Europass Curriculum Vitae

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Europass

Curriculum Vitae

Personal information

First name(s) / Surname(s)

Maria Manuel Sampaio Cristóvão

Address Rua da Bélgica, n.25 4ºDto, 2775-404 Carcavelos (Portugal)

Telephone(s) +351 214582765 Mobile +351 933265495

E-mail(s) [email protected] Nationality Portuguese

Date of birth 20 September 1976 Gender Female

Desired employment /

Occupational field

Scientific Researcher

Work experience

Dates 15/09/2005 → Occupation or position held

_Professor

Main activities and responsibilities Teaching several subjects to degree students, such as: Molecular Biology, Microbiology, Introduction to Biotechnology, Genetic Engineering, Bioinformatic, Biochemistry, Project in Biotechnology, Cellular Biology, Methods in Analytic Chemistry

Teaching in Master Programs: MEIO Master (Integrated Studies of the Oceans), Master in Marine Resources, Master in Aquaculture

Course Coordinator - Marine Biology and Biotechnology (2008-2010) Course Coordinator - Tourism (2006-2008)

Member of the Scientific-Pedagogical Commission from the Marine Biology and Biotechnology Course Member of the Scientific Commission from the School of Tourism and Maritime Technology, ESTM – IPL

Member of the Marine Resources Research Group Name and address of employer Polytechnic Institute of Leiria - ESTM

Campus 4 Santuário de Nossa Senhora dos Remédios, 2520–641 Peniche (Portugal) Type of business or sector Higher Education

Dates 2000 - 2005 Occupation or position held

_{PhD student}

Main activities and responsibilities PhD in Biochemical Engineering and Biotechnology, area of Biochemistry, done under the supervision of Professor Maria Helena Dias dos Santos and Professor Winfried Boos. The work was developed in Instituto de Tecnologia Química e Biológica – UNL, together with the Faculty of Biology – University of Konstanz, Germany;

• Work plan integrated in the European Project “Exploiting New Solutes from

Hyperthermophiles for the Preservation of Biomaterials: Cell Factories for the Production of Hypersolutes”;

•

Techniques such as nucleic acid purification (DNA and RNA), protein and DNA electrophoreses, cloning and construction of plasmid vectors, construction of metabolic altered strains, protein purification, chromatography, identification and quantification of compounds by Nuclear Magnetic Resonance (NMR), synthesis of radioactive (14_C)

compounds, culture of mesophilic, thermophilic and hyperthermophilic organisms, were developed under the PhD work plan.

Name and address of employer Universidade Nova de Lisboa (University of Lisbon) - ITQB

Av. da República, Estação Agronómica Nacional, 2780-157 Oeiras (Portugal) Type of business or sector Research Center

Dates 1998 - 2000 Occupation or position held

_{Research Student}

Main activities and responsibilities Working in a research group, in order to obtain scientific training in research projects and in science and technology institutions.

Projects:

"Strategies for Survival of Strictly Anaerobic Bacteria in environments containing oxygen"

• This work involved the development of growth techniques for sulphate reducing

bacteria, purification and characterization of reserve polymers, metabolism and enzyme characterization.

“Towards the Utilization of Cell-Reactors for the Production of Mannosylglycerate, an Enzyme Stabilizer from Hyperthermophiles”

• The final objective was the identification and utilization of compatible solutes as

stabilizers of biomaterials, namely enzymes, proteins, vaccines, cells and animal tissues.

“New compatible solutes from thermophiles and hyperthermophiles: screening, identification and physiological role”

Name and address of employer UNL - ITQB

Av. da República, Estação Agronómica Nacional, 2780-157 Oeiras (Portugal) Type of business or sector Research Center

Education and training

Dates 2000 - 06/2005 Title of qualification awarded

_PhD

Principal subjects / occupational skills covered

Thesis Title: “Engineering Escherichia coli for the Synthesis of Mannosylglycerate, a solute widely distributed in (hyper)thermophiles. Transport, Metabolism and Physiological Role”

PhD in Biochemical Engineering and Biotechnology, area of Biochemistry, done under the supervision of Professor Maria Helena Dias dos Santos and Professor Winfried Boos. The work was developed in Instituto de Tecnologia Química e Biológica – UNL, together with the Faculty of Biology – University of Konstanz, Germany

Biochemistry, Microbiology, Biotechnology, Genetic Engineering, Molecular Biology Name and type of organisation

providing education and training

UNL - ITQB (Institute of Lisbon University)

Av. da República, Estação Agronómica Nacional, 2780-157 Oeiras (Portugal) Level in national or international

classification

ISCED 8

Dates 09/1993 - 06/1998 Title of qualification awarded

Degree in Microbiology

Principal subjects / occupational skills covered

Biology, Chemistry, Biotechnology, Genetics, Analytical Methods, Molecular Biology, Thermodinamics,

Page 3 / 5 - Curriculum vitae of For more information on Europass go to http://europass.cedefop.europa.eu Name and type of organisation

providing education and training

Escola Superior de Biotecnologia - UCP (University)

Rua Dr. António Bernardino de Almeida, 4200-072 Porto (Portugal) Level in national or international

classification

ISCED 5

Dates 2006 - 2007

Title of qualification awarded Superior Technical Health, Safety and Health at Work” Principal subjects / occupational skills

covered

Design and management training; legislation, regulations and standards; organizational management; social psychology at work; techniques of information, communication and negotiation; prevention management; occupational hygiene; ergonomics; safety; risk assessment; risk control; organization of emergency; audits.

