Primers: 5 visible, 5 total
10 20 30 40 50 60
5ʹ
3ʹ
g a a t t c t a t t t t c g a t t t c a a a t t t t c c a a a c t t a a a t a t g a t t g a a t g c c t g a g a a a g g t a a t a
c t t a a g a t a a a a g c t a a a g t t t a a a a g g t t t g a a t t t a t a c t a a c t t a c g g a c t c t t t c c a t t a t
g a a t t c t a t t t t c g a t t t c a a a t t t t c c a a a c t t a a a t a t g a t t g a a t g c c t g a g a a a g g t a a t a g
60 70 80 90 100 110 120 130
g a g a t g t t t t a g t t t a t t a t g a a g t a t t a g g g g c g t c t t t t a a a t t c a a t c t a t c a a t t t g t g a a
c t c t a c a a a a t c a a a t a a t a c t t c a t a a t c c c c g c a g a a a a t t t a a g t t a g a t a g t t a a a c a c t t
g t a a t a g a g a t g t t t t a g t t t a t t a t g a a g t a t t a g g g g c g t c t t t t a a a t t c a a t c t a t c a a t t t g t g a a a
130 140 150 160 170 180 190
a t a t a t t a c t c a a a a c c c a a t a c c a t t c t a a a a c t t a t t c a a a a t a t a t a t t g c t t t a a a a g a g c
t a t a t a a t g a g t t t t g g g t t a t g g t a a g a t t t t g a a t a a g t t t t a t a t a t a a c g a a a t t t t c t c g
t g t g a a a t a t a t t a c t c a a a a c c c a a t a c c a t t c t a a a a c t t a t t c a a a a t a t a t a t t g c t t t a a a a g a g c a
190 200 210 220 230 240 250 260
BspT104I Bsp119I Csp45I Bpu14I BstBI NspV AsuII SfuI
a t a c a t a c t a a a a a a a c a g g c a t c t t t c g a a c t a t a g c g c a t a g a a t a c t a c g g t g a a t c a a a a a
t a t g t a t g a t t t t t t t g t c c g t a g a a a g c t t g a t a t c g c g t a t c t t a t g a t g c c a c t t a g t t t t t
a a g a g c a t a c a t a c t a a a a a a a c a g g c a t c t t t c g a a c t a t a g c g c a t a g a a t a c t a c g g t g a a t c a a a a a c
260 270 280 290 300 310 320
c a a a t a a a a t t t a g g a g g t a t a t t c a a g t a t a c a a a a a a a c t t t a g t g t g a g g g g a t t t a g a t a a
g t t t a t t t t a a a t c c t c c a t a t a a g t t c a t a t g t t t t t t t g a a a t c a c a c t c c c c t a a a t c t a t t
390 400 410 420 430 440 450
a t g t g a a t t a a g t c t a t c a a t t t a a t t t a t t a t g t t a c t t t a t a t t t g a t t a a t a a t t g c a a g t t
t a c a c t t a a t t c a g a t a g t t a a a t t a a a t a a t a c a a t g a a a t a t a a a c t a a t t a t t a a c g t t c a a
a a t a t t a t g t g a a t t a a g t c t a t c a a t t t a a t t t a t t a t g t t a c t t t a t a t t t g a t t a a t a a t t g c a a g t t t
450 460 470 480 490 500 510 520
Eco53kI Ecl136II EcoICRI
* His Gln Phe Ala Val Ile Arg Ile Leu Lys Leu Leu Gln Lys Asn Ser Ser t a a a a t c a t a a t t t a a t g t t g a a a g g c c a c t a t t c t a a t t a a c t t a a g g a g t t g t t t a t t t g a g c
a t t t t a g t a t t a a a t t a c a a c t t t c c g g t g a t a a g a t t a a t t g a a t t c c t c a a c a a a t a a a c t c g
c a a g t t t a a a a t c a t a a t t t a a t g t t g a a a g g c c a c t a t t c t a a t t a a c t t a a g g a g t t g t t t a t t t g a g c t
520 530 540 550 560 570 580
Psp124BI EcoT38I Eco24I BanII FriOI SacI SstI
BsiHKCI Ama87I Eco88I XmaCI TspMI BsoBI XmaI Cfr9I AvaI
BmeT110I SmaI
Pro Val Arg Pro Tyr Asp Gln Phe Ser Pro His Arg Phe Val Phe Phe Met Leu Phe Met Tyr t c g g t a c c c g g g g a t a a t c t t g a a a g g a g g g a t g c c t a a a a a c g a a g a a c a t t a a a a a c a t a t a t
a g c c a t g g g c c c c t a t t a g a a c t t t c c t c c c t a c g g a t t t t t g c t t c t t g t a a t t t t t g t a t a t a
t t g a g c t c g g t a c c c g g g g a t a a t c t t g a a a g g a g g g a t g c c t a a a a a c g a a g a a c a t t a a a a a c a t a t a t t
580 590 600 610 620 630 640 650
Lys Cys Arg Arg Ile Ser Lys His Phe Ile Met Lys Asp Thr Gln Phe Asn His Ile Ile Ile Leu t t g c a c c g t c t a a t g g a t t t a t g a a a a a t c a t t t t a t c a g t t t g a a a a t t a t g t a t t a t g a t a a g
650 660 670 680 690 700 710
SwaI SmiI Bsp19I
NcoI
1 5 10 15
Met Gly Asn Leu Asn Asn Leu Gly Gly Tyr Glu Asp Ser Asn Arg Thr Cry7Aa2_MB39_NA311.1
C C A T G G G T A A T T T A A A T A A T T T A G G T G G A T A T G A A G A T A G T A A T A G T C
Cry7Aa2_Fw_NcoI
Met Gly Asn Leu Asn Asn Leu Gly Gly Tyr Glu Asp Ser Asn Arg Thr Phe Leu Leu Phe Phe Trp Pro Tyr Asn Leu Tyr Asn Leu His Ile His Leu Tyr Tyr Tyr Phe Met a a a g a g g a g g a a a a a c c a t g g g t a a t t t a a a t a a t t t a g g t g g a t a t g a a g a t a g t a a t a g a a c a
t t t c t c c t c c t t t t t g g t a c c c a t t a a a t t t a t t a a a t c c a c c t a t a c t t c t a t c a t t a t c t t g t
g a t a a g a a a g a g g a g g a a a a a c c a t g g g t a a t t t a a a t a a t t t a g g t g g a t a t g a a g a t a g t a a t a g a a c a t 1 ▬
◄
A A A G A G G A G G A A A A A C C A T G G G T A A T T T A A A T A A T T T A G G T G G A T A T G A A G A T A G T A A T A G A A C A T710 720 730 740 750 760 770 780
20 25 30 35
Leu Asn Asn Ser Leu Asn Tyr Pro Thr Gln Lys Ala Leu Ser Pro Ser Leu Lys Asp Met Asn Tyr Cry7Aa2_MB39_NA311.1
Leu Asn Asn Ser Leu Asn Tyr Pro Thr Gln Lys Ala Leu Ser Pro Ser Leu Lys Asp Met Asn Tyr t t a a a t a a t t c t c t c a a t t a t c c t a c t c a a a a a g c a t t a t c a c c a t c a t t a a a g g a t a t g a a c t a
a a t t t a t t a a g a g a g t t a a t a g g a t g a g t t t t t c g t a a t a g t g g t a g t a a t t t c c t a t a c t t g a t
a g a a c a t t a a a t a a t t c t c t c a a t t a t c c t a c t c a a a a a g c a t t a t c a c c a t c a t t a a a g g a t a t g a a c t a c 1 ▬
◄
T T A A A T A A T T C T C T C A A T T A T C C T A C T C A A A A A G C A T T A T C A C C A T C A T T A A A G G A T A T G A A C T A C780 790 800 810 820 830 840
40 45 50 55
Gln Asp Phe Leu Ser Ile Thr Glu Arg Glu Gln Pro Glu Ala Leu Ala Ser Gly Asn Thr Ala Cry7Aa2_MB39_NA311.1
Gln Asp Phe Leu Ser Ile Thr Glu Arg Glu Gln Pro Glu Ala Leu Ala Ser Gly Asn Thr Ala c c a g g a t t t t t t a t c t a t a a c t g a g a g g g a a c a a c c t g a a g c a c t c g c t a g t g g t a a t a c a g c t a
g g t c c t a a a a a a t a g a t a t t g a c t c t c c c t t g t t g g a c t t c g t g a g c g a t c a c c a t t a t g t c g a t
840 850 860 870 880 890 900 910
DraIII AdeI
60 65 70 75 80
Ile Asn Thr Val Val Ser Val Thr Gly Ala Thr Leu Ser Ala Leu Gly Val Pro Gly Ala Ser Phe Cry7Aa2_MB39_NA311.1
Ile Asn Thr Val Val Ser Val Thr Gly Ala Thr Leu Ser Ala Leu Gly Val Pro Gly Ala Ser Phe t t a a t a c t g t a g t t a g t g t t a c a g g g g c t a c a c t a a g t g c g t t a g g t g t c c c a g g t g c a a g t t t t
a a t t a t g a c a t c a a t c a c a a t g t c c c c g a t g t g a t t c a c g c a a t c c a c a g g g t c c a c g t t c a a a a
c a g c t a t t a a t a c t g t a g t t a g t g t t a c a g g g g c t a c a c t a a g t g c g t t a g g t g t c c c a g g t g c a a g t t t t a 1 ▬
◄
T T A A T A C T G T A G T T A G T G T T A C A G G G G C T A C A C T A A G T G C G T T A G G T G T C C C A G G T G C A A G T T T T A910 920 930 940 950 960 970
Msp20I MluNI MscI MlsI BalI
Eco147I SseBI StuI AatI PceI
85 90 95 100
Ile Thr Asn Phe Tyr Leu Lys Ile Ala Gly Leu Leu Trp Pro Lys Asp Lys Asn Ile Trp Asp Glu Cry7Aa2_MB39_NA311.1
Ile Thr Asn Phe Tyr Leu Lys Ile Ala Gly Leu Leu Trp Pro Lys Asp Lys Asn Ile Trp Asp Glu a t c a c t a a c t t t t a c c t g a a a a t t g c a g g c c t t t t a t g g c c a a a a g a t a a a a a t a t t t g g g a t g a
t a g t g a t t g a a a a t g g a c t t t t a a c g t c c g g a a a a t a c c g g t t t t c t a t t t t t a t a a a c c c t a c t
a g t t t t a t c a c t a a c t t t t a c c t g a a a a t t g c a g g c c t t t t a t g g c c a a a a g a t a a a a a t a t t t g g g a t g a a 1 ▬
◄
A T C A C T A A C T T T T A C C T G A A A A T T G C A G G C C T T T T A T G G C C A A A A G A T A A A A A T A T T T G G G A T G A A970 980 990 1000 1010 1020 1030 1040
105 110 115 120
Phe Met Thr Glu Val Glu Thr Leu Ile Asp Gln Lys Ile Glu Glu Tyr Val Arg Asn Lys Ala Cry7Aa2_MB39_NA311.1
Phe Met Thr Glu Val Glu Thr Leu Ile Asp Gln Lys Ile Glu Glu Tyr Val Arg Asn Lys Ala a t t t a t g a c a g a a g t a g a a a c a c t t a t t g a t c a a a a a a t a g a a g a a t a t g t a a g g a a t a a a g c g a
t a a a t a c t g t c t t c a t c t t t g t g a a t a a c t a g t t t t t t a t c t t c t t a t a c a t t c c t t a t t t c g c t
1040 1050 1060 1070 1080 1090 1100
125 130 135 140 145
Ile Ala Glu Leu Asn Gly Leu Gly Ala Ala Leu Asp Lys Tyr Gln Lys Ala Leu Ala Asp Trp Leu Cry7Aa2_MB39_NA311.1
