TEST DEFINITION LINEAR MODEL CALIBRATION DEFINITION TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST URICO

Ácido Úrico

Uricasa – POD.

CALIBRACIÓN

Utilizar un suero de calibración adecuado

CONDICIONES OPERATORIAS

Introducir en la memoria del ordenador los siguientes parámetros:

** Introducir el valor asignado al calibrador

LINEALIDAD

La técnica es lineal al menos hasta 25.0 mg/dL de Ácido Úrico.

TEST DEFINITION

ENTRY NAME URI SAMPLE NORMAL 5.00

REPORT NAME URICO LOW 10.00 HIGH 2.50

RATIO REF

TEST NUMBER UNITS PRIM MG/DL SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG END PT PRINT DIGITS 1

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 2.50000 TO 7.50000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME URI TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG END PT

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -1000.00 TO 1000.00 REF CAL FACTOR

% TOL OF CAL FACTOR 10 % TOL OF CAL 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

SPINTROL ** 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST URICO

REAGENT NAME URI LINEARITY (C) 0.00000 TO 10.00000

LOT ID * INITIAL Ad. 0.300

ABS LIMIT (Ad) 2.40

REAGENT VOL (uL) NORM 236 REAGENT BLANK YES

LOW 236 HIGH 286 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 180 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 240 MIX TIME (SEC) 1.00

NUMBER OF READS 1 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

516 / 660 A 1.00 .000 .000

Uric acid

Uricase – POD.

CALIBRATION

Use a human serum calibrator

OPERATORY CONDITIONS

Introduce the following data in the computer :

** Assign the values of controls and calibrator.

LINEARITY

This method is linear up to 25.0 mg/dL of Uric Acid.

TEST DEFINITION

ENTRY NAME URI SAMPLE NORMAL 5.00

REPORT NAME URIC LOW 10.00 HIGH 2.50

RATIO REF

TEST NUMBER UNITS PRIM MG/DL SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG END PT PRINT DIGITS 1

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 2.50000 TO 7.50000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME URI TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG END PT

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -1000.00 TO 1000.00 REF CAL FACTOR

% TOL OF CAL FACTOR 10 % TOL OF CAL 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

SPINTROL ** 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST URIC

REAGENT NAME URI LINEARITY (C) 0.00000 TO 10.00000

LOT ID * INITIAL Ad. .300

ABS LIMIT (Ad) 2.40

REAGENT VOL (uL) NORM 236 REAGENT BLANK YES

LOW 236 HIGH 286 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 180 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 240 MIX TIME (SEC) 1.00

NUMBER OF READS 1 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

516 / 660 A 1.00 .000 .000

Albúmina

Verde bromocresol.

PREPARACIÓN Y ESTABILIDAD

El reactivo esta listo para su uso.

Estable hasta su fecha de caducidad. Proteger de la luz.

CALIBRACIÓN

Utilizar un suero de calibración adecuado

CONDICIONES OPERATORIAS

Introducir en la memoria del ordenador los siguientes parámetros:

** Introducir el valor asignado al calibrador

LINEALIDAD

La técnica es lineal al menos hasta 6.0 g/dL de Albúmina.

TEST DEFINITION

ENTRY NAME ALB SAMPLE NORMAL 1.25

REPORT NAME ALBUMINA LOW 2.50 HIGH 1.25

RATIO REF

TEST NUMBER UNITS PRIM G/DL SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG END PT PRINT DIGITS 1

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 3.50000 TO 5.00000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME ALB TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG END PT

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -2000.00 TO 2000.00 REF CAL FACTOR

% TOL OF CAL FACTOR 10 % TOL OF CAL 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

SPINTROL ** 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST ALBUMINA

REAGENT NAME ALB LINEARITY (C) 0.50000 TO 6.00000

LOT ID * INITIAL Ad. .500

ABS LIMIT (Ad) 2.50

REAGENT VOL (uL) NORM 236 REAGNET BLANK YES

LOW 236 HIGH 286 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 310 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 420 MIX TIME (SEC) 1.00

NUMBER OF READS 1 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

636 / 500 A 1.00 .000 .000

Albumin

Bromocresol green.

PREPARATION AND STABILITY

The reagent is ready to be used.

This solution is stable at 2-8ºC up to the date of expiration specified. Avoid direct sunlight.

CALIBRATION

Use a human serum calibrator

OPERATORY CONDITIONS

Introduce the following data in the computer :

** Assign the values of controls and calibrator.

LINEARITY

This method is linear up to 6.0 g/dL of Albumin.

TEST DEFINITION

ENTRY NAME ALB SAMPLE NORMAL 1.25

REPORT NAME ALBUMIN LOW 2.50 HIGH 1.25

RATIO REF

TEST NUMBER UNITS PRIM G/DL SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG END PT PRINT DIGITS 1

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 3.50000 TO 5.00000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME ALB TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG END PT

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -2000.00 TO 2000.00 REF CAL FACTOR

% TOL OF CAL FACTOR 10 % TOL OF CAL 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

SPINTROL ** 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST ALBUMIN

REAGENT NAME ALB LINEARITY (C) 0.50000 TO 6.00000

LOT ID * INITIAL Ad. .500

ABS LIMIT (Ad) 2.50

REAGENT VOL (uL) NORM 236 REAGNET BLANK YES

LOW 236 HIGH 286 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 310 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 420 MIX TIME (SEC) 1.00

NUMBER OF READS 1 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

636 / 500 A 1.00 .000 .000

Fosfatasa Alcalina

DGKC

CALIBRACIÓN

Calibración frente a factor.

