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Fluorescent tagging of VP22 in N-terminus reveals that VP22 favors Marek’s disease virus (MDV) virulence in chickens and allows morphogenesis study in MD tumor cells

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Academic year: 2020

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Figure

Figure 1 Construction and characterization of rRB-1B EGFP22 in cell culture. A. Schematic representation of the shuttle plasmid constructedto generate the rRB-1B EGFP22 mutant by using homologous recombination in E
Figure 2 MD incidence and tumor formation in chickens infected with the rRB-1B EGFP22 virus
Figure 3 MDV genome copy number in blood cells and feather tips of chickens. The rRB-1B EGFP22 or rRB-1B virus (1500 pfu) was inoculatedintramuscularly in 1 week-old White Leghorn B13/B13 chickens (Experiment 2)
Figure 4 EGFP fluorescent cells in rRB-1B EGFP22-induced tumors and selection by cell-sorting
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