Ile Ala Glu Leu Asn Gly Leu Gly Ala Ala Leu Asp Lys Tyr Gln Lys Ala Leu Ala Asp Trp Leu t t g c a g a a t t a a a t g g a t t a g g a g c a g c c t t a g a t a a a t a t c a a a a a g c a c t t g c a g a t t g g c t g
a a c g t c t t a a t t t a c c t a a t c c t c g t c g g a a t c t a t t t a t a g t t t t t c g t g a a c g t c t a a c c g a c
a a g c g a t t g c a g a a t t a a a t g g a t t a g g a g c a g c c t t a g a t a a a t a t c a a a a a g c a c t t g c a g a t t g g c t g g 1 ▬
◄
T T G C A G A A T T A A A T G G A T T A G G A G C A G C C T T A G A T A A A T A T C A A A A A G C A C T T G C A G A T T G G C T G G1100 1110 1120 1130 1140 1150 1160 1170
150 155 160 165
Gly Lys Gln Asp Asp Pro Ala Ala Ile Phe Ala Val Ala Thr Glu Phe Arg Ile Ile Asp Ser Leu Cry7Aa2_MB39_NA311.1
Gly Lys Gln Asp Asp Pro Ala Ala Ile Phe Ala Val Ala Thr Glu Phe Arg Ile Ile Asp Ser Leu g g c a a a c a a g a t g a t c c a g c a g c t a t a t t t g c t g t g g c a a c t g a a t t t c g t a t a a t a g a t t c t c t
c c g t t t g t t c t a c t a g g t c g t c g a t a t a a a c g a c a c c g t t g a c t t a a a g c a t a t t a t c t a a g a g a
t g g c t g g g c a a a c a a g a t g a t c c a g c a g c t a t a t t t g c t g t g g c a a c t g a a t t t c g t a t a a t a g a t t c t c t t 1 ▬
◄
G G C A A A C A A G A T G A T C C A G C A G C T A T A T T T G C T G T G G C A A C T G A A T T T C G T A T A A T A G A T T C T C T T1170 1180 1190 1200 1210 1220 1230
170 175 180 185
Phe Glu Phe Ser Met Pro Ser Phe Lys Val Thr Gly Tyr Glu Ile Pro Leu Leu Thr Val Tyr Cry7Aa2_MB39_NA311.1
Phe Glu Phe Ser Met Pro Ser Phe Lys Val Thr Gly Tyr Glu Ile Pro Leu Leu Thr Val Tyr t t t t g a a t t t a g t a t g c c t t c a t t t a a g g t t a c t g g a t a t g a a a t a c c a t t a c t a a c a g t t t a c g
a a a a c t t a a a t c a t a c g g a a g t a a a t t c c a a t g a c c t a t a c t t t a t g g t a a t g a t t g t c a a a t g c
t t c t c t t t t t g a a t t t a g t a t g c c t t c a t t t a a g g t t a c t g g a t a t g a a a t a c c a t t a c t a a c a g t t t a c g c 1 ▬
◄
T T T T G A A T T T A G T A T G C C T T C A T T T A A G G T T A C T G G A T A T G A A A T A C C A T T A C T A A C A G T T T A C G C1230 1240 1250 1260 1270 1280 1290 1300
190 195 200 205 210
Ala Gln Ala Ala Asn Leu His Leu Ala Leu Leu Arg Asp Ser Thr Leu Tyr Gly Asp Lys Trp Gly Cry7Aa2_MB39_NA311.1
c a c a a g c g g c a a a c c t t c a t c t a g c t t t a t t a a g a g a t t c t a c t c t t t a t g g a g a t a a a t g g g g a
1300 1310 1320 1330 1340 1350 1360
215 220 225 230
Phe Thr Gln Asn Asn Ile Glu Glu Asn Tyr Asn Arg Gln Lys Lys His Ile Ser Glu Tyr Ser Gly Cry7Aa2_MB39_NA311.1
Phe Thr Gln Asn Asn Ile Glu Glu Asn Tyr Asn Arg Gln Lys Lys His Ile Ser Glu Tyr Ser Gly t t c a c t c a g a a c a a c a t t g a g g a a a a t t a t a a t c g t c a a a a g a a a c a t a t t t c t g a a t a t t c a g g
a a g t g a g t c t t g t t g t a a c t c c t t t t a a t a t t a g c a g t t t t c t t t g t a t a a a g a c t t a t a a g t c c
t g g g g a t t c a c t c a g a a c a a c a t t g a g g a a a a t t a t a a t c g t c a a a a g a a a c a t a t t t c t g a a t a t t c a g g c 1 ▬
◄
T T C A C T C A G A A C A A C A T T G A G G A A A A T T A T A A T C G T C A A A A G A A A C A T A T T T C T G A A T A T T C A G G C1360 1370 1380 1390 1400 1410 1420 1430
235 240 245 250
His Cys Thr Glu Trp Tyr Asn Ser Gly Leu Ser Arg Leu Asn Gly Ser Thr Tyr Glu Gln Trp Cry7Aa2_MB39_NA311.1
His Cys Thr Glu Trp Tyr Asn Ser Gly Leu Ser Arg Leu Asn Gly Ser Thr Tyr Glu Gln Trp c c a t t g t a c c g a g t g g t a t a a t a g t g g t c t t a g c a g a t t g a a c g g t t c c a c t t a t g a a c a a t g g a
g g t a a c a t g g c t c a c c a t a t t a t c a c c a g a a t c g t c t a a c t t g c c a a g g t g a a t a c t t g t t a c c t
t t c a g g c c a t t g t a c c g a g t g g t a t a a t a g t g g t c t t a g c a g a t t g a a c g g t t c c a c t t a t g a a c a a t g g a t 1 ▬
◄
C C A T T G T A C C G A G T G G T A T A A T A G T G G T C T T A G C A G A T T G A A C G G T T C C A C T T A T G A A C A A T G G A T1430 1440 1450 1460 1470 1480 1490
BglII
255 260 265 270 275
Ile Asn Tyr Asn Arg Phe Arg Arg Glu Met Ile Leu Met Val Leu Asp Leu Val Ala Val Phe Pro Cry7Aa2_MB39_NA311.1
Ile Asn Tyr Asn Arg Phe Arg Arg Glu Met Ile Leu Met Val Leu Asp Leu Val Ala Val Phe Pro t a a a t t a t a a t c g t t t t c g t a g a g a a a t g a t a t t a a t g g t a t t a g a t c t t g t c g c t g t a t t t c c t
a t t t a a t a t t a g c a a a a g c a t c t c t t t a c t a t a a t t a c c a t a a t c t a g a a c a g c g a c a t a a a g g a
a a t g g a t a a a t t a t a a t c g t t t t c g t a g a g a a a t g a t a t t a a t g g t a t t a g a t c t t g t c g c t g t a t t t c c t a 1 ▬
◄
T A A A T T A T A A T C G T T T T C G T A G A G A A A T G A T A T T A A T G G T A T T A G A T C T T G T C G C T G T A T T T C C T A1490 1500 1510 1520 1530 1540 1550 1560
1560 1570 1580 1590 1600 1610 1620
300 305 310 315
Pro Val Ile Leu Ser Ile Ser Asn Pro Asp Ile Gly Pro Ser Phe Ser Gln Met Glu Asn Thr Cry7Aa2_MB39_NA311.1
C A A T T A G C A A T C C A G A T A T A G G Cry7Aa2-seq-Fw
Pro Val Ile Leu Ser Ile Ser Asn Pro Asp Ile Gly Pro Ser Phe Ser Gln Met Glu Asn Thr t c c a g t t a t c t t g t c a a t t a g c a a t c c a g a t a t a g g t c c a a g t t t t t c t c a g a t g g a a a a t a c t g
a g g t c a a t a g a a c a g t t a a t c g t t a g g t c t a t a t c c a g g t t c a a a a a g a g t c t a c c t t t t a t g a c
t a c c g a t c c a g t t a t c t t g t c a a t t a g c a a t c c a g a t a t a g g t c c a a g t t t t t c t c a g a t g g a a a a t a c t g c 1 ▬
◄
T C C A G T T A T C T T G T C A A T T A G C A A T C C A G A T A T A G G T C C A A G T T T T T C T C A G A T G G A A A A T A C T G C 3 ▬◄
A A‒T A C T G C1620 1630 1640 1650 1660 1670 1680 1690
320 325 330 335 340
Ala Ile Arg Thr Pro His Leu Val Asp Tyr Leu Asp Glu Leu Tyr Ile Tyr Thr Ser Lys Tyr Lys Cry7Aa2_MB39_NA311.1
Ala Ile Arg Thr Pro His Leu Val Asp Tyr Leu Asp Glu Leu Tyr Ile Tyr Thr Ser Lys Tyr Lys c g a t t a g a a c a c c a c a c c t t g t t g a t t a t t t a g a t g a g c t t t a t a t a t a t a c a t c a a a a t a t a a a
g c t a a t c t t g t g g t g t g g a a c a a c t a a t a a a t c t a c t c g a a a t a t a t a t a t g t a g t t t t a t a t t t
a t a c t g c g a t t a g a a c a c c a c a c c t t g t t g a t t a t t t a g a t g a g c t t t a t a t a t a t a c a t c a a a a t a t a a a g 1 ▬
◄
C G A T T A G A A C A C C A C A C C T T G T T G A T T A T T T A G A T G A G C T T T A T A T A T A T A C A T C A A A A T A T A A A G 3 ▬◄
C G A T T A G A A C A C C A C A C C T T G T T G A T T A T T T A G A T G A G C T T T A T A T A T A T A C A T C A A A A T A T A A A G1690 1700 1710 1720 1730 1740 1750
345 350 355 360
Ala Phe Ser His Glu Ile Gln Pro Asp Leu Phe Tyr Trp Ser Ala His Lys Val Ser Phe Lys Lys Cry7Aa2_MB39_NA311.1
Ala Phe Ser His Glu Ile Gln Pro Asp Leu Phe Tyr Trp Ser Ala His Lys Val Ser Phe Lys Lys g c a t t t t c a c a t g a g a t t c a a c c a g a c c t a t t t t a t t g g a g t g c a c a t a a g g t t a g c t t t a a a a a
c g t a a a a g t g t a c t c t a a g t t g g t c t g g a t a a a a t a a c c t c a c g t g t a t t c c a a t c g a a a t t t t t
1750 1760 1770 1780 1790 1800 1810
365 370 375 380
Ser Glu Gln Ser Asn Leu Tyr Thr Thr Gly Ile Tyr Gly Lys Thr Ser Gly Tyr Ile Ser Cry7Aa2_MB39_NA311.1
Ser Glu Gln Ser Asn Leu Tyr Thr Thr Gly Ile Tyr Gly Lys Thr Ser Gly Tyr Ile Ser a t c g g a g c a a t c c a a t t t a t a t a c a a c a g g c a t a t a t g g t a a a a c a a g t g g a t a t a t t t c a t
t a g c c t c g t t a g g t t a a a t a t a t g t t g t c c g t a t a t a c c a t t t t g t t c a c c t a t a t a a a g t a
t a a a a a a t c g g a g c a a t c c a a t t t a t a t a c a a c a g g c a t a t a t g g t a a a a c a a g t g g a t a t a t t t c a t c 1 ▬
◄
A T C G G A G C A A T C C A A T T T A T A T A C A A C A G G G C A T A T A T G G G T A A A A C A A G T G G G A T T T A T T T C A T C 3 ▬◄
A T C G G A G C A A T C C A A T T T A T A T A C A A C A G G C A T A T A T G G T A A A A C A A G T G G A T A T A T T T C A T C1820 1830 1840 1850 1860 1870
385 390 395 400
Ser Gly Ala Tyr Ser Phe Arg Gly Asn Asp Ile Tyr Arg Thr Leu Ala Ala Pro Ser Val Val Cry7Aa2_MB39_NA311.1
Ser Gly Ala Tyr Ser Phe Arg Gly Asn Asp Ile Tyr Arg Thr Leu Ala Ala Pro Ser Val Val c a g g a g c a t a t t c a t t t a g a g g g a a t g a t a t c t a t a g a a c a t t a g c a g c t c c a t c a g t t g t
g t c c t c g t a t a a g t a a a t c t c c c t t a c t a t a g a t a t c t t g t a a t c g t c g a g g t a g t c a a c a