CONDICIONES OPERATORIAS

Introducir en la memoria del ordenador los siguientes parámetros:

** Introducir el valor asignado al calibrador

LINEALIDAD

La técnica es lineal al menos hasta 750 U/L de Fosfatasa Alcalina.

TEST DEFINITION

ENTRY NAME ALP SAMPLE NORMAL 5.00

REPORT NAME FOSFATASA ALCALINA LOW 25.00 HIGH 2.50

RATIO REF

TEST NUMBER UNITS PRIM IU/L SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG RATE KIN BLNK PRINT DIGITS 0

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 98.0000 TO 279.000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME ALP TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG RATE KIN BLNK

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -1000.00 TO 1000.00

% TOL OF CAL FACTOR 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST FOSFATASA ALCALINA

REAGENT NAME ALP LINEARITY (C) 5 TO 750

LOT ID * INITIAL Ad. 0.800

ABS LIMIT (Ad) 2.400

REAGENT VOL (uL) NORM 236 REAGENT BLANK NO

LOW 236 HIGH 336 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 60 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 240 MIX TIME (SEC) 1.00

NUMBER OF READS 3 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

412 / 484 A 1.00 18.8 .000 .000

Alkaline Phosphatase

DGKC

CALIBRATION

Calibration with a factor.

OPERATORY CONDITIONS

Introduce the following data in the computer :

LINEARITY

This method is linear up to 1200 U/L of Alkaline phosphatase.

TEST DEFINITION

ENTRY NAME ALP SAMPLE NORMAL 5.00

REPORT NAME ALKALINE PHOSPHATASE LOW 25.00 HIGH 2.50

RATIO REF

TEST NUMBER UNITS PRIM IU/L SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG RATE KIN BLNK PRINT DIGITS 0

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 98.0000 TO 279.000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME ALP TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG RATE KIN BLNK

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -1000.00 TO 1000.00

% TOL OF CAL FACTOR 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST ALKALINE PHOSPHATASE

REAGENT NAME ALP LINEARITY (C) 5 TO 750

LOT ID * INITIAL Ad. 0.800

ABS LIMIT (Ad) 2.400

REAGENT VOL (uL) NORM 236 REAGENT BLANK NO

LOW 236 HIGH 336 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 60 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 240 MIX TIME (SEC) 1.00

NUMBER OF READS 3 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

412 / 484 A 1.00 18.8 .000 .000

α

α

α

α

-Amilasa

CNP-G

3

CALIBRACIÓN

Calibración frente a factor.

CONDICIONES OPERATORIAS

Introducir en la memoria del ordenador los siguientes parámetros:

LINEALIDAD

La técnica es lineal al menos hasta 2000 U/L de Amilasa.

TEST DEFINITION

ENTRY NAME AMI SAMPLE NORMAL 5.00

REPORT NAME AMILASA LOW 15.00 HIGH 3.00

RATIO REF

TEST NUMBER UNITS PRIM IU/L SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG RATE KIN BLNK PRINT DIGITS 0

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 0.00000 TO 90.00000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME AMI TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG RATE KIN BLNK

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -2000.00 TO 2000.00 REF CAL FACTOR

% TOL OF CAL FACTOR 10 % TOL OF CAL 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST AMILASA

REAGENT NAME AMI LINEARITY (C) 1.50000 TO 2000.0000

LOT ID * INITIAL Ad. 1.000

ABS LIMIT (Ad) 2.40

REAGENT VOL (uL) NORM 236 REAGENT BLANK NO

LOW 236 HIGH 336 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 60 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 180 MIX TIME (SEC) 1.00

NUMBER OF READS 3 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

404 / 660 A 1.00 8.00 .000 .000

α

α

α

α

-Amylase

CNP-G

3

CALIBRATION

Calibration with a factor.

OPERATORY CONDITIONS

Introduce the following data in the computer :

LINEARITY

This method is linear up to 1000 U/L of Amylase.

TEST DEFINITION

ENTRY NAME AMI SAMPLE NORMAL 5.00

REPORT NAME AMYLASE LOW 15.00 HIGH 3.00

RATIO REF

TEST NUMBER UNITS PRIM IU/L SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG RATE KIN BLNK PRINT DIGITS 0

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 0.00000 TO 90.00000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME AMI TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG RATE KIN BLNK

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -2000.00 TO 2000.00 REF CAL FACTOR

% TOL OF CAL FACTOR 10 % TOL OF CAL 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST AMYLASE

REAGENT NAME AMI LINEARITY (C) 1.50000 TO 1200.0000

LOT ID * INITIAL Ad. 1.000

ABS LIMIT (Ad) 2.40

REAGENT VOL (uL) NORM 236 REAGENT BLANK NO

LOW 236 HIGH 336 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 60 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 180 MIX TIME (SEC) 1.00

NUMBER OF READS 3 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

404 / 6060 A 1.00 8.00 .000 .000

Bilirrubina (Total y Directa)

DMSO

CALIBRACIÓN

Utilizar un suero de calibración adecuado

CONDICIONES OPERATORIAS

Introducir en la memoria del ordenador los siguientes parámetros:

** Introducir el valor asignado al calibrador

LINEALIDAD

La técnica es lineal al menos hasta 20 mg/dL de Bilirubina.

