1 Reversed-Phase C18 Columns (ODS)

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YMC GENERAL CATALOG

1

Reversed-Phase

C18 Columns (ODS)

Types and characteristics of C18

packing materials

Guides for selecting C18 packing materials

YMC-Triart C18

YMC-UltraHT series

YMC-UltraHT

Pro

C18

YMC-UltraHT Hydrosphere C18

Pro series

Features of

Pro

series ODS columns

YMC-Pack

Pro

C18

Hydrosphere C18

YMC-Pack

Pro

C18 RS

YMC-Pack ODS series

YMC-Pack ODS-A

YMC-Pack ODS-AM

YMC-Pack ODS-AQ

YMC-Pack ODS-AL

J

,

sphere ODS series

ODS-H80

、

M80

、

L80

YMC-Pack PolymerC18

14

15

16

∼

23

24,25

26

27

28

∼

31

32

∼

35

36

∼

39

40

∼

43

44,45

46,47

48,49

50,51

52,53

54,55

13

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Reversed-Phase C18 Columns

（

ODS

）

Types of C18 packing materials

Base Products

Hybrid silica

YMC-Triart C18

Silica

YMC-UltraHT series YMC-Pack Pro C18 Hydrosphere C18 YMC-Pack Pro C18 RS YMC-Pack ODS series J’sphere ODS series

Polymer

YMC-Pack PolymerC18

Types and characteristics of C18 (ODS) packing materials

Product name Pore size

（Å） Particle size （µm） ODS Usable

pH range Characteristics Pages

C% _treatmentSilanol YMC-Triart C18

120 2

20 Yes 1.0 〜12.0

・ Versatile hybrid silica based ODS column

・Great chemical durability ・ Excellent peak shape in every conditions

16 〜 23 3 5 UltraHT series Pro C18 120 2 16 Yes 2.0 〜 8.0

・ 2 µm-ODS column for Ultra-fast LC method

・ Superior separation of basic compounds

26

Hydrosphere C18 120 2 12

・ Superior separation of hydrophilic compounds ・ Reduced analysis time while

maintaining resolution

27

Pro series

Pro C18 120 3 16 Yes

2.0 〜 8.0

・ Processed with YMC Co., Ltd.’s advanced endcapping technology

・ Superior separation of basic compounds 32 〜 35 5 Hydrosphere C18 120 3 12 Yes ・ Superior separation of hydrophilic compounds

・ Can be used with 100% water mobile phase

36 〜 39 5

Pro C18 RS 80 3 22 Yes 1.0 〜10.0 ・Highly durable ODS・ Superior separation of basic compounds

and hydrophobic compounds 40 〜 43 5

YMC-Pack ODS series

ODS-A 120 3 17 Yes 2.0 〜 7.5

・ Currently in use worldwide 44,45 5

10

200 5 12 ・ODS with wide pore size

・ For separation of peptides

and proteins 44,45

10 300 ₁₀5 7

ODS-AM 120 3₅ 17 Yes ・ Outstanding lot-to-lot reproducibility 46,47

ODS-AQ

120 3

14

Yes ・ Superior separation of _{hydrophilic compounds} 48,49 5

10 200 ₁₀5 10

ODS-AL 120 5 17 No ・ For separation utilizing residual silanol 50,51 J'sphere ODS series ODS-H80 80 4 22 Yes 1.0 〜 9.0 ・High carbon ODS

52,53

ODS-M80 80 4 14 Yes _{2.0 〜 7.5} ・Medium carbon ODS

ODS-L80 80 4 9 Yes ・Low carbon ODS

YMC-Pack PolymerC18 _— 6 _— — _{2.0 〜13.0 ・Polymer-based ODS} _54,55

10 —

Types and characteristics of C18 packing materials

Octadecyl-silica (ODS) is the industry standard packing material for HPLC applications. YMC Co., Ltd. offers packing materials with an impressive assortment of functional groups for liquid chromatography and a selection of ODS columns that is far ahead of all others in variety. We also offer hybrid-silica based C18 packing materials that are widely applicable to a variety of fields, from microanalysis to large-scale isolation.

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Reversed-Phase C18 Columns （ ODS ）

Low molecular

weight compounds

High molecular

weight compounds

Alkaline mobile phase

General organic compounds

Basic compounds

Coordination compounds

Sugars and glycosides

YMC-Triart C18 YMC-Pack Pro C18 YMC-Pack ODS-A J’sphere ODS-M80 YMC-Pack Pro C18 RS J’sphere ODS-H80 YMC-Pack ODS-A (Wide-Pore) J’sphere ODS-L80 Hydrosphere C18 YMC-Pack Pro C18 RS YMC-Pack ODS-AL J’sphere ODS-H80 J’sphere ODS-L80 YMC-Pack PolymerC18 YMC-Triart C18 YMC-Pack Pro C18 Hydrosphere C18 YMC-Pack Pro C18 RS YMC-Pack ODS-AM YMC-Pack PolymerC18 YMC-Triart C18 YMC-Pack Pro C18 Hydrosphere C18 YMC-Pack Pro C18 RS YMC-Pack ODS-AM YMC-Pack PolymerC18 Hydrosphere C18 YMC-Pack ODS-AQ J’sphere ODS-L80

YMC-Pack ODS-A (Wide-Pore)

YMC-Triart C18 YMC-Pack Pro C18 RS J’sphere ODS-H80 YMC-Pack PolymerC18

Chemical structures diﬀer

slightly among solutes

Hydrophobicity diﬀers

greatly among solutes

Diﬃcult to separate

using standard ODS

High molecular weight peptides

and oligonucleic acids

Guides for selecting C18 packing materials

YMC Co., Ltd. offers twelve types of ODS columns, each with unique separation characteristics. The proper selection of packing material is therefore a key factor in the establishment of efficient separation conditions. Selection of packing materials without sufficient consideration of the separation characteristics may result in an expense of much time and effort in separating a target material.

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（

ODS

）

Versatile Hybrid Silica Based HPLC Column

YMC-Triart C18

YMC-Triart C18 is a hybrid silica based ODS column emphasizing durability, low operating pressure, and excellent performance. YMC-Triart C18 has been developed and is manufactured using state-of-the-art technologies that define materials, granulation and surface modification.

YMC-Triart C18　－Integration of triple artistic technologies－

＜1＞Versatile hybrid material

YMC-Triart C18 is a multi-layer hybrid particle consisting of two types of layers: a silica based layer (inorganic) and a hybrid polymer based layer (organic). This layered structure contributes to Triart's combined physical durability and chromatographic performance.

Image structure for hybrid-silica gel

inorganic-silane layer organic-silane layer

Particle

synthesis by

Microreaction

Particle

synthesis by

Microreaction

Versatile

hybrid silica

material

Versatile

hybrid silica

material

Multi-stage

endcapping

Multi-stage

endcapping

Rugged, long lasting, hybrid particle technology

Integration of triple artistic technologies

Specifications

Base ：organic/inorganic hybrid silica Stationary ：C18 (as USP-L1)

Particle ：2 µm, 3 µm, 5 µm Pore ：120 Å

Carbon % ：20%

Bonding ：polymeric type Endcapping ：multi-stage endcapping pH range ：pH 1 〜 12

Features

● Hybrid silica base ▼ Usable over a wide pH range makes the column excellent for method development.

● Great chemical durability ▼ Long column lifetime over wide pH range. 　Low cost per analysis.

● Lower back pressure ▼ Ability to use on both older and newer instrumentation. ● Excellent resolution ▼ Rugged, first choice option for analysis of acids, bases and

neutral compounds.

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YMC-Triart C18 has a very narrow pore size distribution that enables narrow and reproducible peak shapes for analytes.

Narrow pore distribution

Silica particle for conventional product 350 300 250 200 Pore size (Å) 150 100 50 0 4500 4000 3500 3000 2500 2000 1500 1000 500 0 d Vp/d logD

Hybrid particle for competitor's product Hybrid particle for Triart

＜2＞New granulation process by microreactor technology

Homogeneous and uniform particle

Use of microreactor technology produces homogeneous and uniform particles that results in low operating pressure and very reproducible surface modification making for the ultimate in column-to-column and lot-to-lot reproducibility.

Triart C18

YMC-Triart C18　－Integration of triple artistic technologies－

Column ：5 µm, 150×3.0 mmI.D.

