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Development of a Novel Nonradioisotopic Assay and Cdc25B Overexpression Cell Lines for Use in Screening for Cdc25B Inhibitors

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Figure

Fig. 1. A nonradioisotopic assay method to measure Cdk1 kinase activity.  We used two kinds of Cdk1 kinases, one was Cdk1 IP and the other was com-mercially available Cdk1
Fig. 2. Optimization of several conditions for the Cdk1 kinase assay. Several key factors were examined to optimize the conditions of the Cdk1 kinase as-say
Fig. 4. Construction and characterization of Cdc25B2 and Cdc25B3 over-expression cell lines
Table 1. Differentially Expressed or PTM Proteins in Cdc25B Overexpression Cell Lines
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