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Improved methodology for assaying brassinosteroids in plant tissues using magnetic hydrophilic material for both extraction and derivatization

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Figure 1 Effect of different magnetic sorbents on the BR recoveries in acetonitrile solution sample (n = 2) (A) and plant extract (n = 3) (B).BRs were spiked in the sampling solution at 1 ng/mL each.
Figure 3 Effect of the DMAPBA amount on the derivatization efficiencies of BRs in acetonitrile (n = 3) (A) and in plant extract (n = 3) (B).BRs were spiked in the sampling solution at 1 ng/mL each.
Figure 6 MRM chromatograms of the BRs in plant tissue (100 mgFW) treated with the MSPE-ISD method
Table 2 Accuracy and precision (intra- and inter-day) for the determination of BRs in O
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