Eluent ：20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (65/35)

Flow rate ：0.425 mL/min Temperature ：40 ℃ Detection ：UV at 235 nm

Sample ： 1. Chlorpheniramine　　2. Dextromethorphan 3. Propyl p-hydroxybenzoate

Endcapping methods employed for production of conventional products typically employ a single endcapping compound in one chemical step to "neutralize" highly reactive silanols. Unfortunately, highly reactive silanols are easily hydrolyzed and contribute to poor durability. Additionally, low reactive silanols endcap stubbornly, producing a surface modification that is often non-reproducible, and leading to peak tailing. YMC-Triart C18 solves this problem by using an innovative, newly developed "measured multi-stage/multi-compound surface modification" technology which results in the thorough endcapping of all silanols that are accessible to analytes.

＜3＞Surface modification technology

Multi-stage endcapping

Reaction image for "multi-stage endcapping"

3

STEP

Silanol group

Endcapping (EC) group

1

STEP STEP

2

Before EC

Evaluation of silanol activity

1 2 3 Triart C18 Endcapping by traditional method

Peak tailing for basic compounds

min 15.0 10.0 5.0 0.0 mAU 60 1 2 3 Q090928E Q090928C 40 20 0 min 15.0 10.0 5.0 0.0 mAU 60 40 20 0

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1

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ODS

）

YMC-Triart C18

Quality Control　－Excellent reproducibility－

1. Chlorpheniramine 3. Propyl p-hydroxybenzoate 2. Dextromethorphan

Basic compounds

Lot. E Lot. D Lot. C Lot. B Lot. A min 17.5 15.0 12.5 10.0 7.5 5.0 2.5 0.0 1 3 2 J091201Y

YMC-Triart C18 exhibits excellent lot-to-lot reproducibility for all types of compounds including difficult to chromatograph molecules that exhibit basic and coordination compound behavior.

Packing Material

Rigid control of theoretical plate number (N) and tailing factor (Tf) are exhibited for each YMC-Triart C18 packed column. Packed Column Lot. E Lot. D Lot. C Lot. B Lot. A min 14.0 12.0 10.0 8.0 6.0 4.0 2.0 0.0 1 2 J091201X 1. Hinokitiol 2. Methyl benzoate 20000 15000 10000 5000 0 2.5 2.0 1.5 1.0 0.5 10 20 30 40 50

Theoretical plate number

Tailing factor

Column number

Theor

etical plate number Tailing factor

Column ：5 µm, 150×3.0 mmI.D. Eluent ：acetonitrile/0.1% H3PO4 (40/60)

Column ：5 µm, 150×4.6 mmI.D. Eluent ：acetonitrile/water (60/40) Flow rate ：1.0 mL/min

Temperature ：ambient Sample ：butyl benzoate

Low back-pressure　－Free from pressure problem－

Column pressure and solvents

YMC-Triart C18 is designed for use in all conditions. Even with higher viscosity methanol, YMC-Triart C18 can operate with lower pressure (typically 30% less than conventional columns).

Eluent ：acetonitrile/water or methanol/water Flow rate ：1.0 mL/min

Temperature ：25 ℃

0 10 20 30 40 50 60 70 80 90 100

Organic solvent rate (%)

Column pr essu re (MPa ) 0 2 4 6 8 10 12 14 16 18 20 Triart C18, ACN/W Pro C18, ACN/W Triart C18, MeOH/W Pro C18, MeOH/W

Standard ODS　－Best balance hydrophobicity－

Comparison of separation selectivity

Conventional hybrid silica based ODS columns tend to be less hydrophobic than silica based columns. YMC-Triart C18 has a favorable carbon balance and may be used as a "versatile first-choice" method development column.

XTerra MS C18 (S090805A) Standard ODS Gemini NX-C18 2.00 4.00 6.00 8.00 10.00 12.00 14.00 Triart C18 Hydrogen-bonding capacity α (caffeine/benzene) Hydrophobicity k’ (amylbenzene)

The most versatile columns as first-choice.

Inertsil ODS-4

Eluent : MeOH/W (80/20) [amylbenzene], MeOH/W (30/70) [caffeine, benzene]

Pro C18 RS Develosil ODS-SR Develosil C30-UG Atlantis T3 Hydrosphere C18 CAPCELL PAK C18 AQ Pro C18 Gemini C18 Hypersil GOLD XBridge C18 Eternity-C18 Cadenza CD-C18 L-column2 ODS TSKgel ODS-100S L-column ODS

Mightysil RP-18SunFireC18 Inertsil ODS-3

CAPCELL PAK C18 MGII CAPCELL PAK C18 MGIII

Unison UK-C18 0.040 0.060 0.080 0.100 0.120 0.140 0.160 0.180

Coordination compounds

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Great durability　－Wide usable pH range and long lifetime－

Phosphate buffer (pH 11.5, 40 ℃)

0 10 20 30 40 50

Time (hr)

Rate of theoretical plate number

YMC-Triart C18

Silica based C18 Hybrid-silica based C18

0% 20% 40% 60% 80% 100% 120% Durability in high pH

Durability in high temperature

0 50 100 150 200 250 300

Time (hr)

0% 20% 40% 60% 80% 100% 120% YMC-Triart C18 Silica based C18 Column ：5 µm, 150×4.6 mmI.D.

Eluent ：50 mM K2HPO4-K3PO4 (pH 11.5)/methanol (90/10)

Flow rate ：1.0 mL/min Temperature ：40 ℃ Sample ：benzyl alcohol

Eluent ：50 mM triethylamine (pH 11.5)/methanol (90/10) Flow rate ：1.0 mL/min

Temperature ：40 ℃ Sample ：benzyl alcohol

YMC-Triart C18 shows great chemical stability provided by newly developed hybrid silica. Even in high pH or high temperature conditions, the lifetime of YMC-Triart C18 is more than 10 times greater than conventional ODS columns.

YMC-Triart C18

Time (hr)

Silica based C18 Silica based C18 alkaline-resistant type 0% 20% 40% 60% 80% 100% 120% 0 100 200 300 400 500 600 700 0 20 40 60 80 100 120 Time (hr) Rate of r etention time 0% 20% 40% 60% 80% 100% 120% YMC-Triart C18 Silica based C18

The columns are kept in acetonitrile/water/TFA (10/90/1), 70 °C, and tested for performance every 20 hours.

Hybrid-silica based C18

Flow rate ：0.2 mL/min Temperature ：70 ℃ Sample ：phenol

Test conditions Column ：5 µm, 50×2.0 mmI.D. Eluent ：acetonitrile/water (60/40) Flow rate ：0.2 mL/min

Temperature ：37 ℃ Sample ：butyl benzoate

Reduced column bleed　－Operate at high sensitivity－

Bleed test by LC/MS

No column Triart C18 Scan ES+ TIC 0.0E+00 4.0E+08 8.0E+08 1.2E+09 min 12 10 8 6 4 2 0 − −

Bleeding of stationary phase can often contribute to high background signals when employing MS and other hyphenated detection techniques. In the example cited above, use of a 50×2.0 mmI.D. YMC-Triart C18 column shows no contribution to the signal observed by the MS positive total ion current (TIC) trace. Such performance allows for low limits of detection (high S/N ratio) for MS and other detection techniques. The inherent hybrid particle strength is also superior to conventional silica materials for detection techniques that are sensitive to particle degradation.

Column ：5 µm, 50×2.0 mmI.D. Eluent ： A) water/formic acid (100/0.1)

B) acetonitrile/formic acid (100/0.1)

5% B (0-1 min), 5-100% B (1-5 min), 100% B (5-10 min), 100-5% B (10-10.1 min), 5% B (10.1-12.5 min) Flow rate ：0.4 mL/min

Detection ：ESI positive, TIC (Mass Range: 50-1000)

Triethylamine (pH 11.5, 40 ℃)

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（

ODS

）

YMC-Triart C18

Excellent resolution　－Without adsorption and tailing－

Analysis of basic compounds

Hybrid-silica based C18 Brand I8 5 µm, 150×4.6 mmI.D. Silica based C18 Brand D5 5 µm, 150×4.6 mmI.D. Silica based C18 Brand N1 3 µm, 150×3.0 mmI.D. YMC-Triart C18 5 µm, 150×3.0 mmI.D. 0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min 2 1 3 2 1 3 2 1 3 2 1 3 Q090928C Q090123F Q090821C J091111K

Column ：150×3.0 mmI.D. or 150×4.6 mmI.D.

Flow rate ： 0.425 mL/min for 3.0 mmI.D. 1.0 mL/min for 4.6 mmI.D. Temperature ：40 ℃

Detection ：UV at 235 nm

Ingredients in a cough/cold medication

N N CH3 H3C Cl H3C O O OH O N H CH3 CH3 Chlorpheniramine Propyl p-hydroxybenzoate Dextromethorphan 1. 3. 2.

YMC-Triart C18's innovative surface modification technology results in excellent peak shapes even for the basic compounds that often exhibit tailing shapes on conventional silica and hybrid silica based ODS columns.