TEST DEFINITION

ENTRY NAME BIL SAMPLE NORMAL 25.0

REPORT NAME BILIRRUBINA LOW 25.00 HIGH 10.0

RATIO REF

TEST NUMBER UNITS PRIM MG/DL SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG END PT PRINT DIGITS 1

REACTION DIRECTION UP

REAGENTS 2 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 0.00000 TO 1.00000

SERUM BLANK REAGENT BLANK

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME BIL TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG END PT

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -1000.00 TO 1000.00 REF CAL FACTOR

% TOL OF CAL FACTOR 10 % TOL OF CAL 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

SPINTROL --- 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST BILIRRUBINA

REAGENT NAME BIL LINEARITY (C) 0.00000 TO 25.00000

LOT ID * INITIAL Ad. .500

ABS LIMIT (Ad) 2.40

REAGENT VOL (uL) NORM 236

LOW 236 HIGH 236 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 120 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 120 MIX TIME (SEC) 1.00

NUMBER OF READS 1 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 180.0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

548 / 000 A 1.00 .000 .000 .000

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 2 FOR TEST BILIRRUBINA

REAGENT NAME BIL LINEARITY (C) 0.00000 TO 25.00000

LOT ID * INITIAL Ad. .500

ABS LIMIT (Ad) 2.40

REAGENT VOL (uL) NORM 5

LOW 0 HIGH 0 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 300 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 300 MIX TIME (SEC) 1.00

NUMBER OF READS 1 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0.0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

548 / 000 A 1.00 .000 .000 .000

Bilirubin (Total and Direct)

DMSO

CALIBRATION

Use a human serum calibrator

OPERATORY CONDITIONS

Introduce the following data in the computer :

** Assign the values of controls and calibrator.

LINEARITY

This method is linear up to 20 mg/dL of Bilirubin.

TEST DEFINITION

ENTRY NAME BIL SAMPLE NORMAL 25.0

REPORT NAME BILIRUBIN LOW 25.00 HIGH 10.0

RATIO REF

TEST NUMBER UNITS PRIM MG/DL SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG END PT PRINT DIGITS 1

REACTION DIRECTION UP

REAGENTS 2 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 0.00000 TO 1.00000

SERUM BLANK REAGENT BLANK

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME BIL TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG END PT

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -1000.00 TO 1000.00 REF CAL FACTOR

% TOL OF CAL FACTOR 10 % TOL OF CAL 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

SPINTROL ** 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST BILIRUBIN

REAGENT NAME BIL LINEARITY (C) 0.00000 TO 25.00000

LOT ID * INITIAL Ad. .500

ABS LIMIT (Ad) 2.40

REAGENT VOL (uL) NORM 236

LOW 236 HIGH 236 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 120 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 120 MIX TIME (SEC) 1.00

NUMBER OF READS 1 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 180.0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

548 / 000 A 1.00 .000 .000 .000

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 2 FOR TEST BILIRUBIN

REAGENT NAME BIL LINEARITY (C) 0.00000 TO 25.00000

LOT ID * INITIAL Ad. .500

ABS LIMIT (Ad) 2.40

REAGENT VOL (uL) NORM 5

LOW 0 HIGH 0 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 300 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 300 MIX TIME (SEC) 1.00

NUMBER OF READS 1 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

Calcio

o-Cresolftaleina

PREPARACIÓN Y ESTABILIDAD

Mezclar proporcionalmente 1:1 los reactivos 1 y 2. Este monoreactivo es estable 2 dias a 2-8ºC.

CALIBRACIÓN

Utilizar un suero de calibración adecuado.

CONDICIONES OPERATORIAS

Introducir en la memoria del ordenador los siguientes parámetros:

** Introducir el valor asignado al calibrador

LINEALIDAD

La técnica es lineal al menos hasta 16 mg/dL de Calcio.

TEST DEFINITION

ENTRY NAME CA SAMPLE NORMAL 2.50

REPORT NAME CALCIO LOW 5.00 HIGH 1.25

RATIO REF

TEST NUMBER UNITS PRIM MG/DL SEC MMOL/L

TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.2500

MATH LIN REG END PT PRINT DIGITS 1

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 9.00000 TO 11.00000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME CA TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG END PT

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -2000.00 TO 2000.00 REF CAL FACTOR

% TOL OF CAL FACTOR 10 % TOL OF CAL 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

SPINTROL ** 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST CALCIO

REAGENT NAME CA LINEARITY (C) 2.00000 TO 16.0000

LOT ID * INITIAL Ad. .500

ABS LIMIT (Ad) 2.40

REAGENT VOL (uL) NORM 236 REAGENT BLANK YES

LOW 236 HIGH 286 BEFORE WASH CYCLES 2

FIRST READ TIME (SEC) 120 AFTER WASH CYCLES 3

LAST READ TIME (SEC) 180 MIX TIME (SEC) 1.00

NUMBER OF READS 1 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

572 / 660 A 1.00 .000 .000

Calcium

o-Cresolphtalein

PREPARATION AND STABILITY

Mix proportionally 1:1 both reagents.

