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Rescuing lymphocytes from HLA-G immunosuppressive effects mediated by the tumor microenvironment

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Academic year: 2020

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Figure

Figure 1: expression of HlA-G and its ligand on tumor and immune cells. Flow cytometry assessment of ILT2 (HLA-G ligand) on NKL cells A
Figure 2: nK cells can be engineered to silence Ilt2. ILT2representative experiment out of four independent experiments performed
Figure 3: Unimpaired migration activity ILT2-shRNA modified NK cells. NKL (black bars) and NK-10 (ILT2bars) cells were used in transwell assays to assess their migration with or without chemoattractant
Figure 4: silencing Ilt2 restores the proliferation of nK cells. Parental and modified NK cells were co-cultured with tumor cells with or without HLA-G expression (upper panel-A K562, lower panel-B LCL)
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