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Third-stage Gnathostoma spinigerum larva excretory secretory antigens modulate function of Fc gamma receptor I-mediated monocytes in peripheral blood mononuclear cell culture

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Fig. 1 Determination of the appropriate G. spinigerum ESA concentration for PBMC culture; 2 million PBMC (CD27−) were cultured in RPMI 1640supplemented with 10 % of inactivated FBS alone or plus IL-2 (10 ng/ml) or ESA (0.1, 0.5, and 1 μg/ml)
Fig. 3 G. spinigerum ESA modulated the expression of FcγRI on monocytes. Approximately 2 × 106 PBMC (CD27−) were cultured in medium aloneor in medium plus IL-2 (10 ng/ml), TGF-β1 (100 pg/ml), or ESA (0.1 μg/ml) for 12 h
Fig. 4 G. spinigerum ESA modulated phagocytic capacity of monocytes. Monocyte-enriched adherent cells from PBMC cultured in medium alone,plus IL-2 (10 ng/ml) or ES (0.1 μg/ml) for 12 h were incubated with zymosan (ZM)

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