The working reagent is stable 2 days at 2-8ºC.

CALIBRATION

Use a human serum calibrator

OPERATORY CONDITIONS

Introduce the following data in the computer :

** Assign the values of controls and calibrator.

LINEARITY

This method is linear up to 16 mg/dL of Calcium.

TEST DEFINITION

ENTRY NAME CA SAMPLE NORMAL 2.50

REPORT NAME CALCIUM LOW 5.00 HIGH 1.25

RATIO REF

TEST NUMBER UNITS PRIM MG/DL SEC MMOL/L

TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.2500

MATH LIN REG END PT PRINT DIGITS 1

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 9.00000 TO 11.00000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME CA TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG END PT

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -2000.00 TO 2000.00 REF CAL FACTOR

% TOL OF CAL FACTOR 10 % TOL OF CAL 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

SPINTROL ** 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST CALCIUM

REAGENT NAME CA LINEARITY (C) 2.00000 TO 16.0000

LOT ID * INITIAL Ad. .500

ABS LIMIT (Ad) 2.40

REAGENT VOL (uL) NORM 236 REAGENT BLANK YES

LOW 236 HIGH 286 BEFORE WASH CYCLES 2

FIRST READ TIME (SEC) 120 AFTER WASH CYCLES 3

LAST READ TIME (SEC) 180 MIX TIME (SEC) 1.00

NUMBER OF READS 1 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

572 / 660 A 1.00 .000

Cloruros

Tiocianato

CALIBRACIÓN

Utilizar un suero de calibración adecuado

CONDICIONES OPERATORIAS

Introducir en la memoria del ordenador los siguientes parámetros:

** Introducir el valor asignado al calibrador

LINEALIDAD

La técnica es lineal al menos hasta 130 mEq/L de Cloruros.

TEST DEFINITION

ENTRY NAME CL SAMPLE NORMAL 2.50

REPORT NAME CLORUROS LOW 3.25 HIGH 2.25

RATIO REF

TEST NUMBER UNITS PRIM mEQ/L SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG END PT PRINT DIGITS 0

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 98 TO 107

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME CL TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG END PT

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -2000.00 TO 2000.00 REF CAL FACTOR

% TOL OF CAL FACTOR 10 % TOL OF CAL 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

SPINTROL ** 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST CLORUROS

REAGENT NAME CL LINEARITY (C) 0 TO 130

LOT ID * INITIAL Ad. 0.400

ABS LIMIT (Ad) 2.400

REAGENT VOL (uL) NORM 236 REAGENT BLANK NO

LOW 236 HIGH 286 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 120 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 180 MIX TIME (SEC) 1.00

NUMBER OF READS 1 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

484 / 604 A 1.00 .000 .000

Chloride

Thiocyanate

CALIBRATION

Use a human serum calibrator

OPERATORY CONDITIONS

Introduce the following data in the computer :

** Assign the values of controls and calibrator.

LINEARITY

This method is linear up to 130 mEq/L of Chloride.

TEST DEFINITION

ENTRY NAME CL SAMPLE NORMAL 2.50

REPORT NAME CHLORIDE LOW 3.25 HIGH 2.25

RATIO REF

TEST NUMBER UNITS PRIM mEQ/L SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG END PT PRINT DIGITS 0

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 98 TO 107

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME CL TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG END PT

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -2000.00 TO 2000.00 REF CAL FACTOR

% TOL OF CAL FACTOR 10 % TOL OF CAL 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

SPINTROL ** 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST CHLORIDE

REAGENT NAME CL LINEARITY (C) 0 TO 130

LOT ID * INITIAL Ad. 0.400

ABS LIMIT (Ad) 2.400

REAGENT VOL (uL) NORM 236 REAGENT BLANK NO

LOW 236 HIGH 286 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 120 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 180 MIX TIME (SEC) 1.00

NUMBER OF READS 1 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

484 / 604 A 1.00 .000 .000

Colesterol

CE-CO-POD

CALIBRACIÓN

Utilizar un suero de calibración adecuado

CONDICIONES OPERATORIAS

Introducir en la memoria del ordenador los siguientes parámetros:

** Introducir el valor asignado al calibrador

LINEALIDAD

La técnica es lineal al menos hasta 800 mg/dL de Colesterol.

TEST DEFINITION

ENTRY NAME COL SAMPLE NORMAL 2.50

REPORT NAME COLESTEROL LOW 5.00 HIGH 1.25

RATIO REF

TEST NUMBER UNITS PRIM MG/DL SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG END PT PRINT DIGITS 0

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 120.00000 TO 250.0000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME COL TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG END PT

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -2000.00 TO 2000.00 REF CAL FACTOR

% TOL OF CAL FACTOR 10 % TOL OF CAL 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

CAL-HDL ** 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST COLESTEROL

REAGENT NAME COL LINEARITY (C) 0.00000 TO 800.0000

LOT ID * INITIAL Ad. .200

ABS LIMIT (Ad) 2.20

REAGENT VOL (uL) NORM 236 REAGENT BLANK YES

LOW 236 HIGH 286 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 240 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 300 MIX TIME (SEC) 1.00

NUMBER OF READS 1 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

516 / 600 A 1.00 .000 .000

Cholesterol

CE-CO-POD

CALIBRATION

Use a human serum calibrator

OPERATORY CONDITIONS

Introduce the following data in the computer :

* Assign the values of controls and calibrator.

