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Expression of sex specific molecular markers in clones of bipartite allophenic nemertines produced by somatic embryogenesis from Lineus sanguineus male/female chimera fragments

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Academic year: 2020

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Figure

Fig. 1. LineussanguineusallogenicchimerasFU and UFo Two symmet-ric alfogencicchimeras.FU and UF, were constructedbygraftlngthe lateralhalves from a phenotypicallydark-brownmale (F, France) onto the anatomi-cally complementaryhalvesfrom a phenotypicallylight-brownfemale(U,Uruguay).Thus,the 2 halvesof eachheterosexualchimeraformedby'graftingthe righthalf of onewormontothe lefthalf of a wormof theoppositesex had differentcolors.The FU chimerais mirror image of the UFchimera.Dark circlesrepresenttestis,and fight circlesovaries.(Modifiedfrom Sivaradjamand Blerne,1989).
Fig. 3. Electrophoretic-and matureprotein present.patternof proteinsextractedfromimmatureIi)1m) Lineus sanguineusfemales(U) and malesIF)
Fig.5.1/9 per lane)ElectrophoregramsofproteinsextractedfromabipartiteallophenicLineussanguineusin the 19th year of cloning.Proteins(40 were separatedby 50S-PAGEand stainedwith Coomassieblue

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