Name and type of organisation providing education and training

TÜV Akademie Rheinland (Certification Center)

Arquiparque, Edifício Zenith. Rua Dr. António Loureiro Borges, 9, 3º, 1495-131 Miraflores (Portugal) Level in national or international

classification

544 h course

Personal skills and

competences

Other language(s)

Self-assessment

Understanding

Speaking

W r i t i n g

European level (*) Listening Reading Spoken interaction Spoken production

English

C2 Proficient user C2 Proficient user C2 Proficient user C2 Proficient user C1 Proficient user

French

B1 Independent user B1 Independent user A2 Basic User A1 Basic User A1 Basic User

Spanish / Castilian

B1 Independent user B1 Independent user A1 Basic User A1 Basic User A1 Basic User

(*) Common European Framework of Reference (CEF) level

Social skills and competences Team spirit.

Ability to work in various types of team projects. Good ability to adapt to multicultural environments. Good communication skills.

Organisational skills and competences Sense of organisation and plan elaboration.

Good knowledge of higher education burocratic procedures. Large experience in evaluating curricular plans from several degrees. Organisation of seminars.

Team organisation.

Technical skills and competences Good knowledge of microbiological, biotechnological and molecular biology technics. Computer skills and competences Competent with most Microsoft Office programmes.

Familiar with Bioinformatic tools. Artistic skills and competences Photo, crafts.

Driving licence(s) B

Additional information

PUBLICATIONS

Noronha M, Gerbelová H, Faria TQ, Sampaio MM, Rudolph R, Maçanita AL, Santos H. Enhancing the fluorescence of tyr-59 in ubiquitin by blocking proton transfer. (2009) Phys Chem Chem Phys. 14;11(18):3580-3.

Jorge D. C, Sampaio M-M., Hreggvidsson G. Ó., Kristjánson J. K. & Santos H. A highly thermostable trehalase from the thermophilic bacterium Rhodothermus marinus. (2007) Extremophiles, 11(1): 115-22.

Silva Z, Sampaio MM, Henne A, Böhm A, Gutzat R, Boos W, da Costa MS, Santos H. The high-affinity maltose/trehalose ABC transporter in the extremely thermophilic bacterium Thermus thermophilus HB27 also recognizes sucrose and palatinose. (2005) J Bacteriol. 187(4):1210-8. Sampaio MM, Chevance F, Dippel R, Eppler T, Schlegel A, Boos W, Lu YJ, Rock CO. Phosphotransferase-mediated transport of the osmolyte 2-O-alpha-mannosyl-D-glycerate in Escherichia coli occurs by the product of the mngA (hrsA) gene and is regulated by the mngR (farR) gene product acting as repressor. (2004) J Biol Chem. 13;279(7):5537-48.

Sampaio MM, Santos H, Boos W. Synthesis of GDP-mannose and mannosylglycerate from labeled mannose by genetically engineered Escherichia coli without loss of specific isotopic enrichment. (2003) Appl Environ Microbiol. 69(1):233-40.

PROCEEDINGS OF INTERNATIONAL MEETINGS

Mendonça E, Cruz A, Neves-Afonso C, Sampaio M (2008) Genetic characterization through RAPD of Armeria spp. from Berlengas Natural Park and Peniche coast. ESBES 7 - 7th European Symposium on Biochemical Engineering Science, Universidade do Algarve, Faro, 7-10 September

Mendes MJ, Lopes N, Capitão A, Gomes D, Cruz A, Mouga TM, Neves C, Sampaio M-M (2007) Germoplasm bank construction and genetic characterization of the endemic flora from Berlengas Natural Park through RAPD. Journal of Biotechnology, 131: S32

Pires V, Matos I, Cruz A, Mouga TM, Sampaio M-M, Leandro S, Neves C (2007) Micropropagation of selected native plants from Peniche area. Journal of Biotechnology, 131: S33

POSTERS

Barros I, Duarte MM, Elias L, Cruz A, Afonso C, Pedrosa R, Sampaio MM (2009). Evaluating Bioactive Compounds extracted from Anemones living on the Peniche Coast and on Berlengas Natural Reserve. International Meeting on Marine Resources, Peniche, 16-18 November.

