Scientific Method
Scientific Theory
A
theory
is an explanation of a set of related
observations or events, supported by proven
experiments, and verified multiple times by
many independent scientists.
How Does a Theory
differ from a Law?
Observation vs.
Inference
•
Observation:
Observation vs.
Inference
•
Inference:
Steps in the Scientific
Steps in the Scientific
Method
Method
Observation
Observation
State the
State the
Problem
Problem
Hypothesis
Hypothesis
Experiment
Experiment
Data Collection
Data Collection
Conclusion
Conclusion
Observation
How would you explain what you see?
Plants grow differently in differently colored
1. State the
Problem
What problem are you trying to solve?
Always write it as a
question
beginning with
DOES
or
WILL
….?
Ex. Does red light affect the growth of a
2. State Your Hypothesis
Hypothesis = an
educated guess
.
Written as --- If….then
statement
.
Hypothesis must be
testable
.
Ex. If the red light is used, then
Example situations –
Using Temperature
and Breathing Rate
Here are some
possible
hypotheses.
Hypothesis:
If
the water temperature is increased,
Formulate a hypothesis on
the following topics:
1. Smoking
2. Global
Warming
3. Steroids
Set up a
Controlled Experiment
There are always
two groups
in an experiment:
•
Experimental Group
– receives the treatment, the
“variable”
•A factor in the experiment that is being
changed
•The
independent
variable
is the one factor that
is
being
changed by
experimenter
What is the
dependent variable
then?
The variable that is measured
in the experiment.
Ex. --- growth of a plant, breathing rate of the fish
All of the other
conditions that are kept the same
between the two groups.
Let’s Practice
Control group:
Experimental Group
:
Independent variable:
Dependent variable:
Constants:
Problem
: Does the water temperature
affect the breathing rate of the fish?
4. C
ollect Data
•Utilize a
chart, table or graph
to
organize data.
Graphing Basics
Independent Variable: - the cause. (the “if” of if, then)
always plotted on the x-axis
- factor in the experiment that the experimenter changes
-you know the values before you begin the experiment
Dependent Variable:- the effect (the “then” of if, then)
always plotted on the y-axis
- factor that is measured as the experiment proceeds
pH
pH
Plant Growth
Plant Growth
after 1 hour
after 1 hour
Plant Growth
after 2 hours
Plant Growth
after 2 hours
Plant Growth
after 3 hours
Plant Growth
after 3 hours
2
2
10 cm.
10 cm.
15 cm.
15 cm.
22 cm.
22 cm.
4
4
6 cm.
6 cm.
12 cm.
12 cm.
15 cm.
15 cm.
6
6
5 cm.
5 cm.
10 cm.
10 cm.
12 cm.
12 cm.
8
8
4 cm.
4 cm.
9 cm.
9 cm.
10 cm.
10 cm.
10
A scientist is conducting an experiment to determine the
number of oxygen bubbles produced by plants at
varying levels below the surface of water.
What is the independent variable?
What is the dependent variable?
What is the title of the graph?
What will you label the x-axis?
5. Draw A
Conclusion
•Draw a conclusion
based on the data
.
(you should
reference your data, use #’s)
•Determine if your hypothesis was
Repeated trials
- do the
experiment many times for
valid results.
Large sample size
– use
many subjects for valid
results.
•
Too Few Subjects:
not a large
enough sampling to draw a valid
conclusion from.
Question # 1
A new concept that is tested in a scientific
investigation is known as
a.
A theory
b.
The hypothesis
c.
An inference
Question # 2
Which procedure must be followed for
the results of an experiment to be
considered valid?
a.
The experiment must be repeated a number
of times and yield similar results.
b.
After one trial, the results of the experiment
must be published.
c.
The results must be expressed in the form of
a graph or table.
Question # 3
An investigation was designed to determine
the effect of sunlight on mold growth. Two
groups of mold were grown under identical
conditions except that one group was
exposed only to sunlight, while the other was
grown in total darkness. In this
investigation, the group of mold grown
without receiving sunlight is known as the
a. Experimental variable b. Dependent variable c. Control
Question # 4
A biologist in a laboratory reports a new
discovery based on experimental results. If the
results are valid, biologists in other laboratories
should be able to
a. Repeat the same experiment with a different variable and get the same results.
b. Perform the same experiment and obtain different results.
c. Repeat the same experiment and obtain the same results.
d. Perform the same experiment under different
Question # 5
To test the effect of hormones (auxins) on plant growth,
6 plant seedlings of the same species were measured and then sprayed with auxin (growth hormone). After 4 weeks of growth under ideal conditions, the plants were measured again. To set up a proper control for this
experiment, the investigator should
a. Spray the same plants with different amounts of auxin.
b. Spray auxin on 6 plant seedlings of the same species and grow them in the dark.
c. Wash the auxin off 3 of the plants after 2 weeks.
d. Grow another 6 plant seedlings of the same species under the same conditions, spraying them with