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Ultrasensitive Detection of Drug Resistant Pandemic 2009 (H1N1) Influenza A Virus by Rare Variant Sensitive High Resolution Melting Curve Analysis

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Figure

FIG. 2. Experimental steps for rare variant detection by RVS-HRM. The rare variant detection by RVS-HRM was determined on thebasis of two experimental steps
TABLE 1. Statistical probability of detecting 0.5% of rare variant on a 384-well plate
FIG. 3. Sensitivity of rare-variant detection by RVS-HRM. RVS-HRM sensitivity tests were carried out on 384-well plates in triplicate (runs1, 2, and 3)
FIG. 4. Detection of the wild-type and oseltamivir-resistant virus by real-time PCR and RVS-HRM

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