t t t c a t c a g g a g c a t a t t c a t t t a g a g g g a a t g a t a t c t a t a g a a c a t t a g c a g c t c c a t c a g t t g t a 1 ▬
◄
C A G G A G C A T A T T C A T T T T A G A G G G A A T G G A A T C C T A T A G A A C A T T T G C A G C T C C C C T C A G T T G G A A 3 ▬◄
C A G G A G C A T A T T C A T T T A G A G G G A A T G A T A T C T A T A G A A C A T T A G C A G C T C C A T C A G T T G T A1880 1890 1900 1910 1920 1930
405 410 415 420
Val Tyr Pro Tyr Thr Gln Asn Tyr Gly Val Glu Gln Val Glu Phe Tyr Gly Val Lys Gly Cry7Aa2_MB39_NA311.1
Val Tyr Pro Tyr Thr Gln Asn Tyr Gly Val Glu Gln Val Glu Phe Tyr Gly Val Lys Gly a g t t t a t c c g t a t a c t c a g a a t t a t g g t g t c g a g c a a g t t g a g t t t t a c g g t g t a a a a g g
t c a a a t a g g c a t a t g a g t c t t a a t a c c a c a g c t c g t t c a a c t c a a a a t g c c a c a t t t t c c
g t t g t a g t t t a t c c g t a t a c t c a g a a t t a t g g t g t c g a g c a a g t t g a g t t t t a c g g t g t a a a a g g a 1 ▬
◄
A G T T T A T C C G G T A A A C T C C A A A A T T T T G G G G G G C C A A G C A A G T T T G A G T T T T A C G G G 3 ▬◄
A G T T T A T C C G T A T A C T C A G A A T T A T G G T G T C G A G C A A G T T G A G T T T T A C G G T G T A A A A G G A2000 2010 2020 2030 2040 2050 2060
BsuTUI Bsu15I Bsa29I BspDI BspXI BseCI BanIII BshVI ClaI
450 455 460 465
Pro Asp Gly Glu Pro Ile His Glu Lys Tyr Thr His Arg Leu Cys His Ala Thr Ala Ile Ser Lys Cry7Aa2_MB39_NA311.1
Pro Asp Gly Glu Pro Ile His Glu Lys Tyr Thr His Arg Leu Cys His Ala Thr Ala Ile Ser Lys c a g a c g g a g a a c c a a t a c a c g a a a a a t a c a c t c a t c g a t t a t g t c a t g c t a c a g c t a t a t c t a a a
g t c t g c c t c t t g g t t a t g t g c t t t t t a t g t g a g t a g c t a a t a c a g t a c g a t g t c g a t a t a g a t t t
t a c c c c c a g a c g g a g a a c c a a t a c a c g a a a a a t a c a c t c a t c g a t t a t g t c a t g c t a c a g c t a t a t c t a a a t 3 ▬
◄
C A G A C G G A G A A C C A A T A C A C G A A A A A T A C A C T C A T C G A T T A T G T C A T G C T A C A G C T A T A T C T A A A T2070 2080 2090 2100 2110 2120 2130
470 475 480 485
Ser Thr Pro Asp Tyr Asp Asn Ala Thr Ile Pro Ile Phe Ser Trp Thr His Arg Ser Ala Glu Tyr Cry7Aa2_MB39_NA311.1
Ser Thr Pro Asp Tyr Asp Asn Ala Thr Ile Pro Ile Phe Ser Trp Thr His Arg Ser Ala Glu Tyr t c a a c t c c g g a t t a t g a t a a t g c t a c t a t c c c g a t c t t t t c t t g g a c g c a t a g a a g t g c g g a g t a
a g t t g a g g c c t a a t a c t a t t a c g a t g a t a g g g c t a g a a a a g a a c c t g c g t a t c t t c a c g c c t c a t
t c t a a a t c a a c t c c g g a t t a t g a t a a t g c t a c t a t c c c g a t c t t t t c t t g g a c g c a t a g a a g t g c g g a g t a t 3 ▬
◄
T C A A C T C C G G A T T A T G A T A A T G C T A C T A T C C C G A T C T T T T C T T G G A C G C A T A G A A G T G C G G A G T A T 4◄
▬ T C A A T T C C G G A T T A T G A T A A T G C T A C T T T C C C G A T C T T T T C T T G G A C G C A T A G A A G T G C G G A G T A T2130 2140 2150 2160 2170 2180 2190
490 495 500 505 510
Tyr Asn Arg Ile Tyr Pro Asn Lys Ile Thr Lys Ile Pro Ala Val Lys Met Tyr Lys Leu Asp Cry7Aa2_MB39_NA311.1
Tyr Asn Arg Ile Tyr Pro Asn Lys Ile Thr Lys Ile Pro Ala Val Lys Met Tyr Lys Leu Asp t t a c a a t a g a a t c t a t c c a a a c a a a a t c a c a a a a a t t c c a g c t g t a a a a a t g t a t a a a c t a g a t g
a a t g t t a t c t t a g a t a g g t t t g t t t t a g t g t t t t t a a g g t c g a c a t t t t t a c a t a t t t g a t c t a c
2200 2210 2220 2230 2240 2250 2260
515 520 525 530
Asp Pro Ser Thr Val Val Lys Gly Pro Gly Phe Thr Gly Gly Asp Leu Val Lys Arg Gly Ser Asn Cry7Aa2_MB39_NA311.1
Asp Pro Ser Thr Val Val Lys Gly Pro Gly Phe Thr Gly Gly Asp Leu Val Lys Arg Gly Ser Asn a t c c a t c t a c a g t t g t c a a a g g g c c t g g a t t t a c a g g t g g a g a t t t a g t t a a g a g a g g g a g t a a t
t a g g t a g a t g t c a a c a g t t t c c c g g a c c t a a a t g t c c a c c t c t a a a t c a a t t c t c t c c c t c a t t a
t a g a t g a t c c a t c t a c a g t t g t c a a a g g g c c t g g a t t t a c a g g t g g a g a t t t a g t t a a g a g a g g g a g t a a t g 3 ▬
◄
A T C C A T C T A C A G T T G T C A A A G G G C C T G G A T T T A C A G G T G G A G A T T T A G T T A A G A G A G G G A G T A A T G 4◄
▬ A T C C A T C T A C A G T T G T C A A A G G G C C T G G A T T T A C A G G T G G A G A T T T A G T T A A G A G A G G G A G T A A T G2260 2270 2280 2290 2300 2310 2320
535 540 545 550
Gly Tyr Ile Gly Asp Ile Lys Ala Thr Val Asn Ser Pro Leu Ser Gln Asn Tyr Arg Val Arg Val Cry7Aa2_MB39_NA311.1
Gly Tyr Ile Gly Asp Ile Lys Ala Thr Val Asn Ser Pro Leu Ser Gln Asn Tyr Arg Val Arg Val g g t t a t a t a g g a g a t a t a a a g g c t a c c g t a a a c t c a c c a c t t t c t c a a a a t t a t c g t g t t a g a g t
c c a a t a t a t c c t c t a t a t t t c c g a t g g c a t t t g a g t g g t g a a a g a g t t t t a a t a g c a c a a t c t c a
a g t a a t g g t t a t a t a g g a g a t a t a a a g g c t a c c g t a a a c t c a c c a c t t t c t c a a a a t t a t c g t g t t a g a g t t 3 ▬
◄
G G T T A T A T A G G A G A T A T A A A G G C T A C C G T A A A C T C A C C A C T T T C T C A A A A T T A T C G T G T T A G A G T T 4◄
▬ G G T T A T A T A G G A G A T A T A A A G G C T A C C G T A A A C T C A C C A C T T T C T C A A A A T T A T C G T G T T A G A G T T2330 2340 2350 2360 2370 2380 2390
555 560 565 570 575
Arg Tyr Ala Thr Asn Val Ser Gly Gln Phe Asn Val Tyr Ile Asn Asp Lys Ala Thr Leu Leu Cry7Aa2_MB39_NA311.1
Arg Tyr Ala Thr Asn Val Ser Gly Gln Phe Asn Val Tyr Ile Asn Asp Lys Ala Thr Leu Leu t c g a t a c g c c a c t a a t g t t t c t g g a c a a t t c a a c g t g t a t a t t a a t g a t a a a g c a a c g c t t c t a a
a g c t a t g c g g t g a t t a c a a a g a c c t g t t a a g t t g c a c a t a t a a t t a c t a t t t c g t t g c g a a g a t t
2390 2400 2410 2420 2430 2440 2450
580 585 590 595
Arg Lys Phe Gln Asn Thr Val Glu Thr Ile Gly Glu Gly Lys Asp Leu Thr Tyr Asp Ser Phe Gly Cry7Aa2_MB39_NA311.1
Arg Lys Phe Gln Asn Thr Val Glu Thr Ile Gly Glu Gly Lys Asp Leu Thr Tyr Asp Ser Phe Gly g a a a g t t t c a a a a t a c t g t a g a a a c a a t a g g t g a a g g a a a a g a t t t a a c c t a t g a t t c a t t t g g a
c t t t c a a a g t t t t a t g a c a t c t t t g t t a t c c a c t t c c t t t t c t a a a t t g g a t a c t a a g t a a a c c t
t t c t a a g a a a g t t t c a a a a t a c t g t a g a a a c a a t a g g t g a a g g a a a a g a t t t a a c c t a t g a t t c a t t t g g a t 3 ▬
◄
G A A A G T T T C A A A A T A C T G T A G A A A C A A T A G G T G A A G G A A A A G A T T T A A C C T A T G A T T C A T T T G G A T 4◄
▬ G A A A G T T T C A A A A T A C T G T A G A A A C A A T A G G T G A A G G A A A A G A T T T A A C C T A T G A T T C A T T T G G A T2460 2470 2480 2490 2500 2510 2520
600 605 610 615
Tyr Ile Glu Tyr Ser Thr Thr Ile Gln Phe Pro Asp Glu His Pro Lys Ile Thr Leu His Leu Ser Cry7Aa2_MB39_NA311.1
Tyr Ile Glu Tyr Ser Thr Thr Ile Gln Phe Pro Asp Glu His Pro Lys Ile Thr Leu His Leu Ser t a t a t a g a a t a t t c t a c g a c c a t t c a a t t t c c g g a t g a g c a t c c a a a a a t c a c t c t t c a t t t a a g
a t a t a t c t t a t a a g a t g c t g g t a a g t t a a a g g c c t a c t c g t a g g t t t t t a g t g a g a a g t a a a t t c
t t t g g a t a t a t a g a a t a t t c t a c g a c c a t t c a a t t t c c g g a t g a g c a t c c a a a a a t c a c t c t t c a t t t a a g t 3 ▬
◄
T A T A T A G A A T A T T C T A C G A C C A T T C A A T T T C C G G A T G A G C A T C C A A A A A T C A C T C T T C A T T T A A G T 4◄
▬ T A T A T A G A A T A T T C T A C G A C C A T T C A A T T T C C G G A T G A G C A T C C A A A A A T C A C T C T T C A T T T A A G T2520 2530 2540 2550 2560 2570 2580
620 625 630 635 640
Asp Leu Ser Asn Asn Ser Ser Phe Tyr Val Asp Ser Ile Glu Phe Ile Pro Val Asp Val Asn Cry7Aa2_MB39_NA311.1
Asp Leu Ser Asn Asn Ser Ser Phe Tyr Val Asp Ser Ile Glu Phe Ile Pro Val Asp Val Asn t g a t t t g a g t a a c a a t t c a t c a t t t t a t g t a g a t t c a a t c g a a t t t a t c c c t g t a g a t g t a a a t t
a c t a a a c t c a t t g t t a a g t a g t a a a a t a c a t c t a a g t t a g c t t a a a t a g g g a c a t c t a c a t t t a a
2590 2600 2610 2620 2630 2640 2650
645 650 655 660
Tyr Asp Glu Lys Glu Lys Leu Glu Lys Ala Gln Lys Ala Val Asn Thr Leu Phe Thr Glu Gly Arg Cry7Aa2_MB39_NA311.1
Tyr Asp Glu Lys Glu Lys Leu Glu Lys Ala Gln Lys Ala Val Asn Thr Leu Phe Thr Glu Gly Arg a t g a t g a a a a a g a a a a a c t a g a a a a a g c a c a g a a a g c c g t g a a t a c c t t g t t t a c a g a g g g a a g a