LINEARITY

This method is linear up to 800 mg/dL of Cholesterol.

TEST DEFINITION

ENTRY NAME CHOL SAMPLE NORMAL 2.50

REPORT NAME CHOLESTEROL LOW 5.00 HIGH 1.25

RATIO REF

TEST NUMBER UNITS PRIM MG/DL SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG END PT PRINT DIGITS 0

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 120.00000 TO 250.0000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME CHOL TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG END PT

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -2000.00 TO 2000.00 REF CAL FACTOR

% TOL OF CAL FACTOR 10 % TOL OF CAL 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

CAL-HDL ** 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST CHOLESTEROL

REAGENT NAME CHOL LINEARITY (C) 0.00000 TO 800.0000

LOT ID * INITIAL Ad. .200

ABS LIMIT (Ad) 2.20

REAGENT VOL (uL) NORM 236 REAGENT BLANK YES

LOW 236 HIGH 286 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 240 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 300 MIX TIME (SEC) 1.00

NUMBER OF READS 1 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

516 / 600 A 1.00 .000 .000

CK-MB

Inmunoinhibición

CALIBRACIÓN

Calibración frente a factor.

CONDICIONES OPERATORIAS

Introducir en la memoria del ordenador los siguientes parámetros:

** Introducir el valor asignado al calibrador

TEST DEFINITION

ENTRY NAME CK SAMPLE NORMAL 5.00

REPORT NAME CK-MB LOW 15.00 HIGH 3.00

RATIO REF

TEST NUMBER UNITS PRIM IU/L SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG RATE KIN BLNK PRINT DIGITS 0

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 24.00000 TO 195.00000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME CK TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG RATE KIN BLNK

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -1000.00 TO 1000.00

% TOL OF CAL FACTOR 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST CK-MBC

REAGENT NAME CK LINEARITY (C) 5.0000 TO 1000.0000

LOT ID * INITIAL Ad. 0.500

ABS LIMIT (Ad) 2.40

REAGENT VOL (uL) NORM 236 REAGENT BLANK NO

LOW 236 HIGH 336 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 180 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 300 MIX TIME (SEC) 1.00

NUMBER OF READS 3 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

340 / 380 A 1.00 5.47 .000 .000

CK-MB

Immunoinhibition

CALIBRATION

Calibration in front of a factor.

OPERATORY CONDITIONS

Introduce the following data in the computer :

* Assign the values of controls and calibrator.

TEST DEFINITION

ENTRY NAME CK SAMPLE NORMAL 5.00

REPORT NAME CK-MB LOW 15.00 HIGH 3.00

RATIO REF

TEST NUMBER UNITS PRIM IU/L SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG RATE KIN BLNK PRINT DIGITS 0

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 24.00000 TO 195.00000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME CK TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG RATE KIN BLNK

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -1000.00 TO 1000.00

% TOL OF CAL FACTOR 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST CK-MB

REAGENT NAME CK LINEARITY (C) 5.0000 TO 1000.0000

LOT ID * INITIAL Ad. 0.500

ABS LIMIT (Ad) 2.40

REAGENT VOL (uL) NORM 236 REAGENT BLANK NO

LOW 236 HIGH 336 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 180 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 300 MIX TIME (SEC) 1.00

NUMBER OF READS 3 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

340 / 380 A 1.00 5.47 .000 .000

CK-NAC

IFCC

CALIBRACIÓN

Calibración frente a factor.

CONDICIONES OPERATORIAS

Introducir en la memoria del ordenador los siguientes parámetros:

LINEALIDAD

La técnica es lineal al menos hasta 1000 U/L de CK.

TEST DEFINITION

ENTRY NAME CK SAMPLE NORMAL 5.00

REPORT NAME CK-NAC LOW 15.00 HIGH 3.00

RATIO REF

TEST NUMBER UNITS PRIM IU/L SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG RATE KIN BLNK PRINT DIGITS 0

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 24.00000 TO 195.00000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME CK TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG RATE KIN BLNK

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -1000.00 TO 1000.00

% TOL OF CAL FACTOR 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST CK-NAC

REAGENT NAME CK LINEARITY (C) 5.0000 TO 1000.0000

LOT ID * INITIAL Ad. 0.500

ABS LIMIT (Ad) 2.40

REAGENT VOL (uL) NORM 236 REAGENT BLANK NO

LOW 236 HIGH 336 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 180 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 300 MIX TIME (SEC) 1.00

NUMBER OF READS 3 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

340 / 380 A 1.00 5.47 .000 .000

CK-NAC

IFCC

CALIBRATION

Calibration with a factor.

OPERATORY CONDITIONS

Introduce the following data in the computer :

* Assign the values of controls and calibrator.

LINEARITY

This method is linear up to 1000 U/L of CK.

