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Isolation of Recombinant Antibodies against EspA and Intimin of Escherichia coli O157:H7

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Figure

TABLE 1. Primers for scFv phage display library construction
FIG. 1. Purification of recombinant E. coliproteins were expressed and purified as MBP fusions from pMalc2(A) or as His-tagged proteins from pET21b (B)
FIG. 5. Filter screening of antibodies to intimin282. Soluble scFvwere selected for binding to a filter coated with His-tagged intimin282
FIG. 6. Deduced amino acid sequences of representative scFvs against intimin (I1 to I19) and EspA (E1, E4, and E5)
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