t a c t a c t t t t t c t t t t t g a t c t t t t t c g t g t c t t t c g g c a c t t a t g g a a c a a a t g t c t c c c t t c t
t a a a t t a t g a t g a a a a a g a a a a a c t a g a a a a a g c a c a g a a a g c c g t g a a t a c c t t g t t t a c a g a g g g a a g a a 3 ▬
◄
A T G A T G A A A A A G A A A A A C T A G A A A A A G C A C A G A A A G C C G T G A A T A C C T T G T T T A C A G A G G G A A G A A 4◄
▬ A T G A T G A A A A A G A A A A A C T A G A A A A A G C A C A G A A A G C C G T G A A T A C C T T G T T T A C A G A G G G A A G A A2650 2660 2670 2680 2690 2700 2710
SexAI* MabI CsiI
665 670 675 680
Asn Ala Leu Gln Lys Asp Val Thr Asp Tyr Lys Val Asp Gln Val Ser Ile Leu Val Asp Cys Ile Cry7Aa2_MB39_NA311.1
Asn Ala Leu Gln Lys Asp Val Thr Asp Tyr Lys Val Asp Gln Val Ser Ile Leu Val Asp Cys Ile a a t g c a c t c c a a a a a g a c g t g a c a g a t t a t a a a g t g g a c c a g g t t t c a a t t t t a g t g g a t t g t a t
t t a c g t g a g g t t t t t c t g c a c t g t c t a a t a t t t c a c c t g g t c c a a a g t t a a a a t c a c c t a a c a t a
g g a a g a a a t g c a c t c c a a a a a g a c g t g a c a g a t t a t a a a g t g g a c c a g g t t t c a a t t t t a g t g g a t t g t a t a 3 ▬
◄
A A T G C A C T C C A A A A A G A C G T G A C A G A T T A T A A A G T G G A C C A G G T T T C A A T T T T A G T G G A T T G T A T A 4◄
▬ A A T G C A C T C C A A A A A G A C G T G A C A G A T T A T A A A G T G G A C C A G G T T T C A A T T T T A G T G G A T T G T A T A2720 2730 2740 2750 2760 2770 2780
685 690 695 700 705
Ser Gly Asp Leu Tyr Pro Asn Glu Lys Arg Glu Leu Gln Asn Leu Val Lys Tyr Ala Lys Arg Cry7Aa2_MB39_NA311.1
Ser Gly Asp Leu Tyr Pro Asn Glu Lys Arg Glu Leu Gln Asn Leu Val Lys Tyr Ala Lys Arg a t c a g g g g a t t t a t a t c c c a a t g a g a a a c g c g a a c t a c a a a a t c t a g t c a a a t a c g c a a a a c g t t
t a g t c c c c t a a a t a t a g g g t t a c t c t t t g c g c t t g a t g t t t t a g a t c a g t t t a t g c g t t t t g c a a
2780 2790 2800 2810 2820 2830 2840
710 715 720 725
Leu Ser Tyr Ser Arg Asn Leu Leu Leu Asp Pro Thr Phe Asp Ser Ile Asn Ser Ser Glu Glu Asn Cry7Aa2_MB39_NA311.1
Leu Ser Tyr Ser Arg Asn Leu Leu Leu Asp Pro Thr Phe Asp Ser Ile Asn Ser Ser Glu Glu Asn t g a g c t a t t c c c g t a a t t t a c t t c t a g a t c c a a c a t t c g a t t c t a t t a a t t c a t c t g a g g a g a a t
a c t c g a t a a g g g c a t t a a a t g a a g a t c t a g g t t g t a a g c t a a g a t a a t t a a g t a g a c t c c t c t t a
a a c g t t t g a g c t a t t c c c g t a a t t t a c t t c t a g a t c c a a c a t t c g a t t c t a t t a a t t c a t c t g a g g a g a a t g 3 ▬
◄
T G A G C T A T T C C C G T A A T T T A C T T C T A G A T C C A‒C A T T C G A T T C‒A T T A A T T C A T C T G A G G A A A A A G 4◄
▬ T G A G C T A T T C C C G T A A T T T A C T T C T A G A T C C A A C A T T C G A T T C T A T T A A T T C A T C T G A G G A G A A T G2850 2860 2870 2880 2890 2900 2910
730 735 740 745
Gly Trp Tyr Gly Ser Asn Gly Ile Val Ile Gly Asn Gly Asp Phe Val Phe Lys Gly Asn Tyr Leu Cry7Aa2_MB39_NA311.1
Gly Trp Tyr Gly Ser Asn Gly Ile Val Ile Gly Asn Gly Asp Phe Val Phe Lys Gly Asn Tyr Leu g g t t g g t a t g g a a g t a a t g g t a t t g t g a t t g g a a a t g g g g a t t t t g t a t t c a a a g g t a a c t a t t t
c c a a c c a t a c c t t c a t t a c c a t a a c a c t a a c c t t t a c c c c t a a a a c a t a a g t t t c c a t t g a t a a a
g a g a a t g g t t g g t a t g g a a g t a a t g g t a t t g t g a t t g g a a a t g g g g a t t t t g t a t t c a a a g g t a a c t a t t t a 3 ▬
◄
G G T T G G A A T G G A A A T A A T G G T A T T G G A A T T G G A A A G G G G G A T T T T T T‒T T C C A A G 4◄
▬ G G T T G G T A T G G A A G T A A T G G T A T T G T G A T T G G A A A T G G G G A T T T T G T A T T C A A A G G T A A C T A T T T A2910 2920 2930 2940 2950 2960 2970
750 755 760 765 770
Ile Phe Ser Gly Thr Asn Asp Thr Gln Tyr Pro Thr Tyr Leu Tyr Gln Lys Ile Asp Glu Ser Cry7Aa2_MB39_NA311.1
Ile Phe Ser Gly Thr Asn Asp Thr Gln Tyr Pro Thr Tyr Leu Tyr Gln Lys Ile Asp Glu Ser a a t t t t t t c a g g t a c c a a t g a t a c a c a a t a t c c a a c a t a t c t c t a c c a a a a a a t a g a t g a a t c c a
t t a a a a a a g t c c a t g g t t a c t a t g t g t t a t a g g t t g t a t a g a g a t g g t t t t t t a t c t a c t t a g g t
2980 2990 3000 3010 3020 3030
775 780 785
Lys Leu Lys Glu Tyr Thr Arg Tyr Lys Leu Lys Gly Phe Ile Glu Ser Ser Gln Asp Cry7Aa2_MB39_NA311.1
Lys Leu Lys Glu Tyr Thr Arg Tyr Lys Leu Lys Gly Phe Ile Glu Ser Ser Gln Asp a a c t c a a a g a a t a t a c a c g c t a t a a a c t g a a a g g t t t t a t c g a a a g t a g t c a g g a t t
t t g a g t t t c t t a t a t g t g c g a t a t t t g a c t t t c c a a a a t a g c t t t c a t c a g t c c t a a
a a t c c a a a c t c a a a g a a t a t a c a c g c t a t a a a c t g a a a g g t t t t a t c g a a a g t a g t c a g g a t t t 2
◄
▬ A A C T C A A A G A A A T T T C C C C‒ ‒ ‒ ‒ ‒ ‒C G T T A A A A A C T G A A A G G T T T T T A C G A A A G T A G T C A G G A T T T 4◄
▬ A A C T C A A A G A A T A T A C A C G C T A T A A A C T G A A A G G T T T T A T C G A A A G T A G T C A G G A T T T3030 3040 3050 3060 3070 3080 3090 3100
790 795 800 805 810
Leu Glu Ala Tyr Val Ile Arg Tyr Asp Ala Lys His Arg Thr Leu Asp Val Ser Asp Asn Leu Leu Cry7Aa2_MB39_NA311.1
Leu Glu Ala Tyr Val Ile Arg Tyr Asp Ala Lys His Arg Thr Leu Asp Val Ser Asp Asn Leu Leu t a g a a g c t t a t g t g a t t c g c t a t g a t g c a a a a c a t a g a a c a t t g g a t g t t t c t g a t a a t c t a t t
a t c t t c g a a t a c a c t a a g c g a t a c t a c g t t t t g t a t c t t g t a a c c t a c a a a g a c t a t t a g a t a a
a g g a t t t a g a a g c t t a t g t g a t t c g c t a t g a t g c a a a a c a t a g a a c a t t g g a t g t t t c t g a t a a t c t a t t a 2
◄
▬ T A G A A G C T T A T G T G A T T C G C T A T G A T G C A A A A C A T A G A A C C A T T G G A T G T T T C T G A T A A T C T A T T A 4◄
▬ T A G A A G C T T A T G T G A T T C G C T A T G A T G C A A A A C A T A G A A C A T T G G A T G T T T C T G A T A A T C T A T T A3100 3110 3120 3130 3140 3150 3160
815 820 825 830
Pro Asp Ile Leu Pro Glu Asn Thr Cys Gly Glu Pro Asn Arg Cys Ala Ala Gln Gln Tyr Leu Cry7Aa2_MB39_NA311.1
Pro Asp Ile Leu Pro Glu Asn Thr Cys Gly Glu Pro Asn Arg Cys Ala Ala Gln Gln Tyr Leu a c c g g a t a t t c t c c c t g a g a a t a c a t g t g g a g a a c c a a a t c g c t g c g c g g c a c a a c a a t a c c t
t g g c c t a t a a g a g g g a c t c t t a t g t a c a c c t c t t g g t t t a g c g a c g c g c c g t g t t g t t a t g g a
3160 3170 3180 3190 3200 3210 3220
835 840 845 850
Asp Glu Asn Pro Ser Ser Glu Cys Ser Ser Met Gln Asp Gly Ile Leu Ser Asp Ser His Ser Cry7Aa2_MB39_NA311.1
Asp Glu Asn Pro Ser Ser Glu Cys Ser Ser Met Gln Asp Gly Ile Leu Ser Asp Ser His Ser g g a t g a a a a t c c a a g t t c a g a a t g t a g t t c g a t g c a a g a t g g a a t t t t g t c t g a t t c g c a t t c a t
c c t a c t t t t a g g t t c a a g t c t t a c a t c a a g c t a c g t t c t a c c t t a a a a c a g a c t a a g c g t a a g t a
t a c c t g g a t g a a a a t c c a a g t t c a g a a t g t a g t t c g a t g c a a g a t g g a a t t t t g t c t g a t t c g c a t t c a t t 2
◄
▬ G G A T G A A A A T C C A A G T T C A G A A T G T A G T T C G A T G C A A G A T G G A A T T T T G T C T G A T T C G C A T T C A T T 4◄
▬ G G A T G A A A A T C C A A G T T C A G A A T G T A G T T C G A T G C A A G A T G G A A T T T T G T C T G A T T C G C A T T C A T T3230 3240 3250 3260 3270 3280 3290
855 860 865 870 875
Phe Ser Leu Asn Ile Asp Thr Gly Ser Ile Asn His Asn Glu Asn Leu Gly Ile Trp Val Leu Phe Cry7Aa2_MB39_NA311.1
Phe Ser Leu Asn Ile Asp Thr Gly Ser Ile Asn His Asn Glu Asn Leu Gly Ile Trp Val Leu Phe t t t c t c t t a a t a t a g a t a c a g g t t c t a t c a a t c a c a a t g a g a a t t t a g g a a t t t g g g t g t t g t t t
a a a g a g a a t t a t a t c t a t g t c c a a g a t a g t t a g t g t t a c t c t t a a a t c c t t a a a c c c a c a a c a a a
a t t c a t t t t c t c t t a a t a t a g a t a c a g g t t c t a t c a a t c a c a a t g a g a a t t t a g g a a t t t g g g t g t t g t t t a 2
◄
▬ T T T C T C T T A A T A T A G A T A C A G G T T C T A T C A A T C A C A A T G A G A A T T T A G G A A T T T G G G T G T T G T T T A 4◄
▬ T T T C T C T T A A T A T A G A T A C A G G T T C T A T C A A T C A C A A T G A G A A T T T A G G A A T T T G G G3290 3300 3310 3320 3330 3340 3350
880 885 890 895