TEST DEFINITION

ENTRY NAME CK SAMPLE NORMAL 5.00

REPORT NAME CK-NAC LOW 15.00 HIGH 3.00

RATIO REF

TEST NUMBER UNITS PRIM IU/L SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG RATE KIN BLNK PRINT DIGITS 0

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 24.00000 TO 195.00000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME CK TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG RATE KIN BLNK

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -1000.00 TO 1000.00

% TOL OF CAL FACTOR 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST CK-NAC

REAGENT NAME CK LINEARITY (C) 5.0000 TO 1000.0000

LOT ID * INITIAL Ad. 0.500

ABS LIMIT (Ad) 2.40

REAGENT VOL (uL) NORM 236 REAGENT BLANK NO

LOW 236 HIGH 336 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 180 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 300 MIX TIME (SEC) 1.00

NUMBER OF READS 3 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

340 / 380 A 1.00 5.47 .000 .000

Creatinina

Jaffé

PREPARACIÓN Y ESTABILIDAD

Mezclar ambos reactivos a partes iguales según necesidades.

Esta mezcla es estable 10 días a temperatura ambiente.

CALIBRACIÓN

Utilizar un suero de calibración adecuado

CONDICIONES OPERATORIAS

Introducir en la memoria del ordenador los siguientes parámetros:

** Introducir el valor asignado al calibrador

LINEALIDAD

La técnica es lineal al menos hasta 15 mg/dL de Creatinina.

TEST DEFINITION

ENTRY NAME CRE SAMPLE NORMAL 20.00

REPORT NAME CREATININA LOW 10.00 HIGH 15.00

RATIO REF

TEST NUMBER UNITS PRIM MG/DL SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG END PT PRINT DIGITS 0

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 0.70000 TO 1.40000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME CRE TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG END PT

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -2000.00 TO 2000.00 REF CAL FACTOR

% TOL OF CAL FACTOR 10 % TOL OF CAL 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

SPINTROL ** 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST CREATININA

REAGENT NAME CRE LINEARITY (C) 0.20000 TO 15.0000

LOT ID * INITIAL Ad. 0.600

ABS LIMIT (Ad) 2.400

REAGENT VOL (uL) NORM 236 REAGENT BLANK YES

LOW 236 HIGH 286 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 30 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 120 MIX TIME (SEC) 1.00

NUMBER OF READS 2 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

484 / 604 A 1.00 .000 .000

Creatinine

Jaffé

PREPARATION AND STABILITY

Mix proportionally 1:1 both reagents.

This working reagent is stable 10 days at room temperature.

CALIBRATION

Use a human serum calibrator

OPERATORY CONDITIONS

Introduce the following data in the computer :

* Assign the values of controls and calibrator.

LINEARITY

This method is linear up to 15 mg/dL of Creatinine.

TEST DEFINITION

ENTRY NAME CRE SAMPLE NORMAL 20.00

REPORT NAME CREATININE LOW 10.00 HIGH 15.00

RATIO REF

TEST NUMBER UNITS PRIM MG/DL SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG END PT PRINT DIGITS 0

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 0.70000 TO 1.40000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME CRE TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG END PT

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -2000.00 TO 2000.00 REF CAL FACTOR

% TOL OF CAL FACTOR 10 % TOL OF CAL 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

SPINTROL ** 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST CREATININE

REAGENT NAME CRE LINEARITY (C) 0.20000 TO 15.0000

LOT ID * INITIAL Ad. 0.600

ABS LIMIT (Ad) 2.400

REAGENT VOL (uL) NORM 236 REAGENT BLANK YES

LOW 236 HIGH 286 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 30 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 120 MIX TIME (SEC) 1.00

NUMBER OF READS 2 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

484 / 604 A 1.00 .000 .000

Hierro

Ferrozine

CALIBRACIÓN

Utilizar un suero de calibración adecuado

CONDICIONES OPERATORIAS

Introducir en la memoria del ordenador los siguientes parámetros:

TEST DEFINITION

ENTRY NAME FE SAMPLE NORMAL 25.0

REPORT NAME HIERRO LOW 25.0 HIGH 10.0

RATIO REF

TEST NUMBER UNITS PRIM uG/DL SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG END PT. PRINT DIGITS 0

REACTION DIRECTION UP

REAGENTS 2 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 37.0000 TO 156.000

SERUM BLANK REAGENT 1 BLANK

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME FE TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG END PT.

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -2000.00 TO 2000.00 REF CAL FACTOR

% TOL OF CAL FACTOR 10 % TOL OF CAL 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

SPINTROL ** 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST HIERRO

REAGENT NAME FE LINEARITY (C) 10.000 TO 1000.000

LOT ID * INITIAL Ad. 0.500

ABS LIMIT (Ad) 2.40

REAGENT VOL (uL) NORM 236 REAGENT BLANK NO

LOW 236 HIGH 236 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 120 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 120 MIX TIME (SEC) 1.00

NUMBER OF READS 1 COOLING YES

READ INTERVAL (SEC) 60 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 180.0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

564 / 660 A 1.00 .000 .000 .000

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 2 FOR TEST HIERRO

REAGENT NAME FE LINEARITY (C) 10.000 TO 1000.000

LOT ID * INITIAL Ad. 0.500

ABS LIMIT (Ad) 2.20

REAGENT VOL (uL) NORM 5 REAGENT BLANK NO

LOW 5 HIGH 0 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 300 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 300 MIX TIME (SEC) 1.00

NUMBER OF READS 1 COOLING YES

READ INTERVAL (SEC) 60 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0.0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

564 / 660 A 1.00 .000 .000

Iron

Ferrozine

CALIBRATION

Use a human serum calibrator

OPERATORY CONDITIONS

Introduce the following data in the computer :

** Assign the values of controls and calibrator.

LINEARITY

This method is linear up to 700

µ

g/dL of Iron.

