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Gene trapping: An antibody-dependent approach for verifying integration in your favorite gene

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Figure

Fig. 1. DCLK2 antibodies immunoprecipitate myc-tagged DCLK2. HEK293 cells were  transfected with myc-tagged DCLK2 and immunoprecipitated with preimmune serum or  DCLK2 antibodies
Fig. 2. The schematic organization of Dclk2. The gene is composed of 16 exons (5’ and 3’  UTR are shown in black, and the translated exons are in grey)
Fig. 4. The LacZ reaction of immunoprecipitated proteins. Cell lysates from AZ0780,  AC0704 and Z/EG/DCX were immunoprecipitated with DCLK2 antibodies (top row) or  preimmune serum (bottom row)
Fig. 6. The LacZ reaction of the immunoprecipitated proteins. The cell lysate from  AZ0780 was diluted to different concentrations and immunoprecipitated with DCLK2  antibodies (top row) or preimmune serum (bottom row)

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