Hybrid-silica based C18 Brand I8 5 µm, 150×4.6 mmI.D. Silica based C18 Brand N1 3 µm, 150×3.0 mmI.D. YMC-Triart C18 5 µm, 150×3.0 mmI.D. 0.0 2.0 4.0 6.0 8.0 10.0 12.0 14.0 min 2 1 2 1 2 1 Q090925G Q090120A J091110B

Column ：150×3.0 mmI.D. or 150×4.6 mmI.D. Eluent ：acetonitrile/0.1% H3PO4 (40/60)

Flow rate ： 0.425 mL/min for 3.0 mmI.D. 1.0 mL/min for 4.6 mmI.D. Temperature ：40 ℃ Detection ：UV at 254 nm

Hinokitiol

O O CH3 OH CH3 CH3 O Methyl benzoate Hinokitiol 2. 1.

YMC-Triart C18 has an extremely low level of metal impurities, much lower than conventional products. YMC-Triart C18 is able to provide excellent peak shape for coordination compounds. Hybrid-silica based C18 Brand G4 5 µm, 150×4.6 mmI.D. YMC-Triart C18 5 µm, 150×3.0 mmI.D. 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 min 1 2 3 1 2 3 Q090605C Q090717A

Column ：150×3.0 mmI.D. or 150×4.6 mmI.D. Eluent ：acetonitrile/0.1% H3PO4 (5/95)

Flow rate ： 0.425 mL/min for 3.0 mmI.D. 1.0 mL/min for 4.6 mmI.D. Temperature ：37 ℃

Detection ：UV at 210 nm

YMC-Triart C18 is synthesized utilizing methodology borrowed from microreactor technology. This synthesis technique allows for a reduction of impurities that contribute to peak tailing during the analysis of some types of acidic compounds.

Organic acid

1. Formic acid 2. Acetic acid 3. Propionic acid

Analysis of coordination compounds

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Excellent versatility　－Buffer independency－

Comparison of clemastine analysis with conventional columns

YMC-Triart C18 50×2.0 mmI.D. methanol acetonitrile Silica based C18 Brand C1 50×2.1 mmI.D. Silica based C18 Brand D5 50×2.0 mmI.D. Silica based C18 Brand I6 50×2.1 mmI.D. Hybrid-silica based C18 Brand I8 50×2.1 mmI.D. methanol acetonitrile Tf 0.97 Tf 1.21 Tf 0.93 Tf 1.03 Tf 1.14 Tf 1.11 Tf 1.56 Tf 2.83 Tf 3.87 Tf 1.88 Tf 1.03 Tf 1.13 Tf 1.05 Tf 1.13 Tf 1.22 Tf 1.16 Tf 1.43 Tf 2.21 Tf 1.88 Tf 1.78 10.0 11.0 12.0 min 6.0 7.0 8.0 min 8.0 9.0 10.0 min 6.0 7.0 8.0 min

F090731H07 F090731I07 F090731F07 F090731G07 F090722H07 F090722I07 F090722F07 F090722G07 F090714G07 F090714F07 F090717I07 F090717H07 F090803G07 F090727F07 F090727I07 F090727H07 F090817G07 F090818F07 F090818I07 F090818H07

10 mM phosphate buffer (pH 6.7)/organic solvent 10 mM CH3COONH4/organic solvent

Clemastine is a well known basic compound which can easily tail on conventional ODS columns. YMC-Triart C18 can separate clemastine sharply using many buffer/solvent combinations.

Clemastine

N O

Cl H3C

CH3

Column ：5 µm, 50×2.0 mmI.D. or 50×2.1 mmI.D.

Eluent ： A) 10 mM KH2PO4-K2HPO4 (pH 6.7) or 10 mM CH3COONH4 (pH 6.7)

B) methanol or acetonitrile 5-90% B (0-10 min), 90% B (10-15 min) Flow rate ：0.2 mL/min

Temperature ：25 ℃ Detection ：UV at 230 nm

Improvement of loadability　－Minimizing strong solvent/sample loading effects－

Influence on peak shapes by injection volume

1 2 1 2 min 1.5 2.0 2.5 Hybrid-silica based C18 Silica based C18 YMC-Triart C18 1 ₂ Peak anomaly at 1.5 µL

YMC-Triart C18 can tolerate larger injection volumes of samples containing strong solvents (e.g., acetonitrile) while allowing for better peak shape than traditional columns. This can be important for difficult to solubilize samples that need higher concentrations of organic solvent for solubilization prior to analysis.

Column ：5 µm, 50×2.0 mmI.D. or 2.1 mmI.D. Eluent ： A) water/formic acid (100/0.1)

B) acetonitrile/formic acid (100/0.1) 5% B（0-0.5 min）, 5-100% B (0.5-2.5 min) Flow rate ：0.4 mL/min

Temperature ：40 ℃ Detection ：UV at 275 nm Propranolol (50 µg/mL) Verapamil (50 µg/mL) 1. 2. OCH2CHCH2NHCH(CH3)2 H3CO H3CO CH(CH3)2 CN CH3 OCH3 OCH3 C (CH2)3NCH2CH2 OH acetonitrile 1.0 µL 1.5 µL 2.0 µL 3.0 µL

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ODS

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Versatile Hybrid Silica Based HPLC Column

YMC-Triart C18

YMC-Triart C18 3 µm, 50×2.0 mmI.D.

80% reduction in run time 5%B, 0.4 mL/min, 12.2 MPa 1 2 R091201B mAU 25 20 15 10 5 0 0 1 2 3 4 ₅min Baseline separation, But long analysis time

5%B, 0.2 mL/min, 5.6 MPa 1 2 R091127C mAU 25 20 15 10 5 0 0 5 10 15 20 25 min YMC-Triart C18 5 µm, 150×2.0 mmI.D.

Fast analysis of paraquat and diquat

Insufficient separation 10%B, 0.2 mL/min, 5.7 MPa mAU 25 20 15 10 5 0 0 2 4 6 8 10 12 14 min 1 2 R091126J YMC-Triart C18 5 µm, 150×2.0 mmI.D. Eluent ： A) water/HFBA* (100/0.1) B) acetonitrile/HFBA* (100/0.1) Temperature ：37 ℃ Detection ：UV at 290 nm Injection ：1 µL (0.1 mg/mL) *heptafluorobutyric acid Diquat N+ N+ ·2Br− H3C N+ N+ CH3 ·2Cl− Paraquat 1. 2. Downsizing Eluent optimization Column ： YMC-Triart C18 (5 µm, 120 Å) 250×4.6 mmI.D.

Eluent ： A) water/formic acid (90/10)

B) acetonitrile/methanol/water/formic acid 　 (22.5/22.5/40/10)

20-28% B (0-30 min), 28-70% B (30-40 min), 100% B (40-45 min)

Flow rate ：1.0 mL/min Temperature ：25 ℃

Detection ：UV/VIS at 535 nm

Sample ：commercial bilberry powder (1.25 mg/mL)

1 2 3 4 5 6 7 8 9 10 11 12 13 14 min 0 5 10 15 20 25 30 35 40 mAU 0 20 40 60 80 100 15 16₁₇ 18 19 20

Anthocyanin

1. Delphinidin-3-O-galactoside 2. Delphinidin-3-O-glucoside 3. Cyanidin-3-O-galactoside 4. Delphinidin-3-O-arabinoside 5. Cyanidin-3-O-glucoside 6. Petunidin-3-O-galactoside 7. Cyanidin-3-O-arabinoside 8. Petunidin-3-O-glucoside 9. Peonidin-3-O-galactoside 10. Petunidin-3-O-arabinoside 11. Delphinidin 12. Peonidin-3-O-glucoside 13. Malvidin-3-O-galactoside 14. Peonidin-3-O-arabinoside 15. Malvidin-3-O-glucoside 16. Malvidin-3-O-arabinoside 17. Cyanidin 18. Petunidin 19. Peonidin 20. Malvidin

A p p l i c a t i o n

（Q091110E）

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Reversed-Phase C18 Columns （ ODS ） 0 5 10 15 20 min 0 25 50 75 100 mAU 1 6 5 4 3 2 25

Peptide

1. Oxytocin (MW 1,007) 2. Met-Enkephalin (MW 574) 3. Leu-Enkephalin (MW 556) 4. Neurotensin (MW 1,673) 5. γ-Endorphin (MW 1,859) 6. β-Endorphin (MW 3,465) Column ： YMC-Triart C18 (5 µm, 120 Å) 150×2.0 mmI.D. Eluent ： A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 20-45% B (0-25 min) Flow rate ：0.2 mL/min Temperature ：37 ℃ Detection ：UV at 220 nm

