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Isolation and characterization of human fibroblast tenascin An extracellular matrix glycoprotein of interest for developmental studies

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Figure

Fig. 2. Purificationstrategyschemefor humanfibroblastTN.
Fig. 3. Distributionof proteinandantigenicprofileof TN andFN on aSepharoseCl4B columnchromatography.One hundredmg of 2M ureaextractappliedon a SepharoseCL48columnandeluted.(a) 280nmabsorbance Ibl Distributionof Immunogenicityof TN (T-))and of FN(e_.).
Fig. 5. 5DS-PAGEanalysisand Immunoblot1ingof FractionII. (a) 50S-PAGEof the DEAE-5PW ion-exchangecolumnfractions (numbers40 to 46shownIn Fig
Fig. 6. DEAE- 5PW ion-exchangechromatographyconditionedof FractionA frommediumaftergelatinSepharose48 chromatography.Elution condition was same asIn Fig
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