to be introduced should be alkali resistant against the stresses while mixing, transporting and placing. It is also noted that bacillus cereus arrest the cracks in the concrete and acts as an excellent self-healing agent[6]

Xu et al. (2018) The combined pH , temperature, and urea dosage were the main factors contributing to the MICP biochemical process. The urea dosage has a major effect on urea degradation and CaCO3 precipitation levels under a pleasant pH and temperature setting.[7]

Seifan et al. (2018) The biotechnological method is characterized by the induction of the most compatible material with concrete composition, as investigated self-healing strategies. This cycle integrates the strong bacteria and nutrients in the concrete matrix[8]

Basha et al. (2018) Describing the comprehensive experimental study on cement mortar compressive strength, mixed with six alkali-philic bacteria isolated from high-alkalinity surface mica mines.[9]

Gautamet al. (2018) A two-component healing agent, consisting of bacteria and a mineral precursor compound, was applied to the concrete mix. When the device is broken, the intake water is disabled. The

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mineral precursor compound is converted into the mineral calcium carbonate known as the calcareous calcium. Calcular precipitation on the crack surface allows for cracks to be sealed and plugged and makes the matrix less accessible for water and other deleterious substances.[10]

Sharma et al. (2017) The characterization of alkaliphilic species Bacillus are used in concrete for concrete micro crack remediation was submitted for calcium produce, germination and calcite formation. Conditions, extent of production and timing of fine spore have been determined by light microscopy of dark-fields; germination and kinetics have been evaluated by combining optical density reduction with phase contrast microscopic formation of refractile bodies.[11]

Kalhori et al. (2017) Bacillus Subtilis offered an evaluation of the impact of healing and mechanical shotcrete properties. In order to test the effect of each method on there are many tests used like tensile test, compressive test, etc. and bacteria have been added with the mixed design and curing solution.[12]

Zhang et al. (2017) In a quantification of the cracks in concrete by immobilisation of Bacillus cohnii, it was demonstrated the viability of expanded perlite (EP) as a new bacteria carrier. Effects on the performance of crack-healing were also studied by two other self-healing methods, that is, by the direct injection of bacteria and expanded clay (EC). [13]

Joshi et al. (2017) The ability of concrete structure crack repair on the basis of bacteria, and the use of various bacterial therapies for self-healing cracks, has become a major focus. In addition, guidelines on the commercial use of MICCP technology and cost reduction are issued [14].

Zamani et al. (2017) Presented as promising technologies for the bioremediation of these structures the description of microbial induced carbonate precipitations. At first they were transferred to the laboratory with the help of swaps in the preservation of stone monuments in nutrient broth at the historical cemetery. Gram-positive bacilli were detected after incubation and colonial growth. [15]

Richardson et al. (2016) Micro-induced calcite precipitation for better concrete finishing, screening cracks and the cementing of loose surface particles together provided sealing and healing properties. Sporosarcina pasteurii was used to precipitate calcium carbonate effectively for the processing of porous material. The tests conducted assessed the impact and surface consolidation of the MICP on samples of 24 concrete cubes.[16]

Tziviloglouet al. (2016) In order to protect existing structures by using more concrete-incompatible and environmentally friendly materials than current reparations materials, positive results of laboratory experiments were demonstrated in all these groundbreaking concepts. The first full-format outdoor applications have been taken to illustrate the versatility of this new technology.[17]

Suthar et al. (2016) Presented microorganism behavior Bacillus Subtilis for strength enhancement of broken concrete specimens. In practical words, crack is a very significant phenomenon due to low tensile strength and stress that induces concrete settlement, shrinkage and expansion.[18]

5. THE IMPACT OF SELF-HEALING CONCRETE 5.1 Economic Considerations

As detailed above, the current method of dealing with concrete’s natural tendency to form cracks involves the economically unsustainable methods of individual crack treatment and over-dimensioning. The inherently high costs of these solutions and their temporary nature suggest the need for a solution that is more economically feasible in the long run.

This is one of the major reasons why the material is not currently being mass produced. According to Bravo Silva, “The bio-based additive for concrete, consisting of encapsulated spores to mix in the concrete before the casting process, results in prices of €5760 ($6522) per m3”. Considering concrete itself is rather inexpensive to produce, any product applied to concrete. It will take significant research to reduce the upfront costs of self-healing concrete before

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contractors will be convinced of its superiority to traditional concrete and implement it on a wider scale Part of the reason that bacterial self-healing concrete has such a high initial cost is because it is difficult to prepare the bacteria and the capsules that contain them. The microbes themselves require completely aseptic conditions, pricey growth mediums, and extensive labor to produce. Additionally, the successful encapsulation of the bacteria is variable and contributes €30 to € 50 ($34 to $57) per kilogram of bacteria produced to the overall cost of the material. The calcium lactate that the bacteria require as a food source is also expensive, but Jonkers and Schlangen are researching a cheaper sugar-based nutrient that could replace it. The bacterial production process and the medium of encapsulation are two areas where future research could lower production cost.

6. Discussion

6.1 Self-Healing Mechanism

Biological and self-healing concrete, or MICP, creates CaCO3 by use of bacteria. This fills out gaps found in the concrete. Bacillus subtilis, Bacillus pseudofirmus, Bacillus pasteurii, the effect is as a by-product of calcium carbonate. Many reactions also increase the pH, producing bicarbonate and carbonate ions, from acidic to alkaline conditions. These precipitate into the concrete with the calcium ions to form the minerals of calcium carbonate.