Lys Ile Ser Thr Leu Glu Gly Tyr Ala Lys Phe Gly Asn Leu Glu Val Ile Glu Asp Gly Pro Val Cry7Aa2_MB39_NA311.1
G C C C A G T TestCry7Aa2 (David)
g c t g t a a t c t t c c t a t a c g c Cry7Aa2-seq-Rv
Lys Ile Ser Thr Leu Glu Gly Tyr Ala Lys Phe Gly Asn Leu Glu Val Ile Glu Asp Gly Pro Val a a a a t t t c g a c a t t a g a a g g a t a t g c g a a a t t t g g a a a t c t a g a a g t g a t t g a a g a t g g c c c a g t
t t t t a a a g c t g t a a t c t t c c t a t a c g c t t t a a a c c t t t a g a t c t t c a c t a a c t t c t a c c g g g t c a
3360 3370 3380 3390 3400 3410 3420
Van91I AccB7I PflMI
900 905 910 915
Ile Gly Glu Ala Leu Ala Arg Val Lys Arg Gln Glu Thr Lys Trp Arg Asn Lys Leu Ala Gln Cry7Aa2_MB39_NA311.1
T A T T G G A G A A G C A T T A G C TestCry7Aa2 (David)
Ile Gly Glu Ala Leu Ala Arg Val Lys Arg Gln Glu Thr Lys Trp Arg Asn Lys Leu Ala Gln t a t t g g a g a a g c a t t a g c c c g t g t g a a a c g c c a a g a a a c g a a g t g g a g a a a c a a g t t a g c c c a a c
a t a a c c t c t t c g t a a t c g g g c a c a c t t t g c g g t t c t t t g c t t c a c c t c t t t g t t c a a t c g g g t t g
c c c a g t t a t t g g a g a a g c a t t a g c c c g t g t g a a a c g c c a a g a a a c g a a g t g g a g a a a c a a g t t a g c c c a a c t 2
◄
▬ T A T T G G A G A A G C A T T A G C C C G T G T G A A A C G C C A A G A A A C G A A G T G G A G A A A C A A G T T A G C C C A A C T3420 3430 3440 3450 3460 3470 3480
920 925 930 935 940
Leu Thr Thr Glu Thr Gln Ala Ile Tyr Thr Arg Ala Lys Gln Ala Leu Asp Asn Leu Phe Ala Asn Cry7Aa2_MB39_NA311.1
Leu Thr Thr Glu Thr Gln Ala Ile Tyr Thr Arg Ala Lys Gln Ala Leu Asp Asn Leu Phe Ala Asn t g a c a a c g g a a a c a c a a g c g a t t t a t a c a c g a g c a a a a c a a g c g c t g g a t a a t c t t t t t g c g a a t
a c t g t t g c c t t t g t g t t c g c t a a a t a t g t g c t c g t t t t g t t c g c g a c c t a t t a g a a a a a c g c t t a
c c c a a c t g a c a a c g g a a a c a c a a g c g a t t t a t a c a c g a g c a a a a c a a g c g c t g g a t a a t c t t t t t g c g a a t g 2
◄
▬ T G A C A A C G G A A A C A C A A G C G A T T T A T A C A C G A G C A A A A C A A G C G C T G G A T A A T C T T T T T G C G A A T G3490 3500 3510 3520 3530 3540 3550
945 950 955 960
Ala Gln Asp Ser His Leu Lys Arg Asp Val Thr Phe Ala Glu Ile Ala Ala Ala Arg Lys Ile Val Cry7Aa2_MB39_NA311.1
Ala Gln Asp Ser His Leu Lys Arg Asp Val Thr Phe Ala Glu Ile Ala Ala Ala Arg Lys Ile Val g c a c a a g a c t c t c a c t t a a a a a g a g a t g t t a c a t t t g c g g a a a t t g c g g c t g c a a g a a a g a t t g t
c g t g t t c t g a g a g t g a a t t t t t c t c t a c a a t g t a a a c g c c t t t a a c g c c g a c g t t c t t t c t a a c a
3550 3560 3570 3580 3590 3600 3610
965 970 975 980
Gln Ser Ile Arg Glu Ala Tyr Met Ser Trp Leu Ser Val Val Pro Gly Val Asn His Pro Ile Cry7Aa2_MB39_NA311.1
Gln Ser Ile Arg Glu Ala Tyr Met Ser Trp Leu Ser Val Val Pro Gly Val Asn His Pro Ile c c a a t c a a t a c g c g a a g c g t a t a t g t c a t g g t t a t c t g t t g t t c c a g g t g t a a a t c a c c c t a t t t
g g t t a g t t a t g c g c t t c g c a t a t a c a g t a c c a a t a g a c a a c a a g g t c c a c a t t t a g t g g g a t a a a
g a t t g t c c a a t c a a t a c g c g a a g c g t a t a t g t c a t g g t t a t c t g t t g t t c c a g g t g t a a a t c a c c c t a t t t t 2
◄
▬ C C A A T C A A T A C G C G A A G C G T A T A T G T C A T G G T T A T C T G T T G T T C C A G G T G T A A A T C A C C C T A T T T T3620 3630 3640 3650 3660 3670 3680
985 990 995 1000 1005
Phe Thr Glu Leu Ser Gly Arg Val Gln Arg Ala Phe Gln Leu Tyr Asp Val Arg Asn Val Val Arg Cry7Aa2_MB39_NA311.1
Phe Thr Glu Leu Ser Gly Arg Val Gln Arg Ala Phe Gln Leu Tyr Asp Val Arg Asn Val Val Arg t t a c a g a g t t a a g t g g g c g a g t a c a a c g a g c a t t t c a a t t a t a t g a t g t a c g a a a t g t t g t g c g t
a a t g t c t c a a t t c a c c c g c t c a t g t t g c t c g t a a a g t t a a t a t a c t a c a t g c t t t a c a a c a c g c a
c t a t t t t t a c a g a g t t a a g t g g g c g a g t a c a a c g a g c a t t t c a a t t a t a t g a t g t a c g a a a t g t t g t g c g t a 2
◄
▬ T T A C A G A G T T A A G T G G G C G A G T A C A A C G A G C A T T T C A A T T A T A T G A T G T A C G A A A T G T T G T G C G T A3680 3690 3700 3710 3720 3730 3740
1010 1015 1020 1025
Asn Gly Arg Phe Leu Asn Gly Leu Ser Asp Trp Ile Val Thr Ser Asp Val Lys Val Gln Glu Glu Cry7Aa2_MB39_NA311.1
Asn Gly Arg Phe Leu Asn Gly Leu Ser Asp Trp Ile Val Thr Ser Asp Val Lys Val Gln Glu Glu a a t g g t c g a t t c c t c a a t g g c t t a t c c g a t t g g a t t g t a a c a t c t g a c g t a a a g g t a c a a g a a g a
t t a c c a g c t a a g g a g t t a c c g a a t a g g c t a a c c t a a c a t t g t a g a c t g c a t t t c c a t g t t c t t c t
g t g c g t a a t g g t c g a t t c c t c a a t g g c t t a t c c g a t t g g a t t g t a a c a t c t g a c g t a a a g g t a c a a g a a g a a 2
◄
▬ A A T G G T C G A T T C C T C A A T G G C T T A T C C G A T T G G A T T G T A A C A T C T G A C G T A A A G G T A C A A G A A G A A3750 3760 3770 3780 3790 3800 3810
1030 1035 1040 1045
Asn Gly Asn Asn Val Leu Val Leu Asn Asn Trp Asp Ala Gln Val Leu Gln Asn Val Lys Leu Cry7Aa2_MB39_NA311.1
a a a t g g g a a t a a c g t a t t a g t t c t t a a c a a t t g g g a t g c a c a a g t a t t a c a a a a c g t a a a a c t c t
3810 3820 3830 3840 3850 3860 3870
BssHII BsePI PauI PteI
1050 1055 1060 1065 1070
Tyr Gln Asp Arg Gly Tyr Ile Leu His Val Thr Ala Arg Lys Ile Gly Ile Gly Glu Gly Tyr Ile Cry7Aa2_MB39_NA311.1
Tyr Gln Asp Arg Gly Tyr Ile Leu His Val Thr Ala Arg Lys Ile Gly Ile Gly Glu Gly Tyr Ile a t c a a g a c c g t g g g t a t a t c t t a c a t g t a a c a g c g c g c a a g a t a g g a a t t g g g g a a g g a t a t a t a
t a g t t c t g g c a c c c a t a t a g a a t g t a c a t t g t c g c g c g t t c t a t c c t t a a c c c c t t c c t a t a t a t
a a c t c t a t c a a g a c c g t g g g t a t a t c t t a c a t g t a a c a g c g c g c a a g a t a g g a a t t g g g g a a g g a t a t a t a a 2
◄
▬ A T C A A G A C C G T G G G T A T A T C T T A C A T G T A A C A G C G C G C A A G A T A G G A A T T G G G G A A G G A T A T A T A A3880 3890 3900 3910 3920 3930 3940
1075 1080 1085 1090
Thr Ile Thr Asp Glu Glu Gly His Thr Asp Gln Leu Arg Phe Thr Ala Cys Glu Glu Ile Asp Ala Cry7Aa2_MB39_NA311.1
Thr Ile Thr Asp Glu Glu Gly His Thr Asp Gln Leu Arg Phe Thr Ala Cys Glu Glu Ile Asp Ala a c g a t t a c g g a t g a a g a a g g g c a t a c a g a t c a a t t g a g a t t t a c t g c a t g t g a a g a g a t t g a t g c
t g c t a a t g c c t a c t t c t t c c c g t a t g t c t a g t t a a c t c t a a a t g a c g t a c a c t t c t c t a a c t a c g
t a t a t a a c g a t t a c g g a t g a a g a a g g g c a t a c a g a t c a a t t g a g a t t t a c t g c a t g t g a a g a g a t t g a t g c a 2
◄
▬ A C G A T T A C G G A T G A A G A A G G G C A T A C A G A T C A A T T G A G A T T T A C T G C A T G T G A A G A G A T T G A T G C A3940 3950 3960 3970 3980 3990 4000
Mph1103I EcoT22I Zsp2I NsiI
1095 1100 1105 1110
Ser Asn Ala Phe Ile Ser Gly Tyr Ile Thr Lys Glu Leu Glu Phe Phe Pro Asp Thr Glu Lys Cry7Aa2_MB39_NA311.1
Ser Asn Ala Phe Ile Ser Gly Tyr Ile Thr Lys Glu Leu Glu Phe Phe Pro Asp Thr Glu Lys a t c t a a t g c g t t t a t a t c c g g t t a t a t t a c a a a a g a a c t g g a a t t c t t c c c a g a t a c a g a g a a a g
t a g a t t a c g c a a a t a t a g g c c a a t a t a a t g t t t t c t t g a c c t t a a g a a g g g t c t a t g t c t c t t t c
t g a t g c a t c t a a t g c g t t t a t a t c c g g t t a t a t t a c a a a a g a a c t g g a a t t c t t c c c a g a t a c a g a g a a a g t
4010 4020 4030 4040 4050 4060 4070
1115 1120 1125 1130 1135
Val His Ile Glu Ile Gly Glu Thr Glu Gly Ile Phe Leu Val Glu Ser Ile Glu Leu Phe Leu Met Cry7Aa2_MB39_NA311.1
C A T A T C T C A A T A A A A A C T A C Cry7Aa2_His_PstI_Rv Val His Ile Glu Ile Gly Glu Thr Glu Gly Ile Phe Leu Val Glu Ser Ile Glu Leu Phe Leu Met
t g c a t a t a g a a a t a g g c g a a a c a g a a g g a a t a t t c c t g g t a g a a a g t a t a g a g t t a t t t t t g a t g
a c g t a t a t c t t t a t c c g c t t t g t c t t c c t t a t a a g g a c c a t c t t t c a t a t c t c a a t a a a a a c t a c
a g a a a g t g c a t a t a g a a a t a g g c g a a a c a g a a g g a a t a t t c c t g g t a g a a a g t a t a g a g t t a t t t t t g a t g g 2
◄
▬ T G C A T A T A G A A A T A G G C G A A A C A G A A G G A A T A T T C C T G G T A G A A A G T A T A G A G T T A T T T T T G A T G G4070 4080 4090 4100 4110 4120 4130