（R091009C）

Ordering information

Analytical columns

Particle size （µm） Pore size （Å） Column size

inner diameter×length（mm） Product number

S-3

120 2.0× 20 TA12S03-0202WT 2.0× 30 TA12S03-0302WT 2.0× 50 TA12S03-0502WT 2.0× 75 TA12S03-L502WT 2.0×100 TA12S03-1002WT 2.0×150 TA12S03-1502WT 3.0× 50 TA12S03-0503WT 3.0×100 TA12S03-1003WT 3.0×150 TA12S03-1503WT 4.6× 35 TA12S03-H546WT 4.6× 50 TA12S03-0546WT 4.6× 75 TA12S03-L546WT 4.6×100 TA12S03-1046WT 4.6×150 TA12S03-1546WT

S-5

120 2.0× 20 TA12S05-0202WT 2.0× 30 TA12S05-0302WT 2.0× 50 TA12S05-0502WT 2.0× 75 TA12S05-L502WT 2.0×100 TA12S05-1002WT 2.0×150 TA12S05-1502WT 3.0× 50 TA12S05-0503WT 3.0× 75 TA12S05-L503WT 3.0×100 TA12S05-1003WT 3.0×150 TA12S05-1503WT 4.6× 50 TA12S05-0546WT 4.6× 75 TA12S05-L546WT 4.6×100 TA12S05-1046WT 4.6×150 TA12S05-1546WT 4.6×250 TA12S05-2546WT

Please inquire for 2 µm columns.

Semi-preparative columns

S-5

120 10×150 TA12S05-1510WT

10×250 TA12S05-2510WT

20×100 TA12S05-1020WT

20×150 TA12S05-1520WT

20×250 TA12S05-2520WT

Semi-preparative columns YMC-Actus Triart C18

S-5

120 20× 50 TA12S05-0520WX

20×100 TA12S05-1020WX

30× 50 TA12S05-0530WX

30× 75 TA12S05-L530WX

30×100 TA12S05-1030WX

See P138-141 for details of YMC-Actus series.

Guard cartridge columns (with inner diameter 2.0 mm: 2/pack, 4.0 mm: 3/pack)

S-3

120 2.0×10 TA12S03-0102CC

4.0×23 TA12S03-G304CC

S-5

120 2.0×10 TA12S05-0102CC

4.0×23 TA12S05-G304CC

A cartridge holder will need to be purchased separately before using this product for the first time.

Cartridge holder

Semi-micro guard cartridge holder

(inner diameter: 1.0, 1.5, or 2.0 mm)

XPCHSMW

Cartridge holder

(inner diameter: 4.0 mm)

XPCHW

Guard columns

S-5

120 10×30 TA12S05-0310WTG 20×50 TA12S05-0520WTG 0 2 4 6 8 10 12 14 Time [min] 0 1 2 3 4 5 6 4 x10 Intens . 100ng_mL_RB4_01_2291.d: EIC 343.051±0.01 +All MS 100ng_mL_RB4_01_2291.d: EIC 343.078±0.01 +All MS Courtesy of J.Watanabe, Bruker Daltonics K. K.

1

2 Mixture 100 ppb

High resolution extracted chromatograms for［M+H］+

LC/MS analysis for Triazolam and Etizolam

Column ： YMC-Triart C18 (5 µm, 120 Å) 50×2.0 mmI.D.

Eluent ： A) 10 mM HCOOH, B) acetonitrile 25-50% B (0-10 min), 25% B (10-15 min) Flow rate ：0.2 mL/min

Detection ：ESI, positive mode (Bruker Daltonics micrOTOF) Injection ：5 µL

（S100121A） Cl N Cl N N C H3 N N Cl S N N C H3 N C H3 Triazolam (MW 343.22) Etizolam (MW 342.85) 1. 2.

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1

（

ODS

）

Ultra-Fast LC column

Characteristics of 2 µm packing material

YMC-UltraHT series shows nearly the same efficiency as sub-2 µm columns for ultra fast LC system and reduces the column pressure significantly.

Interstitial linear velocity (mm/sec)

HETP (mm/plates ) 0.000 0.005 0.010 0.015 0.020 0 1 2 3 4 5 6 7 8 9 10 11 Brand I7 1.7 µm Brand L1 1.8 µm YMC 5 µm YMC 3 µm YMC 2 µm

Optimum ﬂow rate

for 5 µm Optimum ﬂow rate for 2 µm

Van Deemter curves

(0.2 mL/min for 2 mmI.D.) _{(0.3-0.8 mL/min for 2 mmI.D.)}

0 1 2 3 4 5 6 7 8 9 10 11

Interstitial linear velocity (mm/sec)

0 10 20 30 40 50 60 70 80

Column pressure (MPa)

YMC 2 µm YMC 5 µm YMC 3 µm Brand L1 1.8 µm Brand I7 1.7 µm

Column pressure

(0.3-0.8 mL/min for 2 mmI.D.) Optimum ﬂow rate for 2 µm

applicable pressure range for conventional LC Column : 50×2.0 or 2.1 mmI.D. Eluent : acetonitrile/water (60/40) Temperature : 25 ℃

Sample : Butyl benzoate

YMC-UltraHT series

Pro C18 Hydrosphere C18

Particle Size (µm) 2 2

Pore Size (Å) 120 120

Specific Surface area (m2_/g) ₃₃₀ ₃₃₀

Carbon Content 16% 12%

Bonding Type Monomeric Monomeric

Available pH Range 2 - 8 2 - 8

Characteristics Standard type For polar analytes

2 µm ODS column ideal for Ultra-Fast LC

Features

● Superior column performance at higher flow rate and higher pressure ● Reduced analysis time while maintaining excellent resolution

● Excellent resolution with back pressure less than that of sub-2 µm

● Applicable to both conventional HPLC and specific ultra pressure LC system ● Same selectivity and superior peak shapes as 3 µm and 5 µm Pro series ● Simple method transfer from conventional HPLC on Pro series; no need to

change elution conditions

YMC-UltraHT

YMC-UltraHT series is a C18 bonded-phase based on highly efficient 2 µm spherical silica particles. YMC-UltraHT series columns are specifically designed for high-speed and high throughput analysis in various fields such as pharmaceuticals, foods, and environmentals. YMC-UltraHT series has two products. Pro C18 as our

standard ODS, and Hydrosphere C18 for hydrophilic compounds. Both of them show the same selectivity and superior peak shapes as 3 µm and 5 µm Pro series, allowing for an easy method transfer from conventional HPLC to ultra-fast HPLC without changing elution conditions.

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25

1

Ultra Fast analysis for flavonoids

This example shows the method transfer from conventional HPLC method to Ultra-fast LC method using a YMC-UltraHT column. Since the column efficiency is not decreased even when the flow rate is 3 times as fast as the conventional condition, it can be shorten analysis time by 90% without losing the separation characteristics.

conventional LC method ： YMC-Pack C18

150×4.6 mmI.D. 5 µm

tR(7) 24.3 min N(7) 77,000 /m

90% Decrease in analysis time 90% Increase in efficiency tR(7) 2.7 min N(7) 144,000 /m Triple linear Velocity

Ultra-fast LC method ： YMC-UltraHT C18

50×2.0 mmI.D. 2 µm 1.0 mL/min 5 µL injection 8.5 MPa 0.6 mL/min 1 µL injection 30 MPa

Flavonoids

1. Myricetin 2. Quercetin 3. Apigenin 4. Kaempferol 5. Baicalein 6. Chrysin 7. Acacetin

Eluent : acetonitrile/water/formic acid (35/65/0.1)

Temperature : 40 ℃ Detection : UV at 260 nm

Applicable for conventional LC system

Conventional LC system （8 µL, cell length 10 mm） Conventional semi-micro LC system （2.5 µL, cell length 5 mm）

N(1) = 6300 N(2) = 6300 N(3) = 7500 N(4) = 7800 Rs(1, 2) = 2.21 N(1) = 3500 N(2) = 3800 N(3) = 6100 N(4) = 6800 Rs(1, 2) = 1.61 80 60 40 0 mAU 20 0 1 2 3 min 1 2 3 4 (N070606B) 0 1 2 3 min 150 100 50 0 mAU 1 2 3 4 (N070606E)

50

×

3 mmI.D.

Flow rate : 0.8 mL/min Injection : 2 µL

YMC-UltraHT series can be used for fast analysis with conventional LC system, as its pressure is lower than commercial sub-2 µm columns. Considering the extra column band broadening, the column of 3.0 mml.D. is more applicable for conventional LC system than the column of 2.0 mml.D..