7. CONCLUSION

The incorporation of bacteria into the concrete makes it very efficient, improving the concrete quality that is more than normal concrete. Bacteria repair concrete cracks by producing the Calcium Carbonate crystal which blocks and repairs the cracks. Calcium carbonate crystal production Diminishes water permeability by reducing crack width. Due to the use of self-healing material used in concrete, frequent inspection of the concrete would be less required. Concrete life should be strong as opposed to traditional concrete. This has a high longevity and strength as opposed to traditional concrete.

8. REFERENCES

1. Nain, Nidhi, R. Surabhi, N. V. Yathish, V. Krishnamurthy, T. Deepa, and Seema Tharannum. "Enhancement in strength parameters of concrete by application of Bacillus bacteria." Construction and Building Materials 202 (2019): 904-908. 2. Putri, Prima Yane, Isao Ujike, and Keiyu Kawaai.

"Application of bio-based material for concrete repair: case study leakage on parallel concrete slab." In MATEC Web of Conferences, vol. 258, p. 01013. EDP Sciences, 2019.

3. Saradha, P., K. Vidhya, and S. Visali. "Experimental Investigation on Bacterial Concrete Using Bacillus Subtitles." International Research Journal of Multidisciplinary Technovation 1, no. 6 (2019): 207-219.

4. Munyao, Onesmus Mulwa, Joseph Karanja Thiong'o, Jackson Muthengia Wachira, Daniel Karanja Mutitu, Mwirichia Romano, and Genson Murithi. "Use of Bacillus Species bacteria in protecting the concrete structures from sulphate attack-a review." Journal of Chemical Reviews 1, no. 4, pp. 252-299 (2019): 287-299.

5. VijayaSundravel, K., S. Ramesh, and D. Jegatheeswaran. "Strengthening of RC Short Column with Partial Replacement of Bacillus Bacteriain Cement." International Research Journal of Multidisciplinary Technovation 1, no. 6 (2019): 362-372.

6. Sudha, N., T. Gowsalaya, A. Hemavarshini, and S. Gokula Lakshmi. "Experimental Investigations on Strength Properties of Self-healing Bacterial Concrete Using M-Sand." International Journal 6, no. 4 (2019): 1-4.

7. Xu, Jing, Xianzhi Wang, and Binbin Wang. "Biochemical process of ureolysis-based microbial CaCO 3 precipitation and its application in self-healing concrete." Applied microbiology and biotechnology 102, no. 7 (2018): 3121-3132. 8. Seifan, Mostafa, Alireza Ebrahiminezhad, Younes

Ghasemi, Ali Khajeh Samani, and Aydin Berenjian. "Amine-modified magnetic iron oxide nanoparticle as a promising carrier for application in bio self-healing concrete." Applied microbiology and biotechnology 102, no. 1 (2018): 175-184.

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9. Basha, Sreenivasulu, Lakshman Kumar Lingamgunta, Jayakumar Kannali, Swarna Kumari Gajula, Ramesh Bandikari, Sreenivasulu Dasari, Veena Dalavai et al. "Subsurface endospore-forming bacteria possess bio-sealant properties." Scientific reports 8, no. 1 (2018): 1-13. 10. Gautam, B. R. "Bacteria based self-healing concrete–a bacterial approach." International Journal of Engineering and Science (2018): 57-61. 11. Sharma, T. K., Mohamed Alazhari, Andrew Heath, Kevin Paine, and R. M. Cooper. "Alkaliphilic Bacillus species show potential application in concrete crack repair by virtue of rapid spore production and germination then extracellular calcite formation." Journal of applied microbiology 122, no. 5 (2017): 1233-1244.

12. Kalhori, Hamid, and Raheb Bagherpour. "Application of carbonate precipitating bacteria for improving properties and repairing cracks of shotcrete." Construction and Building Materials 148 (2017): 249-260.

13. Zhang, Jiaguang, Yuanzhen Liu, Tao Feng, Mengjun Zhou, Lin Zhao, Aijuan Zhou, and Zhu Li. "Immobilizing bacteria in expanded perlite for the crack self-healing in concrete." Construction and Building Materials 148 (2017): 610-617.

14. Joshi, Sumit, Shweta Goyal, Abhijit Mukherjee, and M. Sudhakara Reddy. "Microbial healing of cracks in concrete: a review." Journal of industrial microbiology & biotechnology 44, no. 11 (2017): 1511-1525.

15. Zamani, Narges, Mohsen Ghezelsofla, Ali Mohammad Ahadi, and Marziye Zamani. "Application of Microbial Biotechnology in Conservation and Restoration of Stone Monument." Journal of Applied Biotechnology Reports 4, no. 2 (2017): 587-592.

16. Richardson, Alan, Kathryn Coventry, and Jack Pasley. "Bacterial crack sealing and surface finish application to concrete." (2016): 1716-1724. 17. Tziviloglou, Eirini, Kim Van Tittelboom, Damian

Palin, Jianyun Wang, M. Guadalupe Sierra-Beltrán, Yusuf Çagatay Erşan, Renée Mors et al. "Bio-based self-healing concrete: from research to field application." In Self-healing Materials, pp. 345-385. Springer, Cham, 2016.

18. Suthar, G. T., and K. B. Parikh. "A Study of Microorganism (Bacteria) On Concrete Strength and Durability." International Journal For Technological Research In Engineering 3, no. 12 (2016).

19. Wu, Mingyue, Xiangming Hu, Qian Zhang, Di Xue, and Yanyun Zhao. "Growth environment optimization for inducing bacterial mineralization and its application in concrete healing." Construction and Building Materials 209 (2019): 631-643

References

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