SphI BbuI PaeI
PstI
1140 1145
Glu Glu Leu Cys His His His His His His *
Cry7Aa2_MB39_NA311.1 M13 fwd
1 5
His His His His His His * 6xHis
C T T C T C G A T A C A G T A G T A G T A G T A G T A G T A A T T G A C G T C CT T Cry7Aa2_His_PstI_Rv Glu Glu Leu Cys His His His His His His *
* Thr Met Met Met Met Met Met Leu Gln Leu Cys Ala Leu Lys Ala Ser Ala Thr Thr g a a g a g c t a t g t c a t c a t c a t c a t c a t c a t t a a c t g c a g g c a t g c a a g c t t g g c a c t g g c c g t c g
c t t c t c g a t a c a g t a g t a g t a g t a g t a g t a a t t g a c g t c c g t a c g t t c g a a c c g t g a c c g g c a g c
t t g a t g g a a g a g c t a t g t c a t c a t c a t c a t c a t c a t t a a c t g c a g g c a t g c a a g c t t g g c a c t g g c c g t c g t 2
◄
▬ G A A G A G C T A T G T C A T C A T C A T C A T C A T C A T T A A C T G C A G G C A T G C A A G C T T G G C A C T G G C C G T C G T4140 4150 4160 4170 4180 4190 4200
M13 fwd
Lys Cys Arg Arg Ser Gln Ser Phe Gly Pro Thr Val Trp Ser Leu Arg Arg Ala Ala Cys Gly t t t t a c a a c g t c g t g a c t g g g a a a a c c c t g g c g t t a c c c a a c t t a a t c g c c t t g c a g c a c a t c c
a a a a t g t t g c a g c a c t g a c c c t t t t g g g a c c g c a a t g g g t t g a a t t a g c g g a a c g t c g t g t a g g
4200 4210 4220 4230 4240 4250 4260
Gly Lys Ala Leu Gln Arg Leu Leu Ser Ser Ala Arg Val Ser Arg Gly Glu Trp Cys Asn Arg Leu c c c t t t c g c c a g c t g g c g t a a t a g c g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a
g g g a a a g c g g t c g a c c g c a t t a t c g c t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t
a c a t c c c c c t t t c g c c a g c t g g c g t a a t a g c g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g
4270 4280 4290 4300 4310 4320 4330
Arg Phe Pro Ser His Arg Arg Ile Arg Tyr Lys Arg Arg Val Cys Arg His Pro Ile Glu Cys g c c t g a a t g g c g a a t g g c g c c t g a t g c g g t a t t t t c t c c t t a c g c a t c t g t g c g g t a t t t c a c a c
c g g a c t t a c c g c t t a c c g c g g a c t a c g c c a t a a a a g a g g a a t g c g t a g a c a c g c c a t a a a g t g t g
t g c g c a g c c t g a a t g g c g a a t g g c g c c t g a t g c g g t a t t t t c t c c t t a c g c a t c t g t g c g g t a t t t c a c a c c
4330 4340 4350 4360 4370 4380 4390
Arg Met
c g c a t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g c c c c g a c a c c c
g c g t a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c g g g g c t g t g g g
t c a c a c c g c a t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g c c c c g a c a c c c g
4400 4410 4420 4430 4440 4450 4460
g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c t t g t c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g
c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g a a c a g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c
a c a c c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c t t g t c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t
4460 4470 4480 4490 4500 4510 4520
t g a c c g t c t c c g g g a g c t g c a t g t g t c a g a g g t t t t c a c c g t c a t c a c c g a a a c g c g c g a g a c g a
a c t g g c a g a g g c c c t c g a c g t a c a c a g t c t c c a a a a g t g g c a g t a g t g g c t t t g c g c g c t c t g c t
4590 4600 4610 4620 4630 4640 4650
a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a
t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t
g a c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a
4660 4670 4680 4690 4700 4710 4720
a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t c c a t c c t c c a a a g t t g g
t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a g g t a g g a g g t t t c a a c c
a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t c c a t c c t c c a a a g t t g g a
4720 4730 4740 4750 4760 4770 4780
a g a g t g a g t t t t a t g t c g c a a a t a t t a a t g t t t c t g g t g a a c c t t a t c a a a t t t t c g t t g a t t t a
t c t c a c t c a a a a t a c a g c g t t t a t a a t t a c a a a g a c c a c t t g g a a t a g t t t a a a a g c a a c t a a a t
a g t t g g a g a g t g a g t t t t a t g t c g c a a a t a t t a a t g t t t c t g g t g a a c c t t a t c a a a t t t t c g t t g a t t t a a
4790 4800 4810 4820 4830 4840 4850
a t a g a a a c a t a g c g g t a a a a t t a g c a g t a a c t t a a t a g a a c g g a a a t g a a a a a a g c c a c t c t c a t
t a t c t t t g t a t c g c c a t t t t a a t c g t c a t t g a a t t a t c t t g c c t t t a c t t t t t t c g g t g a g a g t a
g a t t t a a t a g a a a c a t a g c g g t a a a a t t a g c a g t a a c t t a a t a g a a c g g a a a t g a a a a a a g c c a c t c t c a t a
4850 4860 4870 4880 4890 4900 4910
a t g c t a t t g g c t a c c a a c c t t t a g c g a g a a t g a c t t a a t c c t g t a c a g c c a t a c a g g a c t t c g a c
t a c g a t a a c c g a t g g t t g g a a a t c g c t c t t a c t g a a t t a g g a c a t g t c g g t a t g t c c t g a a g c t g
t c t c a t a t g c t a t t g g c t a c c a a c c t t t a g c g a g a a t g a c t t a a t c c t g t a c a g c c a t a c a g g a c t t c g a c t
4920 4930 4940 4950 4960 4970 4980
Bsp68I BtuMI NruI RruI
4980 4990 5000 5010 5020 5030 5040
SpeI BcuI AhlI
c a c c t a t t g t a c c g t t a c t c t a c g a a t a t t t c a a c t a g t a a t t a c t a g c a t t g t c a t a t a c a t a a
g t g g a t a a c a t g g c a a t g a g a t g c t t a t a a a g t t g a t c a t t a a t g a t c g t a a c a g t a t a t g t a t t
t a g a t a c a c c t a t t g t a c c g t t a c t c t a c g a a t a t t t c a a c t a g t a a t t a c t a g c a t t g t c a t a t a c a t a a t
5050 5060 5070 5080 5090 5100 5110
t a a a a c g g a t a t a a a a g g g c g t t t t c t a t a c c t a g a a g t c t t g t a a a t g t a c a g g g c g t t t a g a t
a t t t t g c c t a t a t t t t c c c g c a a a a g a t a t g g a t c t t c a g a a c a t t t a c a t g t c c c g c a a a t c t a
a c a t a a t a a a a c g g a t a t a a a a g g g c g t t t t c t a t a c c t a g a a g t c t t g t a a a t g t a c a g g g c g t t t a g a t a
5110 5120 5130 5140 5150 5160 5170
a t a g a g a a c g c c c t t t t t g t g t t c c g t t c c a g t g g a a g c t a c c a c t t t a a a a a g a t g g t c t a g t g
t a t c t c t t g c g g g a a a a a c a c a a g g c a a g g t c a c c t t c g a t g g t g a a a t t t t t c t a c c a g a t c a c
t t a g a t a t a g a g a a c g c c c t t t t t g t g t t c c g t t c c a g t g g a a g c t a c c a c t t t a a a a a g a t g g t c t a g t g t
5180 5190 5200 5210 5220 5230 5240
t a g c c a a t g c a g g a g a g t a c a c t c g g a t a t c a g t t g t c g t t g c a t t c a a c t g t c t g a c g t a a g c g
a t c g g t t a c g t c c t c t c a t g t g a g c c t a t a g t c a a c a g c a a c g t a a g t t g a c a g a c t g c a t t c g c
c t a g t g t a g c c a a t g c a g g a g a g t a c a c t c g g a t a t c a g t t g t c g t t g c a t t c a a c t g t c t g a c g t a a g c g a
5240 5250 5260 5270 5280 5290 5300
a g g t a a a g g a c a c a a g c c t t g c a t a a a a c a a g c c t a c g g g a t g t a a a t c c t a a t a a t g a t g a t a a
t c c a t t t c c t g t g t t c g g a a c g t a t t t t g t t c g g a t g c c c t a c a t t t a g g a t t a t t a c t a c t a t t
5370 5380 5390 5400 5410 5420 5430
a a c a g t t a c t c g t a a g c g g c g a t c a t g a c a c t g a t t c a c g g c t a t t c t t g t a c a a g c t t t a t t a c
t t g t c a a t g a g c a t t c g c c g c t a g t a c t g t g a c t a a g t g c c g a t a a g a a c a t g t t c g a a a t a a t g
g a g t t a a a c a g t t a c t c g t a a g c g g c g a t c a t g a c a c t g a t t c a c g g c t a t t c t t g t a c a a g c t t t a t t a c a
5440 5450 5460 5470 5480 5490 5500
a a g g a t a t g c g g g t t a t a t a g c g a a t c a c c c g a a a g g g a a c g g t g t t g g g c g t g a g a a a c g c a c c
t t c c t a t a c g c c c a a t a t a t c g c t t a g t g g g c t t t c c c t t g c c a c a a c c c g c a c t c t t t g c g t g g
t a t t a c a a g g a t a t g c g g g t t a t a t a g c g a a t c a c c c g a a a g g g a a c g g t g t t g g g c g t g a g a a a c g c a c c g
5500 5510 5520 5530 5540 5550 5560
Pfl23II PspLI BsiWI
g t a c g g c g c a a t a c a a t g c c a a t a a g c t a t a t a c g g a c g g t a t a g t a g t t t t g t a a g c t a t a a c c
c a t g c c g c g t t a t g t t a c g g t t a t t c g a t a t a t g c c t g c c a t a t c a t c a a a a c a t t c g a t a t t g g
c g c a c c g t a c g g c g c a a t a c a a t g c c a a t a a g c t a t a t a c g g a c g g t a t a g t a g t t t t g t a a g c t a t a a c c g