Pinteus S, Alves C, Rodrigues AI, Azevedo S, Sampaio MM, Afonso C, Cruz A, Mouga T, Pedrosa R (2009). High antioxidant potential of Fucus spiralis and Enteromorpha compressa extracts collected from Peniche Coast (Portugal). International Meeting on Marine Resources, Peniche, 16-18 November.

Pinteus S, Azevedo S, Alves C, Rodrigues AI, Mouga T, Cruz A, Afonso C, Sampaio MM, Pedrosa R (2009). High antioxidant potential of Fucus spiralis extracts collected from Peniche coast (Portugal). 14th European Congress on Biotechnology, Barcelona, Spain 13–16 September.

Silva KJ, Santos L, Mendonca E, Cruz A, Sampaio MM, Mouga T, Afonso C (2009). In-vitro micropropagation of Pulicaria microcephala, an endemic species from Berlengas Natural Park. International Meeting on Marine Resources, Peniche, 16-18 November.

Mendonça E, Cruz A, Neves-Afonso C, Sampaio M (2008) Genetic characterization through RAPD of Armeria spp. from Berlengas Natural Park and Peniche coast. ESBES 7 - 7th European Symposium on Biochemical Engineering Science, Universidade do Algarve, Faro, 7-10 September

Mendes MJ, Lopes N, Capitão A, Gomes D, Cruz A, Mouga T, Neves C, Sampaio M-M (2007) Germoplasm bank construction and genetic characterization of the endemic flora from Berlengas Natural Park through RAPD. 13th European Congress on Biotechnology, Barcelona, Spain. 16-19

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Pires V, Matos I, Cruz A, Mouga T, Sampaio M-M, Leandro S, Neves C (2007) Micropropagation of selected native plants from Peniche area. 13th European Congress on Biotechnology, Barcelona, Spain, 16-19 September.

Jorge C, Sampaio M-M, Hreggvidsson G, Kristjánson J, Santos H (2006) A highly thermostable trehalase from the thermophilic bacterium Rhodothermus marinus. Extremophiles 2006 International Conference, Brest, France, September.

Sampaio M-M., Silva Z., da Costa M.S., Boos W. & Santos H. Transport of Mannosylglycerate and Trehalose in the Thermophilic Bacterium Thermus thermophilus. 4th International Congress on Termophiles, Exeter, UK, 2003.

Sampaio M-M., Santos H. & Boos W. Synthesis of GDP-Mannose and Mannosylglycerate by Engineered Escherichia coli without Loss of Specific Activity. 4th International Congress on Extremophiles, Naples, Italy, 2002.

Jorge C., Sampaio M-M., Mingote A., Boos W. & Santos H. Trehalose Metabolism in Rhodothermus marinus: chraterization of a very active periplasmic trehalase. 4th International Congress on Extremophiles, Naples, Italy, 2002.

Jorge C., Sampaio M-M., Boos W. & Santos H. Trehalose and Glucose Metabolism in the Thermophilic Bacterium Rhodothermus marinus. Micro’2001, Congresso Nacional de Microbiologia, 2001.

Sampaio M-M., Fareleira P., Boos W. & Santos H. The substrate specificity of Glycogen Synthase from Desulfovibrio gigas could explain the lack of regulation in glycogen biosynthesis. BIOQUIMICA 2000, XII Congresso Nacional de Bioquímica, Póvoa do Varzim, 2000.

ORAL COMMUNICATIONS

Pinteus S, Monteiro H, Araujo E, Duarte L, Felicio L, Sampaio MM, Afonso C, Cruz A, Mouga T, Pedrosa R (2009). High antimicrobial potential of methanol extracts of Sphaerococcus coronopifolius and Plocamium cartilagineum collected from the Peniche Coast (Portugal). International Meeting on Marine Resources 09, Peniche, Portugal, 16-18 Novembro

Sampaio M-M. “Engineering Escherichia coli for the production of Mannosylglycerate”. ITQB. 2004. Boos W., Sampaio M-M., “Mannosylglycerate transport in E. coli”. 5th European Project Meeting “Hypersolutes”, March 2003.

Sampaio M-M. “The use of 14_{C-compounds to study osmoadaptation in thermophiles”. Gulbenkian}

Institute of Science, Oeiras, October 2003.

Sampaio M-M. “Synthesis of mannosylglycerate in engineered E. coli cells”. 3rd European Project Meeting “Hypersolutes”, March 2002.

Eppler T., Sampaio M-M., Horlacher R., Boos W. “Construction of an expression system for Bacillus stearothermophilus”. 3rd European Project Meeting “Hypersolutes”, March 2002.

Sampaio M-M. “Optimisation of MG synthesis in E. coli: in the search for a MG synthase with optimal activity in the mesophilic range of temperature”. 4th European Project Meeting “Hypersolutes”, September 2002.