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i GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases

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Academic year: 2020

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Figure

Fig. 1 Evaluation of earlier time points for performing GONAD. a Diagrammatic illustration showing the anatomical structures of ovary and oviduct andthe surgical equipment used for GONAD procedure
Fig. 2 Creating gene-inactivated animal models using the GONAD method. a Schematic of the targeting strategy to inactivate Foxe3 gene andthe primer set used for genotyping
Fig. 3 Creating small genetic change animal models using thefetuses are indicated byindicated in # numbers (shown inresults of PCR products amplified from the G0 fetuses ingene editing
Fig. 4 Creating large deletion using the i-GONAD method. a Schematic diagram showing deletion of 16.2-kb sequence consisting of retrotransposonin the C57BL/6JJcl mouse genome, to restore agouti phenotype
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