TEST DEFINITION

ENTRY NAME FE SAMPLE NORMAL 25.0

REPORT NAME IRON LOW 25.0 HIGH 10.0

RATIO REF

TEST NUMBER UNITS PRIM uG/DL SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG END PT. PRINT DIGITS 0

REACTION DIRECTION UP

REAGENTS 2 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 37.0000 TO 156.000

SERUM BLANK REAGENT 1 BLANK

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME FE TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG END PT.

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -2000.00 TO 2000.00 REF CAL FACTOR

% TOL OF CAL FACTOR 10 % TOL OF CAL 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

SPINTROL ** 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST IRON

REAGENT NAME FE LINEARITY (C) 10.000 TO 1000.000

LOT ID * INITIAL Ad. 0.500

ABS LIMIT (Ad) 2.40

REAGENT VOL (uL) NORM 236 REAGENT BLANK NO

LOW 236 HIGH 236 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 120 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 120 MIX TIME (SEC) 1.00

NUMBER OF READS 1 COOLING YES

READ INTERVAL (SEC) 60 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 180.0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

564 / 660 A 1.00 .000 .000 .000

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 2 FOR TEST IRON

REAGENT NAME FE LINEARITY (C) 10.000 TO 1000.000

LOT ID * INITIAL Ad. 0.500

ABS LIMIT (Ad) 2.20

REAGENT VOL (uL) NORM 5 REAGENT BLANK NO

LOW 5 HIGH 0 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 300 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 300 MIX TIME (SEC) 1.00

NUMBER OF READS 1 COOLING YES

READ INTERVAL (SEC) 60 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0.0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

564 / 660 A 1.00 .000 .000

γ

γ

γ

γ

-GT

IFCC

CALIBRACIÓN

Calibración frente a un Factor.

CONDICIONES OPERATORIAS

Introducir en la memoria del ordenador los siguientes parámetros:

LINEALIDAD

La técnica es lineal al menos hasta 400 U/L de

γ

-GT.

TEST DEFINITION

ENTRY NAME GGT SAMPLE NORMAL 20.00

REPORT NAME GAMMA-GT LOW 25.00 HIGH 5.00

RATIO REF

TEST NUMBER UNITS PRIM IU/L SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG RATE KIN BLNK PRINT DIGITS 0

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 5.00000 TO 50.0000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME GGT TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG RATE KIN BLNK

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -1000.00 TO 1000.00

% TOL OF CAL FACTOR 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST GAMMA-GT

REAGENT NAME GGT LINEARITY (C) 5.0000 TO 300.0000

LOT ID * INITIAL Ad. 0.600

ABS LIMIT (Ad) 2.200

REAGENT VOL (uL) NORM 236 REAGENT BLANK NO

LOW 236 HIGH 236 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 60 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 180 MIX TIME (SEC) 1.00

NUMBER OF READS 3 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

404 / 660 A 1.00 9.2 .000 .000

γ

γ

γ

γ

-GT

IFCC

CALIBRATION

Calibration in front of a Factor.

OPERATORY CONDITIONS

Introduce the following data in the computer :

* Assign the values of controls and calibrator.

LINEARITY

This method is linear up to 400 U/L of

γ

-GT.

TEST DEFINITION

ENTRY NAME GGT SAMPLE NORMAL 20.00

REPORT NAME GAMMA-GT LOW 25.00 HIGH 5.00

RATIO REF

TEST NUMBER UNITS PRIM IU/L SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG RATE KIN BLNK PRINT DIGITS 0

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 5.00000 TO 50.0000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME GGT TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG RATE KIN BLNK

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -1000.00 TO 1000.00

% TOL OF CAL FACTOR 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST GAMMA-GT

REAGENT NAME GGT LINEARITY (C) 5.0000 TO 300.0000

LOT ID * INITIAL Ad. 0.600

ABS LIMIT (Ad) 2.200

REAGENT VOL (uL) NORM 236 REAGENT BLANK NO

LOW 236 HIGH 236 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 60 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 180 MIX TIME (SEC) 1.00

NUMBER OF READS 3 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

404 / 660 A 1.00 9.2 .000 .000

Glucosa

GOD-POD

CALIBRACIÓN

Utilizar un suero de calibración adecuado.

CONDICIONES OPERATORIAS

Introducir en la memoria del ordenador los siguientes parámetros:

** Introducir el valor asignado al calibrador

LINEALIDAD

La técnica es lineal al menos hasta 500 mg/dL de Glucosa.

TEST DEFINITION

ENTRY NAME GLU SAMPLE NORMAL 2.50

REPORT NAME GLUCOSA LOW 5.00 HIGH 1.25

RATIO REF

TEST NUMBER UNITS PRIM MG/DL SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG END PT. PRINT DIGITS 0

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 60.0000 TO 110.000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME GLU TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG END PT.