5570 5580 5590 5600 5610 5620 5630
g t t t g t c g t c a a t g c a a c c a a t c t c a a t t c g a g a c c t c g g c a t c t a a g c c a g t a c g a a t g a g t g g
c a a a c a g c a g t t a c g t t g g t t a g a g t t a a g c t c t g g a g c c g t a g a t t c g g t c a t g c t t a c t c a c c
a t a a c c g t t t g t c g t c a a t g c a a c c a a t c t c a a t t c g a g a c c t c g g c a t c t a a g c c a g t a c g a a t g a g t g g g
5630 5640 5650 5660 5670 5680 5690
g c g t t t t a a c c t c g t a a a t t t t c a a c a g g g g t t a c t a t g c c c a a a a c t a c a t t c a g a t t t c c t a a
c g c a a a a t t g g a g c a t t t a a a a g t t g t c c c c a a t g a t a c g g g t t t t g a t g t a a g t c t a a a g g a t t
g a g t g g g c g t t t t a a c c t c g t a a a t t t t c a a c a g g g g t t a c t a t g c c c a a a a c t a c a t t c a g a t t t c c t a a c
5700 5710 5720 5730 5740 5750 5760
5760 5770 5780 5790 5800 5810 5820
t t a g c a a a a a a t g t a g a g t a c t g a a g c a a c t a c c a t t a a c t a a g a t a g t g g g g g a t t g a g g a a g a
a a t c g t t t t t t a c a t c t c a t g a c t t c g t t g a t g g t a a t t g a t t c t a t c a c c c c c t a a c t c c t t c t
c c t c g a t t a g c a a a a a a t g t a g a g t a c t g a a g c a a c t a c c a t t a a c t a a g a t a g t g g g g g a t t g a g g a a g a a
5830 5840 5850 5860 5870 5880 5890
a t c c a g a g c t g t t t a a a t c a a g t g a a a g a c a a g a t g a a a t t a a a a g a a t a g t g a a a g a t a g g g g a
t a g g t c t c g a c a a a t t t a g t t c a c t t t c t g t t c t a c t t t a a t t t t c t t a t c a c t t t c t a t c c c c t
g g a a g a a t c c a g a g c t g t t t a a a t c a a g t g a a a g a c a a g a t g a a a t t a a a a g a a t a g t g a a a g a t a g g g g a g
5890 5900 5910 5920 5930 5940 5950
g t g g t t c t c t a t g a g a a a g g a a a t g g c t a g a g a a c a a a g g c a g c g g t t t a t t g a t c t a t t g t t a g
c a c c a a g a g a t a c t c t t t c c t t t a c c g a t c t c t t g t t t c c g t c g c c a a a t a a c t a g a t a a c a a t c
a g g g g a g t g g t t c t c t a t g a g a a a g g a a a t g g c t a g a g a a c a a a g g c a g c g g t t t a t t g a t c t a t t g t t a g a
5960 5970 5980 5990 6000 6010 6020
a c t t t a t g g t a a a g a a t c c t c a t t t a t t t g t t a a t g g t a c a g a g g a t g a a a g t a a t a a t g t t g t t
t g a a a t a c c a t t t c t t a g g a g t a a a t a a a c a a t t a c c a t g t c t c c t a c t t t c a t t a t t a c a a c a a
t g t t a g a c t t t a t g g t a a a g a a t c c t c a t t t a t t t g t t a a t g g t a c a g a g g a t g a a a g t a a t a a t g t t g t t a
6020 6030 6040 6050 6060 6070 6080
a c a a a a t g t a a t a g t g a t a t t a a a g a g g t t g c g g a g t c a t a t t t a a c t c t t t t a t a g t g a g a g g g
t g t t t t a c a t t a t c a c t a t a a t t t c t c c a a c g c c t c a g t a t a a a t t g a g a a a a t a t c a c t c t c c c
g t t g t t a c a a a a t g t a a t a g t g a t a t t a a a g a g g t t g c g g a g t c a t a t t t a a c t c t t t t a t a g t g a g a g g g t
6090 6100 6110 6120 6130 6140 6150
6150 6160 6170 6180 6190 6200 6210
t t a c t a a a a g t a a g a t t a t c c a t t a g t g g a t g t t a t a a t a t t g g g t t t t t t a a c a c a a t a a t c a t
a a t g a t t t t c a t t c t a a t a g g t a a t c a c c t a c a a t a t t a t a a c c c a a a a a a t t g t g t t a t t a g t a
a c c t a c t t a c t a a a a g t a a g a t t a t c c a t t a g t g g a t g t t a t a a t a t t g g g t t t t t t a a c a c a a t a a t c a t c
6220 6230 6240 6250 6260 6270 6280
c g c c t t t c g g t g t c g t t t g a t a g a a a a g t a a c c a t t a g c g a t g a a a a a g t c a a t a t a a a a a g c c a
g c g g a a a g c c a c a g c a a a c t a t c t t t t c a t t g g t a a t c g c t a c t t t t t c a g t t a t a t t t t t c g g t
a a t c a t c g c c t t t c g g t g t c g t t t g a t a g a a a a g t a a c c a t t a g c g a t g a a a a a g t c a a t a t a a a a a g c c a t
6280 6290 6300 6310 6320 6330 6340
t c c g t a a a a a a c g g a t g g c t t a c c g t a c a t a g g a t c g t t g g t a g g g c g g c g t a t c c t a c a t c t c t
a g g c a t t t t t t g c c t a c c g a a t g g c a t g t a t c c t a g c a a c c a t c c c g c c g c a t a g g a t g t a g a g a
a a g c c a t c c g t a a a a a a c g g a t g g c t t a c c g t a c a t a g g a t c g t t g g t a g g g c g g c g t a t c c t a c a t c t c t g
6350 6360 6370 6380 6390 6400 6410
g g t a a c t t a c c t a g c c a a t c a a a t g c t t g a g a a c g g c g g t t a g a t a a g c g c g t g g g g a a c c t t t c
c c a t t g a a t g g a t c g g t t a g t t t a c g a a c t c t t g c c g c c a a t c t a t t c g c g c a c c c c t t g g a a a g
a t c t c t g g t a a c t t a c c t a g c c a a t c a a a t g c t t g a g a a c g g c g g t t a g a t a a g c g c g t g g g g a a c c t t t c c
6410 6420 6430 6440 6450 6460 6470
c c a c c t c a a a g a t c c t a t a t c a t t a t t a t g t t a c t t t c t a c a g g t a g t a t a c c a t g t t c t t a t a t
g g t g g a g t t t c t a g g a t a t a g t a a t a a t a c a a t g a a a g a t g t c c a t c a t a t g g t a c a a g a a t a t a
c c t t t c c c a c c t c a a a g a t c c t a t a t c a t t a t t a t g t t a c t t t c t a c a g g t a g t a t a c c a t g t t c t t a t a t t
6480 6490 6500 6510 6520 6530 6540
* Ser Leu Leu Asp Lys Tyr Ala Ile Leu Arg Gly Ser Asn Leu Arg t t t a g t a a a c t c c c c g t t a g c t t a a c a g g t c t t t g t a a g c a a t t a a a c g t c c a c t a t t c a a t c g t
6540 6550 6560 6570 6580 6590 6600
Arg Gln Ile Lys Ala Pro Gly Asn Lys Leu Asp Phe Met Thr Ser Leu Phe Leu Tyr Gly Ser Pro c t t t g g a t t t t c g c a g g a c c g t t t t t t a g a t c g a a c a t a g t t g a t a a g a a c a a a t a a c c g c t t g g
g a a a c c t a a a a g c g t c c t g g c a a a a a a t c t a g c t t g t a t c a a c t a t t c t t g t t t a t t g g c g a a c c
a a t c g t c t t t g g a t t t t c g c a g g a c c g t t t t t t a g a t c g a a c a t a g t t g a t a a g a a c a a a t a a c c g c t t g g g
6610 6620 6630 6640 6650 6660 6670
Asp Leu Lys Ile Ala Ile Leu Ile His Asp Asn Leu Ile Lys Val Leu Glu Val Ser Asn Pro Glu g t c c a a c t t t a t a g c a a t t a g t a t a t g g t c a t t t a a a a t c t t t a c c a a t t c a a c g c t a t t a g g t t
c a g g t t g a a a t a t c g t t a a t c a t a t a c c a g t a a a t t t t a g a a a t g g t t a a g t t g c g a t a a t c c a a
g c t t g g g t c c a a c t t t a t a g c a a t t a g t a t a t g g t c a t t t a a a a t c t t t a c c a a t t c a a c g c t a t t a g g t t c
6670 6680 6690 6700 6710 6720 6730
Tth111I AspI PflFI PsyI
Lys Pro Asn Gln Gly Val Tyr Asp Pro His Glu Val Ile Asp Lys Ile His Ser Ser Tyr Lys c t t t a g g a t t t t g c c c g a c a t a g t c g g g g t g t t c a a c g a t a t c t t t t a t g t g c g a t g a a t a t t t t
g a a a t c c t a a a a c g g g c t g t a t c a g c c c c a c a a g t t g c t a t a g a a a a t a c a c g c t a c t t a t a a a a
t a g g t t c t t t a g g a t t t t g c c c g a c a t a g t c g g g g t g t t c a a c g a t a t c t t t t a t g t g c g a t g a a t a t t t t t
6740 6750 6760 6770 6780 6790 6800
Met Leu Phe Leu Tyr Val Leu Tyr Lys Ser Gly Lys His Phe Tyr Ile Val Glu Tyr Ile Gly Pro His Gln Lys Lys Val His Lys Ile Phe Gly Pro Phe Met
t c a t a a a t a c c a g g a t g t t g t t t c t t t a c g t g c t t t a t a a a t c c g g g a a a c a t t t t t a c a t c g t t
a g t a t t t a t g g t c c t a c a a c a a a g a a a t g c a c g a a a t a t t t a g g c c c t t t g t a a a a a t g t a g c a a
t a t t t t t c a t a a a t a c c a g g a t g t t g t t t c t t t a c g t g c t t t a t a a a t c c g g g a a a c a t t t t t a c a t c g t t a
6870 6880 6890 6900 6910 6920 6930
Ile Tyr Asn Leu Phe Phe Ile Ser Arg Gln Ile Ser Pro Ser Ser Arg Leu Phe Ile Ser Leu t a t t t a c a a t c t t t t t t t t a t t a g c c g t c a a a t t t c t c c c t c a t c t c g t c t c t t t a t a t c t t t a t
a t a a a t g t t a g a a a a a a a a t a a t c g g c a g t t t a a a g a g g g a g t a g a g c a g a g a a a t a t a g a a a t a
t t g g t t t a t t t a c a a t c t t t t t t t t a t t a g c c g t c a a a t t t c t c c c t c a t c t c g t c t c t t t a t a t c t t t a t t
6930 6940 6950 6960 6970 6980 6990
Phe Tyr His Lys Gly Val Phe Glu Pro Ser Arg Gly Thr Gly Leu *
t t t a t c a t a a a g g a g t a t t t g a a c c g t c g c g c g g g a c a g g t t t a t g a t a g g g a t a t t t t a t t g a a