Conventional LC system （8 µL, cell length 10 mm） Conventional semi-micro LC system （2.5 µL, cell length 5 mm）

N(1) = 3700 N(2) = 4100 N(3) = 6100 N(4) = 6900 Rs(1, 2) = 1.61 N(1) = 1400 N(2) = 1500 N(3) = 3600 N(4) = 4500 Rs(1, 2) = 0.90 80 60 40 0 mAU 20 0 1 2 3 min 1 2 3 4 (N070608E) 0 1 2 3 min 150 100 50 0 mAU 1 2 3 4 (N070608B)

50

×

2 mmI.D.

Flow rate : 0.36 mL/min Injection : 1 µL

Cephalosporin antibiotics

1. Cephalexin 2. Cefaclor 3. Cephaloglycin 4. Cephaloridine

Column ：YMC-UltraHT Pro C18 Eluent ：acetonitrile/20 mM KH2PO4

　(10/90) Temperature ：37 ℃ Detection ：UV at 260 nm

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1

Analytical columns

YMC-UltraHT

Pro

C18

● Superior column performance at higher flow rate and higher pressure

● Excellent resolution with back pressure less than 60% of commercial sub-2 µm ● Same selectivity and superior peak shapes as 3 µm and 5 µm Pro C18 ● Simple method transfer from conventional HPLC

F i r s t c h o i c e O D S c o l u m n f o r U l t r a - f a s t L C

YMC-UltraHT Pro C18 is a C18 bonded-phase based on highly efficient 2 µm spherical silica particles. YMC-UltraHT Pro C18 is designed for high-speed and high throughput analysis, advanced endcapping technology

makes YMC-UltraHT Pro C18 highly appropriate for analysis of basic compounds. The physical properties and separation characteristics are strictly controlled, so it is highly suitable as the first choice column for ultra fast LC.

■Pore size：120 Å ■Carbon content：16％ ■Usable pH range：2.0 〜 8.0

Excellent peak shapes

N(5) 106,000/m Tf(5) 1.07 30 MPa 1 2 ₃ 4 5 0 60 mV 40 20 0.0 0.4 0.8 1.2 1.6 2.0 [min] YMC-UltraHT C18 50×2.0 mmI.D 2 µm

Alkaloids

1. Scopolamine 2. Atropine 3. Cinchonine 4. Quinine 5. Dihydroquinine Column : 50×2.0 or 2.1 mmI.D.

Eluent : 20 mM CH3COOH-CH3COONH4 (pH 4.9)/

acetonitrile (80/20) Flow rate : 0.6 mL/min Temperature : 40 ℃ Detection : UV at 220 nm sub-2 µm ODS N(5) 89,000/m Tf(5) 3.99 49 MPa 1 2 3 4 5 0 60 mV 40 20 0.0 0.4 0.8 1.2 1.6 2.0 [min] sub-2 µm ODS N(5) 78,000/m Tf(5) 2.35 37 MPa 1 2 ₃ 4 5 0 60 mV 40 20 0.0 0.4 0.8 1.2 1.6 2.0 [min]

YMC-UltraHT Pro C18 exhibits greater peak shapes of alkaloids, which are highly basic compounds compared to commercial silica based sub-2 µm columns.

Peptide mapping

0%B (0-5 min) 0-100%B (5-40 min) 100%B (40-45 min) YMC-Pack C18 150×2.0 mmI.D. 5 µm mV 80 40 0 45 min 30 15 0 (06082204.D) ＊

Eluent ：A) acetonitrile/water/TFA (10/90/0.1) 　B) acetonitrile/water/TFA (35/65/0.1) Flow rate ：0.2 mL/min

Sample ：tryptic degest of Cytochrome c

Analysis of a tryptic digest of cytochrome C is shown below. A 50mm length UltraHT Pro C18 column of 2 µm particle can be shorten the analysis time by one-thirds compared to a 150 mm column packed with 5 µm Pro C18.

*undigested Cytochrome c YMC-UltraHT C18 50×2.0 mmI.D. 2 µm 0%B (0-1.65 min) 0-100%B (1.65-13.35 min) 100%B (13.35-15 min) mV 200 100 0 15 min 10 5 0 （N060811I）＊ same ﬂow rate, column length 1/3x, gradient time 1/3x

Analytical columns

S-2

120 2.0× 30 AS12S02-0302WT 2.0× 50 AS12S02-0502WT 2.0× 75 AS12S02-L502WT 2.0×100 AS12S02-1002WT 3.0× 50 AS12S02-0503WT 3.0× 75 AS12S02-L503WT 3.0×100 AS12S02-1003WT

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1

27

Analytical columns

YMC-UltraHT Hydrosphere C18

● Can be used with 100% aqueous as mobile phase

● Superior column performance at higher flow rate and higher pressure ● Reduced analysis time while maintaining excellent resolution

T h e b e s t c o l u m n f o r h y d r o p h i l i c c o m p o u n d s

YMC-UltraHT Hydrosphere C18 is a C18 bonded phase based on highly efficient 2 µm spherical silica particles.

It is designed to maintain hydrohilicity on the packing surface for superior separation of hydrophilic compounds.

Ultrafast analyses of soy isoflavones

12.5-30% B (0-10 min) 1.5 mL/min 3 µL injection 12.5-30% ACN (0-10 min) 0.3 mL/min 12.5-30% ACN (0-3.3 min) 0.9mL/min

流速3倍、

グラジエント条件最適化

(N070130I) 2 6 0 4 8

10

min

(N060509E) 1 2 3 4 5 6 _{7 8} 9 ₁₀ 11 12 2 6 0 4 8

10

min

1 2 3 4 5 6 ₇ 8 9 ₁₀ 11 12 1 2 0 3

3.3

min

(N070130L) 1 2 3 4 5 6 _{7 8} 9 ₁₀ 11 12 Rs(10,11)=2.38 Rs(7,8)=2.46 Rs(10,11)=2.91 Rs(7,8)=1.75 Rs(10,11)=2.64

粒子サイズ、カラム内径をダウンサイジング

Hydrosphere C18 50×4.6 mmI.D. 3 µm

YMC-UltraHT Hydrosphere C18 2μm 50×2.0 mmI.D.

MPa

Examples illustrated shows the optimization process of isoflavones separation by 2 µm YMC-UltraHT Hydrosphere C18. It has the same selectivity as 3 µm Hydrosphere C18, so there is no need to change the condition at downsizing. Maintaining resolution, the analysis time can be reduced by using 2 µm packing material.

Soy isoflavones

1. Daidzin 2. Glycitin 3. Genistin 4. 6"-O-Malonyldaidzin 5. 6"-O-Malonylglycitin 6. 6"-O-Acetyldaidzin 7. 6"-O-Acetylglycitin 8. 6"-O-Malonylgenistin 9. Daidzein 10. Glycitein 11. 6"-O-Acetylgenistin 12. Genistein 12.5-30% B (0-10 min) 0.3 mL/min 0.6 µL injection (N070130I) 2 6 0 4 8

10

min

1 2 3 4 5 6 ₇ 8 9 ₁₀ 11 12 Rs(10,11)=2.91 YMC-UltraHT Hydrosphere C18 50×2.0 mmI.D.2 µm

12.5-30% B (0-3.3 min)

0.9mL/min 0.6 µL injection YMC-UltraHT Hydrosphere C18 50×2.0 mmI.D. 2 µm

1 2 3 4 5 6 _{7 8} 9 ₁₀ 11 12 (N070130L) Rs(10,11)=2.64 1 2 0 3

3.3

min

downsizing the particle size and column inner diameter

triple ﬂow rate and optimizing the gradient condition

Eluent ：A) water/acetic acid (100/3) 　B) acetonitrile/acetic acid (100/3) Temperature ：35 ℃ Detection ：UV at 254 nm

Analytical columns

S-2

120 2.0× 30 HS12S02-0302WT 2.0× 50 HS12S02-0502WT 2.0× 75 HS12S02-L502WT 2.0×100 HS12S02-1002WT 3.0× 50 HS12S02-0503WT 3.0× 75 HS12S02-L503WT 3.0×100 HS12S02-1003WT

Ordering information

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1

（

ODS

）

High performance reversed-phase column

Features of

Pro

series ODS columns

Pro series high performance reversed-phase columns feature advanced endcapping technology and quality assurance which is ready for validation. The Pro series includes three kinds of ODS, each with unique separation characteristics.

We provide products with excellent performance and stable quality all over the world to fulfill the needs of the pharmaceutical industry, in which production, as well as research and development, are increasingly globalized.

