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RBPJ maintains brain tumor–initiating cells through CDK9 mediated transcriptional elongation

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Figure

Figure 1. NOTCH inhibition does not attenu-ate cell autonomous BTIC growth.ase reporter together with a tk-renilla reporter, and then treated with the NOTCH antagonist, DAPT (5 are displayed as mean ± SEM for the ratio of firefly-to-renilla luciferase (Stu
Figure 2. RBPJ is highly expressed in BTICs. (A) Cleaved NOTCH1 levels were assayed by immunoblot in matched BTICs and non-BTICs isolated from patient-derived xenografts (387, 3691, and 4121)
Figure 3. Targeting RBPJ decreases BTIC growth and self-renewal. (A and B) Effects of shRNA against RBPJ were tested in two BTIC models (3691 and 4121)
Figure 4. Targeting RBPJ decreases BTIC tumor formation. (A) Tumor size of orthotopic glioblastoma xenografts derived from luciferase-expressing BTICs transduced with shCONT or shRBPJ was tracked by bioluminescence over a time course
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