a a a t a g t a t t t c c t c a t a a a c t t g g c a g c g c g c c c t g t c c a a a t a c t a t c c c t a t a a a a t a a c t t
c t t t a t t t t a t c a t a a a g g a g t a t t t g a a c c g t c g c g c g g g a c a g g t t t a t g a t a g g g a t a t t t t a t t g a a t
7000 7010 7020 7030 7040 7050 7060
t a a t t g a t g g t a t a a g g g a c t t t c a t g c t t g g a a a g t g g g g a t t a t g a a t t a g a t g c t t g t c c a c
a t t a a c t a c c a t a t t c c c t g a a a g t a c g a a c c t t t c a c c c c t a a t a c t t a a t c t a c g a a c a g g t g
a t t g a a t a a t t g a t g g t a t a a g g g a c t t t c a t g c t t g g a a a g t g g g g a t t a t g a a t t a g a t g c t t g t c c a c a
7060 7070 7080 7090 7100 7110 7120
a a t a t g t t c c a a t g t a a t t a a a a t t t a t g t t c c c a c c t t g a c c a a a c a t c a c g t c c a t a c t t a a a
t t a t a c a a g g t t a c a t t a a t t t t a a a t a c a a g g g t g g a a c t g g t t t g t a g t g c a g g t a t g a a t t t
g t c c a c a a t a t g t t c c a a t g t a a t t a a a a t t t a t g t t c c c a c c t t g a c c a a a c a t c a c g t c c a t a c t t a a a t
7130 7140 7150 7160 7170 7180 7190
Met Asp Asn Ser Ile t c g t c c c t c c t t t a a t a g g t a a a a t a t t a a t t t a c c t t a a t a a a a a a a t a a t g g a t a a t a g t a t t
a g c a g g g a g g a a a t t a t c c a t t t t a t a a t t a a a t g g a a t t a t t t t t t t a t t a c c t a t t a t c a t a a
c t t a a a t c g t c c c t c c t t t a a t a g g t a a a a t a t t a a t t t a c c t t a a t a a a a a a a t a a t g g a t a a t a g t a t t c
7190 7200 7210 7220 7230 7240 7250
7260 7270 7280 7290 7300 7310 7320
Thr Asp Val Val Lys Asp Leu Gln Asp Lys Val Ile Ser Leu Gln Asp His Glu Val Ala Phe t a c t g a t g t c g t a a a g g a t t t g c a a g a t a a a g t t a t a t c a t t g c a g g a t c a t g a g g t a g c g t t t t
a t g a c t a c a g c a t t t c c t a a a c g t t c t a t t t c a a t a t a g t a a c g t c c t a g t a c t c c a t c g c a a a a
g c c a t c t a c t g a t g t c g t a a a g g a t t t g c a a g a t a a a g t t a t a t c a t t g c a g g a t c a t g a g g t a g c g t t t t t
7320 7330 7340 7350 7360 7370 7380
Leu Asn Thr Thr Ile Ser Asn Met Leu Thr Ala Val Gly Ile Gly Val Ala Ile Ile Thr Ala Val t g a a c a c c a c g a t a t c t a a t a t g t t a a c a g c a g t a g g t a t t g g a g t g g c a a t t a t a a c g g c g g t t
a c t t g t g g t g c t a t a g a t t a t a c a a t t g t c g t c a t c c a t a a c c t c a c c g t t a a t a t t g c c g c c a a
c g t t t t t g a a c a c c a c g a t a t c t a a t a t g t t a a c a g c a g t a g g t a t t g g a g t g g c a a t t a t a a c g g c g g t t t
7390 7400 7410 7420 7430 7440 7450
Phe Thr Ala Ala Phe Ala Tyr Val Thr Tyr Ser Asn Lys Arg Ala Lys Lys Asn Met Asp Glu Ala t t t a c a g c a g c g t t t g c t t a t g t t a c a t a t t c t a a t a a g c g t g c t a a a a a g a a t a t g g a c g a g g c
a a a t g t c g t c g c a a a c g a a t a c a a t g t a t a a g a t t a t t c g c a c g a t t t t t c t t a t a c c t g c t c c g
g c g g t t t t t a c a g c a g c g t t t g c t t a t g t t a c a t a t t c t a a t a a g c g t g c t a a a a a g a a t a t g g a c g a g g c t
7450 7460 7470 7480 7490 7500 7510
Ser Arg Lys Leu Glu Glu Ala Glu Ser Lys Val Ser Val Leu Glu Glu Lys Ser Ala Gln Leu t a g t a g a a a a t t a g a a g a a g c a g a a a g t a a a g t t t c t g t g c t a g a g g a g a a a a g c g c t c a a t t g g
a t c a t c t t t t a a t c t t c t t c g t c t t t c a t t t c a a a g a c a c g a t c t c c t c t t t t c g c g a g t t a a c c
c g a g g c t a g t a g a a a a t t a g a a g a a g c a g a a a g t a a a g t t t c t g t g c t a g a g g a g a a a a g c g c t c a a t t g g a
7520 7530 7540 7550 7560 7570 7580
BamHI
a g a g g a a g a t t c t t g a a g c t g a a c a a c t c t t a g c t g a t g c c a a t t c t a t t t c t a a t g t g g g a t c c
7580 7590 7600 7610 7620 7630 7640
BsgI
Pro *
c c t t a g a a g c a a a c t t a a g a g t g t g t t g a t a g t g c a g t a t c t t a a a a t t t t g t g t a t a a t a g g a a
g g a a t c t t c g t t t g a a t t c t c a c a c a a c t a t c a c g t c a t a g a a t t t t a a a a c a c a t a t t a t c c t t
g g a t c c c c t t a g a a g c a a a c t t a a g a g t g t g t t g a t a g t g c a g t a t c t t a a a a t t t t g t g t a t a a t a g g a a t
7650 7660 7670 7680 7690 7700 7710
t t g a a g t t a a a t t a g a t g c t a a a a a t t t g t a a t t a a g a a g g a g g g a t t c g t c a t g t t g g t a t t c c
a a c t t c a a t t t a a t c t a c g a t t t t t a a a c a t t a a t t c t t c c t c c c t a a g c a g t a c a a c c a t a a g g
t a g g a a t t g a a g t t a a a t t a g a t g c t a a a a a t t t g t a a t t a a g a a g g a g g g a t t c g t c a t g t t g g t a t t c c a
7710 7720 7730 7740 7750 7760 7770
a a a t g c g t a a t g t a g a t a a a a c a t c t a c t g t t t t g a a a c a g a c t a a a a a c a g t g a t t a c g c a g a t
t t t a c g c a t t a c a t c t a t t t t g t a g a t g a c a a a a c t t t g t c t g a t t t t t g t c a c t a a t g c g t c t a
t a t t c c a a a t g c g t a a t g t a g a t a a a a c a t c t a c t g t t t t g a a a c a g a c t a a a a a c a g t g a t t a c g c a g a t a
7780 7790 7800 7810 7820 7830 7840
a a a t a a a t a c g t t a g a t t a a t t c c t a c c a g t g a c t a a t c t t a t g a c t t t t t a a a c a g a t a a c t a a
t t t a t t t a t g c a a t c t a a t t a a g g a t g g t c a c t g a t t a g a a t a c t g a a a a a t t t g t c t a t t g a t t
g c a g a t a a a t a a a t a c g t t a g a t t a a t t c c t a c c a g t g a c t a a t c t t a t g a c t t t t t a a a c a g a t a a c t a a a
7840 7850 7860 7870 7880 7890 7900
a a t t a c a a a c a a a t c g t t t a a c t t c t g t a t t t a t t t a c a g a t g t a a t c a c t t c a g g a g t a a t t a c
t t a a t g t t t g t t t a g c a a a t t g a a g a c a t a a a t a a a t g t c t a c a t t a g t g a a g t c c t c a t t a a t g
a a c t a a a a t t a c a a a c a a a t c g t t t a a c t t c t g t a t t t a t t t a c a g a t g t a a t c a c t t c a g g a g t a a t t a c a
7910 7920 7930 7940 7950 7960 7970
7970 7980 7990 8000 8010 8020 8030
Lys Gln Leu Asn Leu Lys Glu Thr Asp Thr Val Tyr Glu Ile Gly Thr Gly Lys Gly His Leu a a a a c a a t t g a a t t t a a a a g a a a c c g a t a c c g t t t a c g a a a t t g g a a c a g g t a a a g g g c a t t t a a
t t t t g t t a a c t t a a a t t t t c t t t g g c t a t g g c a a a t g c t t t a a c c t t g t c c a t t t c c c g t a a a t t
a a t a a t a a a a c a a t t g a a t t t a a a a g a a a c c g a t a c c g t t t a c g a a a t t g g a a c a g g t a a a g g g c a t t t a a c
8040 8050 8060 8070 8080 8090 8100
Thr Thr Lys Leu Ala Lys Ile Ser Lys Gln Val Thr Ser Ile Glu Leu Asp Ser His Leu Phe Asn c g a c g a a a c t g g c t a a a a t a a g t a a a c a g g t a a c g t c t a t t g a a t t a g a c a g t c a t c t a t t c a a c
g c t g c t t t g a c c g a t t t t a t t c a t t t g t c c a t t g c a g a t a a c t t a a t c t g t c a g t a g a t a a g t t g
a t t t a a c g a c g a a a c t g g c t a a a a t a a g t a a a c a g g t a a c g t c t a t t g a a t t a g a c a g t c a t c t a t t c a a c t
8100 8110 8120 8130 8140 8150 8160
Leu Ser Ser Glu Lys Leu Lys Leu Asn Ile Arg Val Thr Leu Ile His Gln Asp Ile Leu Gln Phe t t a t c g t c a g a a a a a t t a a a a c t g a a c a t t c g t g t c a c t t t a a t t c a c c a a g a t a t t c t a c a g t t
a a t a g c a g t c t t t t t a a t t t t g a c t t g t a a g c a c a g t g a a a t t a a g t g g t t c t a t a a g a t g t c a a
t t c a a c t t a t c g t c a g a a a a a t t a a a a c t g a a c a t t c g t g t c a c t t t a a t t c a c c a a g a t a t t c t a c a g t t t
8170 8180 8190 8200 8210 8220 8230
Gln Phe Pro Asn Lys Gln Arg Tyr Lys Ile Val Gly Ser Ile Pro Tyr His Leu Ser Thr Gln t c a a t t c c c t a a c a a a c a g a g g t a t a a a a t t g t t g g g a g t a t t c c t t a c c a t t t a a g c a c a c a a a
a g t t a a g g g a t t g t t t g t c t c c a t a t t t t a a c a a c c c t c a t a a g g a a t g g t a a a t t c g t g t g t t t
a c a g t t t c a a t t c c c t a a c a a a c a g a g g t a t a a a a t t g t t g g g a g t a t t c c t t a c c a t t t a a g c a c a c a a a t
8230 8240 8250 8260 8270 8280 8290
Ile Ile Lys Lys Val Val Phe Glu Ser His Ala Ser Asp Ile Tyr Leu Ile Val Glu Glu Gly Phe t t a t t a a a a a a g t g g t t t t t g a a a g c c a t g c g t c t g a c a t c t a t c t g a t t g t t g a a g a a g g a t t c
