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Rapid Identification of Fungi by Using the ITS2 Genetic Region and an Automated Fluorescent Capillary Electrophoresis System

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Figure

FIG. 1. Schematic representation of the fungal ribosomal genes containingthe primer target areas used in the amplification of the ITS2 region.
FIG. 2. Specificity of universal ITS2 primers against bacteria and humangenomic DNA. PCR amplification using the ITS4 and ITS86 primer pair was
TABLE 2. Length determination (in base pairs) of the PCR fragment containing the ITS2 of known fungal isolates
FIG. 4. Electropherograms of five CandidaPRISM 310 genetic analyzer. Control strains (graphs 1 to 5) were amplified byusing ITS4 and fluorescently labeled ITS86 and dUTP as described in Materialsand Methods

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