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Iron-Inducible Nuclear Translocation of a Myb3 Transcription Factor in the Protozoan Parasite Trichomonas vaginalis

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Academic year: 2020

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Figure

FIG 1 Overexpression of HA-Myb3 in T. vaginalis. (A) In pAP65-2.1-ha-myb3, an ap65-2.1 promoter drives expression of the HA-tagged myb3 geneand the �-tubulin (TUB) promoter drives the neo gene
FIG 2 The polybasic sequence and Myb3’s nuclear localization. (A) Relativelocations of Myb3= R1R2R3 structural domains (helices), a �-hairpin (twoarrows), and a polybasic (PB) sequence are depicted
FIG 3 Deletion mapping to define Myb3’s NLS. (A) The relative locations of the R1, R2, and R3 domains and a polybasic (PB) sequence in Myb3 are indicatedby numbers
FIG 5 Effects of a conserved isoleucine, I79, on nuclear importation, DNA-binding activity, and the structural integrity of Myb3
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