Providing products with stable quality all over the world

Feature

● Silica gel base, low in metal impurities ● Processed with advanced endcapping

technology

● Superior separation of basic compounds ● Useful for LC/MS

● Excellent durability ● Excellent reproducibility

● Two types of test reports are included with each column

YMC-Pack C18 RS is a high carbon "polymeric type" ODS bonding, characterized by high resolution and high durability. It is applicable to a wide range of compounds. The separation of compounds with relatively high hydrophobicity and those that diﬀer only slightly in hydrophobicity, such as structural isomers, is outstanding. It also has excellent resistance to acid and alkali, making it useful under demanding separation conditions.

High carbon ODS column,

useful for separation of highly

hydrophobic compounds

YMC-Pack

Pro

C18 RS

YMC-Pack C18 is a high performance monomeric type ODS column providing standard hydrophobicity, high resolution, high durability and excellent reproducibility. It is highly versatile and suitable as the ﬁrst choice column in almost all ﬁelds, including pharmaceutical products, agricultural chemicals, foods, and natural products.

Standard ODS column

with high versatility

YMC-Pack

Pro

C18

Hydrosphere C18 is an ODS column designed to maintain maximum hydrophilicity without activating the silica surface. It can be used with 100% water eluent, a problematic eluent for conventional ODS columns. Hydrosphere C18 is useful for separating hydrophilic compounds.

Low carbon ODS column,

useful for separation of

hydrophilic compounds

Hydrosphere C18

Types of Pro series ODS

Pro C18 Hydrosphere C18 Pro C18 RS

Particle Size (µm) 2 , 3 , 5 , 10 2 , 3 , 5 3 , 5

Pore Size (Å) 120 120 80

Specific Surface area (m2_/g) ₃₃₀ ₃₃₀ ₅₁₀

Carbon Content 16% 12% 22%

Bonding type monomeric monomeric polymeric

Available pH Range 2 - 8 2 - 8 1 - 10

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29

1

The three kinds of ODS columns in the Pro series each have unique carbon content and separation characteristics, including hydrophobicity, plane recognition ability, and hydrogen-bonding capacity. Selection according to the analyte and mobile phase of Pro C18 with standard hydrophobicity, Pro C18 RS with high hydrophobicity, or Hydrosphere C18 with low hydrophobicity, facilitates method development.

Three kinds of ODS, each with different separation characteristics

Hydrophobicity and plane recognition ability of Pro series ODS

5 10 15 20 25 0 min J020805E 1 2 3 4 5 YMC-Pack C18 RS 1. Uracil 2. n-Butylbenzene 3. o-Terphenyl 4. n-Amylbenzene 5. Triphenylene

Column size ：150×4.6 mmI.D. Eluent ：methanol/water (80/20) Flow rate ：1.0 mL/min

Temperature ：37 ℃ Detection ：UV at 254 nm 5 10 15 20 0 min J020805B 1 2 3 4 5 YMC-Pack C18 5 10 15 20 0 min J020805D 1 2 3 4 5 Hydrosphere C18

Comparison of hydrophobicity and hydrogen-bonding capacity of various columns

XTerra MS C18 (S090805A) Gemini NX-C18 2.00 4.00 6.00 8.00 10.00 12.00 14.00

Hydr

ogen-bonding capacity

α

(ca

ffeine/benzene

)

Hydrophobicity k’ (amylbenzene)

Inertsil ODS-4

Eluent : MeOH/W (80/20) [amylbenzene], MeOH/W (30/70) [caffeine, benzene]

Pro

C18 RS

Develosil ODS-SR Develosil C30-UG Atlantis T3

Hydrosphere C18

CAPCELL PAK C18 AQ

Pro

C18

Gemini C18 Hypersil GOLD XBridge C18 Eternity-C18 Cadenza CD-C18 L-column2 ODS TSKgel ODS-100S L-column ODS Mightysil RP-18 SunFire C18 Inertsil ODS-3 CAPCELL PAK C18 MGII CAPCELL PAK C18 MGIII

Unison UK-C18 0.040 0.060 0.080 0.100 0.120 0.140 0.160 0.180 Hydrophobicity: low

Hydrogen-bonding capacity: high

Hydrophobicity: intermediate Hydrogen-bonding capacity: middle

Hydrophobicity: high Hydrogen-bonding capacity: low

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1

（

ODS

）

High performance reversed-phase column

Features of

Pro

series ODS columns

Comparison of separation behavior on the three kinds of ODS columns in the Pro series

Column ：150×4.6 mmI.D. Eluent ：methanol/water (55/45) Flow rate ：1.0 mL/min

Parabenzoates

3 types of ODS are available to separate the para-benzoates shown in the left, but the best separation between peaks 5 and 6 is achieved on Pro C18 RS. 1 2 3 4 6 5 10 20 30 0 min （J020827E） C18 RS 1 2 3 4 6 5 10 20 0 min （J020827D） C18 1 2 3 4 6 5 10 20 0 min （J020827C） Hydrosphere C18 Methyl p-hydroxybenzoate OH COOCH3 Ethyl p-hydroxybenzoate Propyl p-hydroxybenzoate Butyl p-hydroxybenzoate Isopropyl p-hydroxybenzoate Isobutyl p-hydroxybenzoate COOCH CH3 CH3 OH OH COOCH2CH3 COO(CH2)2CH3 OH COOCH2CH CH3 CH3 OH COO(CH2)3CH3 OH 1. 2. 3. 4. 5. 6.

Flavonoid glycosides

C18 RS C18 Hydrosphere C18 5 10 15 0 min 1 2 3 J020828F 4 1 2 3 4 J020828A 1 2 3 4 J020828C O O HO OH HO OR HO Hydrogen-bonding interactions significantly contribute to the retention of hydrophilic compounds, including glycosides. Therefore, the retention is longer in the Hydrosphere C18, which has strong hydrogen-bonding capacity, than in Pro C18 RS, which has highly hydrophobic packing materials. To separate such hydrophilic compounds, the Hydrosphere C18 is suitable for

precise separation of multicomponent samples, while Pro C18 RS and Pro C18 are suitable for rapid separation of simple components.

Column ：150×4.6 mmI.D.

Eluent ：acetonitrile/10 mM H3PO4 (25/75)

Flow rate ：1.0 mL/min Temperature ：40 ℃ Detection ：UV at 260 nm 1. Rutin 2. Isoquercitrin 3. Quercitrin 4. Quercetin R = rutinose R = glucose R = rhamnose R = H

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31

1

Terphenyl isomers

1 2 3 10 20 0_J020719E min C18 RS 1. 2. 3. m-Terphenyl o-Terphenyl p-Terphenyl

Since Pro C18 RS has excellent hydrophobic selectivity and shape selectivity, it works well in the separation of structural isomers that are not easily separated by other ODS.

Column ：150×4.6 mmI.D. Eluent ：methanol/water

　(85/15) Flow rate ：1.0 mL/min Temperature ：37 ℃ Detection ：UV at 254 nm 1 2 3 10 20 0_J020719J min C18 1 2 3 10 20 0 min J020722A Hydrosphere C18 10 20 0_J020719K min Brand C1 1 2,3

Saikosaponins

C18 Hydrosphere C18 C18 RS Brand B2 10 20 30 40 0 F030224E 1 23 4 4 4 1 1 2,3 2,3 F030222C F030222A min 1 23 4 F030222B

Peaks 2 and 3, which are not easily separated by other ODS, can be completely separated by Hydrosphere C18. It is thought that hydrogen-bonding interaction of hydrophilic functional groups in sugar chain and terpene skeleton as well as hydrophobic interaction has great contribution to the separation of saikosaponins.

Hydrosphere C18 is the most appropriate in the separation of this kind of class of compound.

O glucose glucose6 4 rhamnose O H OH Saikosaponin c O fucose glucose3 O H OH CH2OH Saikosaponin a Saikosaponin b2 O fucose glucose3 O H OH CH2OH Saikosaponin d 1. 2. 3. 4. fucose glucose₃ O OH CH2OH CH2OH Column ：150×4.6 mmI.D. Eluent ：acetonitrile/water (38/62) Flow rate ：1.0 mL/min

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1

Analytical columns

YMC-Pack

Pro

C18

● Superior separation of basic compounds

● Processed with advanced endcapping technology

● Excellent reproducibility

● Utilizes highly pure silica gel base ● Two types of test reports are included

with each column

P e r f e c t l y e n d c a p p e d O D S

YMC-Pack Pro C18 includes more advanced endcapping technology for strictly controlled processing of residual silanol groups that are likely to affect quality. Pro C18 is highly appropriate for basic compounds, including pharmaceutical products and agricultural chemicals.

Two test reports are included with each product; a Column Test Report, indicating the column performance, and a Packing Material Test Report, detailing strictly controlled physical properties and separation characteristics of the packing material.