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -1000.00 TO 1000.00 REF CAL FACTOR

% TOL OF CAL FACTOR 10 % TOL OF CAL 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

SPINTROL ** 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST GLUCOSA

REAGENT NAME GLU LINEARITY (C) 50.000 TO 500.000

LOT ID * INITIAL Ad. 0.500

ABS LIMIT (Ad) 2.20

REAGENT VOL (uL) NORM 236 REAGENT BLANK YES

LOW 236 HIGH 286 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 420 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 420 MIX TIME (SEC) 1.00

NUMBER OF READS 1 COOLING YES

READ INTERVAL (SEC) 60 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

500 / 660 A 1.00 .000 .000

Glucose

GOD-POD

CALIBRATION

Use a human serum calibrator

OPERATORY CONDITIONS

Introduce the following data in the computer :

** Assign the values of controls and calibrator.

LINEARITY

This method is linear up to 500 mg/dL of Glucose.

TEST DEFINITION

ENTRY NAME GLU SAMPLE NORMAL 2.50

REPORT NAME GLUCOSE LOW 5.00 HIGH 1.25

RATIO REF

TEST NUMBER UNITS PRIM MG/DL SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG END PT. PRINT DIGITS 0

REACTION DIRECTION UP

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 60.0000 TO 110.000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME GLU TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG END PT.

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -1000.00 TO 1000.00 REF CAL FACTOR

% TOL OF CAL FACTOR 10 % TOL OF CAL 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

SPINTROL ** 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST GLUCOSE

REAGENT NAME GLU LINEARITY (C) 50.000 TO 500.000

LOT ID * INITIAL Ad. 0.500

ABS LIMIT (Ad) 2.20

REAGENT VOL (uL) NORM 236 REAGENT BLANK YES

LOW 236 HIGH 286 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 420 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 420 MIX TIME (SEC) 1.00

NUMBER OF READS 1 COOLING YES

READ INTERVAL (SEC) 60 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

500 / 660 A 1.00 .000 .000

GOT / AST

IFCC

CALIBRACIÓN

Utilizar un suero de calibración adecuado

CONDICIONES OPERATORIAS

Introducir en la memoria del ordenador los siguientes parámetros:

** Introducir el valor asignado al calibrador

LINEALIDAD

La técnica es lineal al menos hasta 450 U/L de GOT.

TEST DEFINITION

ENTRY NAME GOT SAMPLE NORMAL 20.00

REPORT NAME GOT LOW 25.00 HIGH 5.00

RATIO REF

TEST NUMBER UNITS PRIM IU/L SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG RATE KIN BLNK PRINT DIGITS 0

REACTION DIRECTION DOWN

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 0.00000 TO 40.0000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME GOT TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG RATE KIN BLNK

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -2000.00 TO 2000.00

% TOL OF CAL FACTOR 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST GOT

REAGENT NAME GOT LINEARITY (C) 5.0000 TO 500.0000

LOT ID * INITIAL Ad. 0.800

ABS LIMIT (Ad) 0.360

REAGENT VOL (uL) NORM 236 REAGENT BLANK NO

LOW 236 HIGH 336 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 120 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 240 MIX TIME (SEC) 1.00

NUMBER OF READS 3 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

340 / 380 A 1.00 5.47 .000 .000

GOT / AST

IFCC

CALIBRATION

Use a human serum calibrator

OPERATORY CONDITIONS

Introduce the following data in the computer :

* Assign the values of controls and calibrator.

LINEARITY

This method is linear up to 450 U/L of GOT.

TEST DEFINITION

ENTRY NAME GOT SAMPLE NORMAL 20.00

REPORT NAME GOT LOW 25.00 HIGH 5.00

RATIO REF

TEST NUMBER UNITS PRIM IU/L SEC

-TEST TYPE CALIBRATED SEC. UNITS FACTOR 0.00000

MATH LIN REG RATE KIN BLNK PRINT DIGITS 0

REACTION DIRECTION DOWN

REAGENTS 1 INST MUL 1.00000 INT 0.00000

TEMPERATURE (C) 37 NORMAL (C) 0.00000 TO 40.0000

SERUM BLANK NO

COMB TEST NAME SAMPLE DISP DELAY (SEC) 0

LINEAR MODEL CALIBRATION DEFINITION

TEST NAME GOT TEST TYPE CALIBRATED

COMB TEST MATH MODEL LIN REG RATE KIN BLNK

CAL MODE CAL ON CMD CAL INTERVAL (HR) 255

INTCPT TOL (C) -2000.00 TO 2000.00

% TOL OF CAL FACTOR 10

CAL LEVEL 0

CALIBRATOR LEVEL REPLICATES

WATER 0.00000 1

TEST PARAMETER FILE : REAGENT DEFINITION REAGENT NUMBER 1 FOR TEST GOT

REAGENT NAME GOT LINEARITY (C) 5.0000 TO 500.0000

LOT ID * INITIAL Ad. 0.800

ABS LIMIT (Ad) 0.360

REAGENT VOL (uL) NORM 236 REAGENT BLANK NO

LOW 236 HIGH 336 BEFORE WASH CYCLES 5

FIRST READ TIME (SEC) 120 AFTER WASH CYCLES 5

LAST READ TIME (SEC) 240 MIX TIME (SEC) 1.00

NUMBER OF READS 3 COOLING YES

READ INTERVAL (SEC) 60.0 CONSTANT INTERCEPT 0.00000

AUX REAG DISP (SEC) 0 CORRECTION LIMIT 0.00000

RSM 0 SPECTRAL CORRECTION

PRIM / SEC USE IN CONST E.F. LOW HIGH

340 / 380 A 1.00 5.47 .000 .000