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Construct design, production, and characterization of Plasmodium falciparum 48/45 R0.6C subunit protein produced in Lactococcus lactis as candidate vaccine

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Academic year: 2020

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Figure

Fig. 1 Constructs for expression of R0.6C in L. lactis. a Schematic representation of Pfs48/45 and the R0.6C constructs: 1 C-terminal 6xHis-tag, 2 codon optimized genes (hiligheted with color shades), 3 no tag, 4 N-terminal Spytag, 5 N-termianl SpyCatcher,
Fig. 2 Time course analysis of the expression of R0.6C in aexpression of R0.6C. lyzed
Fig. 3 Expression and purification of R0.6C. a Schematic representation of expression and purification R0.6C fusion protein
Fig. 5 Size exclusion and reversed-phase HPLC analysis of purified R0.6C. a Size exclusion chromatography was performed under native conditions in a phosphate buffer pH 6.7, to determine the amount of monomer in the sample
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