Useful for separation of basic compounds

Column ：YMC-Pack Pro C18 (5 µm, 120 Å) 　150×4.6 mmI.D.

Eluent ：20 mM KH2PO4-K2HPO4 (pH6.9)/

　methanol (25/75) Flow rate ：1.0 mL/min Temperature ：37 ℃ Detection ：UV at 254 nm

Tricyclic antidepressants

（A961015G）0 10 20min 1 2 3

4 Advanced endcapping technology _{results in good peak shapes even}

for basic compounds that often elute with poor tailing shapes on competitive columns. CHCH2CH2N CH_CH3 3 HCI ・ N CH2CH2CH2N CH2CH2CH2NHCH3 CH3 CH3 HCI ・ N HCI ・ 1. Desipramine hydrochloride 2. Acenaphthene(I.S.) 3. Imipramine hydrochloride 4. Amitriptyline hydrochloride

Basic peptides

（A990123B） 1 2 3 4 56 0 5 10 15 20 min C18

YMC-Pack Pro C18 is processed using advanced endcapping technology and produces a better chromatogram than that obtained using ODS-A for separating basic peptides. Similar chromatograms have been obtained with YMC-Pack Pro C18 and ODS-A for separation of neutral peptides.

（A990121A） 1 2 3 4 5 6 0 5 10 15 20 min ODS-A

Neutral peptides

（A990121B）0 5 10 15 20 min 1 2 3 4 5 ₆ C18 （A990119B）0 5 10 15 20 min 1 2 3 4 5 6 ODS-A 1. Bradykinin 2. Dynorphin A (Human,1-13) 3. Eledoisin related peptide 4. Neurotensin

5. Bombesin 6. Substance P

Column ：YMC-Pack Pro C18 and ODS-A (5 µm, 120 Å) 　75×4.6 mmI.D.

Eluent ：A) water/TFA (100/0.1) 　B) acetonitrile/TFA (100/0.1) 　20-40％ B (0-20 min,linear) Flow rate ：1.0 mL/min

Temperature ：37 ℃ Detection ：UV at 220 nm 1. Oxytocin 2. Met-Enkephalin 3. Leu-Enkephalin 4. Angiotensin l 5. α-Mating factor 6. Insulin

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1

Excellent lot-to-lot reproducibility

batch-to-batch

0 4 8 min Lot C Lot B Lot A Test B 1. Caffeine 2. Pyridine 3. Phenol

A three-lot comparison is shown on the left. Excellent reproducibility is achieved in separating hydrophilic, basic and acidic compounds, as well as hydrophobic compounds. 0 5 10 15 min Lot C Lot B Lot A Test A 1. Uracil 2. 4-n-Butoxybenzoic acid 3. Ethylbenzene 4. n-Propylbenzene

column-to-column

Advanced packing technology results in high column efficiency. All packed columns are inspected and controlled according to the strictest quality control criteria for column performance ever, including theoretical plate number testing. 24000 21000 18000 15000 12000 9000 6000 3000 20 40 60 80 100 Column No.

Theoretical plate number

150×4.6 mml.D. theoretical plate number

Two types of test reports

〈Packing Material Test Report〉

Each YMC-Pack Pro C18 includes both, a Column Test Report and a Packing Material Test Report, to certify lot-to-lot and column-to-column reproducibility.

〈Column Test Report〉

This report describes basic physical properties of silica gel, including particle size, pore size and metal content, carbon loading after modification, and the specifications and results of separation characteristics tests.

The Column Test Report shows the theoretical plate number and asymmetry factor to indicate column performance. For convenience in deterioration evaluation, this report includes all analytical conditions including sample concentration as well.

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1

Analytical columns

YMC-Pack

Pro

C18

1 2 3 4 5 6 min 0 5 10 15 20 F040119B C18 Column ：150×4.6 mmI.D. Eluent ：A) 20 mM KH2PO4-H3PO4 (pH 2.5) 　B) methanol 　20-90% B (0-15 min), 90% B (15-20 min) Flow rate ：1.0 mL/min

Temperature ：37 ℃ Detection ：UV at 260 nm 1 ₂ 3 4 5 6 min 0 5 10 15 20 F030130C Brand I4 min 0 5 10 15 20 F040130E 1 2 3 4, 5 6 Brand L1 Naphazoline Chlorpheniramine

Methyl p-hydroxybenzoate Benzethonium chloride Lidocaine Maleic acid 1 2 3 4 5 6 CHCOOH CHCOOH CH3_NHCCH2O _N(CH2_CH3)2 CH3 N CH2 N H CHCH2CH2N CH3 CH3 N Cl OH COOCH3 NCH2 CH3 CH3 (OCH2CH2)2 + Cl -C CH3 CH3 CH2 C CH3 CH3 CH3

nasal drops (OTC)

2 3 ₄ 5 1 0 10 20 min

catechins

1. (-)-Epigallocatechin 2. (+)-Catechin 3. (-)-Epicatechin 4. (-)-Epigallocatechin gallate 5. (-)-Epicatechin gallate

Column : YMC-Pack Pro C18 (5 µm, 120 Å) 150×4.6 mmI.D.

Eluent : acetonitrile/ethyl acetate/water/TFA (9/3/88/0.1)

Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 280 nm 1 ₂ 3 4 6 ₇ 5 0 5 10 15 min

antioxidants

1. n-Propyl gallate(PG) 2. 2,4,5-Trihydroxybutyrophenone (THBP) 3. tert-Butylhydroquinone(TBHQ) 4. Nordihydroguaiaretic acid (NDGA)

5. 4-Hydroxymethyl-2,6-di-tert-butylphenol (HMBP) 6. n-Octyl gallate(OG)

7. n-Dodecyl gallate(DG)

Column ：YMC-Pack Pro C18 (5 µm, 120 Å) 　75×4.6 mm I.D.

Eluent ：A) acetonitrile/methanol/5% acetic acid (20/20/60) 　B) acetonitrile/methanol/5% acetic acid (45/45/10) 　0-100% B (0-5 min), 100% B (5-15 min)

（N050620A）

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35

1

Analytical columns

S-3

120 1.0× 35 AS12S03-H501WT 1.0× 50 AS12S03-0501WT 1.0×100 AS12S03-1001WT 1.5× 35 AS12S03-H5P5WT 1.5× 50 AS12S03-05P5WT 1.5×100 AS12S03-10P5WT 2.0× 35 AS12S03-H502WT 2.0× 50 AS12S03-0502WT 2.0× 75 AS12S03-L502WT 2.0×100 AS12S03-1002WT 2.0×150 AS12S03-1502WT 3.0× 50 AS12S03-0503WT 3.0×100 AS12S03-1003WT 3.0×150 AS12S03-1503WT 4.6× 35 AS12S03-H546WT 4.6× 50 AS12S03-0546WT 4.6× 75 AS12S03-L546WT 4.6×100 AS12S03-1046WT 4.6×150 AS12S03-1546WT 6.0× 50 AS12S03-0506WT 6.0× 75 AS12S03-L506WT 6.0×100 AS12S03-1006WT

S-5

120 1.0×150 AS12S05-1501WT 1.0×250 AS12S05-2501WT 1.5×150 AS12S05-15P5WT 1.5×250 AS12S05-25P5WT 2.0× 35 AS12S05-H502WT 2.0× 50 AS12S05-0502WT 2.0× 75 AS12S05-L502WT 2.0×100 AS12S05-1002WT 2.0×150 AS12S05-1502WT 2.0×250 AS12S05-2502WT 3.0× 50 AS12S05-0503WT 3.0× 75 AS12S05-L503WT 3.0×100 AS12S05-1003WT 3.0×150 AS12S05-1503WT 3.0×250 AS12S05-2503WT 4.6× 50 AS12S05-0546WT 4.6× 75 AS12S05-L546WT 4.6×100 AS12S05-1046WT 4.6×150 AS12S05-1546WT 4.6×250 AS12S05-2546WT 6.0×150 AS12S05-1506WT 6.0×250 AS12S05-2506WT

S-10

120 4.6×150 AS12S11-1546WT 4.6×250 AS12S11-2546WT

S-5

120 10×150 AS12S05-1510WT 10×250 AS12S05-2510WT 20×100 AS12S05-1020WT 20×150 AS12S05-1520WT 20×250 AS12S05-2520WT

S-10

120 20×100 AS12S11-1020WT 20×150 AS12S11-1520WT 20×250 AS12S11-2520WT

See P142 for preparative columns other than those listed above.

Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)

S-3

120 1.0×10 AS12S03-0101CC 1.5×10 AS12S03-01P5CC 2.0×10 AS12S03-0102CC 4.0×23 AS12S03-G304CC

S-5

120 1.0×10 AS12S05-0101CC 1.5×10 AS12S05-01P5CC 2.0×10 AS12S05-0102CC 4.0×23 AS12S05-G304CC

A guard cartridge holder will need to be purchased separately before using this product for the first time.

Cartridge holder

XPCHSMW

Cartridge holder

XPCHW

YMC guard cartridge pack

S-3

120 4.0×23 AS12S03-G304CCPK

S-5

120 4.0×23 AS12S05-G304CCPK

A guard cartridge pack includes a cartridge holder (1 set), a guard cartridge column, and a column coupler (produced by PEEK).

Guard columns

S-5

120 10×30 AS12S05-0310WTG 20×50 AS12S05-0520WTG

S-10

120 20×50 AS12S11-0520WTG

When columns packed with material from different lots are needed for validation purposes, please contact us.

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1

Analytical columns

Hydrosphere C18

● Strong retention of hydrophilic compounds ● Can be used with 100% water mobile phase ● Superior separation of basic compounds ● Excellent reproducibility

● Utilizes highly pure silica gel base ● Two types of test reports are included with

each column

H y d r o p h i l i c O D S

Hydrosphere C18 is designed to maintain adequate hydrophilicity on the packing surface for superior separation of hydrophilic compounds. Hydrophilic compounds are retained with much greater affinity than with conventional ODS columns, permitting the product to be used

with 100% water eluent, a problematic eluent for conventional ODS columns. Hydrosphere C18 is useful for separating a wide range of compounds, including nucleic acids and their derivatives, organic acids, saccharides, glycosides and peptides.

Appropriate for separation of hydrophilic compounds

Separation of organic acids

0 5 10 min A000310E 1 2 34 5 Hydrosphere C18

High hydrophilicity of the Hydrosphere C18 packing material surface enables longer retention and superior separation of citric acid and fumaric acid as compared to a conventional ODS column (Brand Ⅰ). 1 2 3,4 5 5 10 0 min A000615A

conventional ODS column （Brand Ⅰ）

1. Lactic acid 2. Acetic acid 3. Citric acid 4. Fumaric acid 5. Succinic acid Column ：150×4.6 mm I.D. Eluent ：20 mM NaH2PO4-H3PO4 (pH 2.8)

Reproducibility of retention time when used with 100% water mobile phase

S0515A 10 20 0 min ※ 2 1 3 4 5 Hydrosphere C18

When conventional ODS columns are used with 100% water mobile phase, the apparent hydrophobicity decreases due to repulsive interactions between water and hydrophobic groups on the surface of the packing materials, reducing retention of compounds. In the experiment illustrated here, columns filled with 100% water mobile phase were left to stand overnight. Retention times of nucleic acid bases were analyzed before and after standing and compared. The retention times of adenine after standing decreased to 89% of the original value for Brand II and to 71% of the original Brand III value. By contrast, Hydrosphere C18 retained 98% of its original retention for adenine. Hydrosphere C18 is designed to maintain adequate hydrophilicity on the packing surface so that hydration can be achieved, and when Hydrosphere C18 is used with mobile phase containing no organic solvent, the retention time is not significantly shortened and highly reproducible chromatograms can be obtained.

S0518H 10 20 0 min ※ 2 1 3 4 5

conventional ODS column （Brand Ⅱ）

S0518F 10 20 0 min ※ 2 1 3 4 5

conventional ODS column （Brand Ⅲ）

S0517A ※ ₉₈_％ 10 20 0 min 89％ S0519A ※ 10 20 0 min 71％ S0519C ※ 10 20 0 min 1. Cytosine 2. Uracil 3. Guanine 4. Thymine 5. Adenine Column ：150×4.6 mm I.D. Eluent ：20 mM KH2PO4-K2HPO4 (pH 6.9)

Flow rate ：1.0 mL/min Temperature ：37 ℃ Detection ：UV at 254 nm A ft er s ta nd in g Be fo re s ta nd in g

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37

1

Differences in separation characteristics from conventional ODS columns

0 10 20 30 40 min S000309A 1 2 3 4 5 6 Hydrosphere C18

The chromatograms shown were obtained in tests conducted to compare separation characteristics using a mixed sample containing compounds with different characteristics.

The packing material surface of Hydrosphere C18 has higher hydrophilicity and lower C18 ligand density than those of Pro C18. This results in shorter retention of hydrophobic compounds, including n-propylbenzene and n-butylbenzene, and planar compounds like acenaphthene. By contrast, retention of testosterone, a compound with large hydrogen-bonding properties, is longer on Hydrosphere C18 and on Brand IV, which also has a highly hydrophilic packing surface.

Amitriptyline, a basic compound, caused tailing peaks in Brand IV and Brand V. By contrast, Hydrosphere C18 and Pro C18 produce good peak shapes for such basic compounds. Although the retention times of amitriptyline on Hydrosphere C18 and Pro 18 are similar, there is a big difference between the two columns with regard to resolution of n-butylbenzene and amitriptyline. This difference is considered to be due to the hydrophilicity of the Hydrosphere C18 surface.

These results demonstrate that Hydrosphere C18, having different separation characteristics from conventional ODS columns, enables separation of compounds that cannot be separated using other ODS columns.

0 10 20 30 40 min 1 2 3 4 5 6 S990607B C18 10 20 30 40 50 60 0 min F000614B 1 2 3 4 5 6 Brand Ⅳ 10 20 30 40 50 60 70 80 90 0 min F000614C 1 2 3 4 5 6 Brand Ⅴ 1. Toluene 2. Testosterone 3. n-Propylbenzene 4. Acenaphthene 5. n-Butylbenzene 6. Amitriptyline hydrochloride Column ：150×4.6 mm I.D.

Eluent ：20 mM KH2PO4-K2HPO4 (pH 6.9)/methanol (35/65)

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1

Analytical columns

Hydrosphere C18

5 10 15 20 25 0 min 1 2 3 4 5 6

Nicotinic acid and derivatives

1. Nicotinamide adenine dinucleotide phosphate oxidized form (NADP) 2. Nicotinic acid

3. Nicotinamide adenine dinucleotide phosphate reduced form (NADPH) 4. Nicotinamide

5. β-Nicotinamide adenine dinucleotide (NAD)

6. β-Nicotinamide adenine dinucleotide reduced form (NADH) Column ：Hydrosphere C18 (5 µm, 120 Å)

　150×4.6 mm I.D.

Flow rate ：1.0 mL/min Temperature ：37 ℃ Detection ：UV at 260 nm　

（F000707B）

Short analysis time

Column ：50×4.6 mm I.D. (3 µm, 120 Å)

Eluent ：acetonitrile/methanol/5% acetic acid (35/35/30) Flow rate ：1.0 mL/min

Hydrosphere C18 can reduce the time necessary for analyzing compounds with high hydrophobicity by about 20% as compared with conventional ODS columns. 5 10 15 0 min J010216B 1 3 4 5 2 6

conventional ODS column

5 10 15 0 min J010215B 1 3 4 ₅ 2 6 Hydrosphere C18

Antioxidants

1. Nordihydroguaiaretic acid (NDGA) 2. Butyl hydroxyanisol (BHA)

3. 4-Hydroxy-2,6-di-tert-butylphenol (HMBP) 4. n-Octyl gallate (OG)

5. Dibutyl hydroxytoluene (BHT) 6. n-Dodesyl gallate (DG) 1 2 3 4 6 7 5 5 10 15 20 0 min 8 9 11 10

Nucleotides

1. 5’-UTP 7. 5’-ATP 2. 5’-UDP 8. 5’-IMP 3. 5’-CMP 9. 5’-ADP 4. 5’-UMP 10. 5’-TMP 5. 5’-dCMP 11. 5’-AMP 6. 5’-GMP Column ：Hydrosphere C18 (5 µm, 120 Å) 　150×4.6 mm I.D. Eluent ：100 mM KH2PO4-K2HPO4 (pH 5.5)

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39

1

Analytical columns

S-3

120 1.0× 35 HS12S03-H501WT 1.0× 50 HS12S03-0501WT 1.0×100 HS12S03-1001WT 1.5× 35 HS12S03-H5P5WT 1.5× 50 HS12S03-05P5WT 1.5×100 HS12S03-10P5WT 2.0× 35 HS12S03-H502WT 2.0× 50 HS12S03-0502WT 2.0× 75 HS12S03-L502WT 2.0×100 HS12S03-1002WT 2.0×150 HS12S03-1502WT 3.0× 50 HS12S03-0503WT 3.0×100 HS12S03-1003WT 3.0×150 HS12S03-1503WT 4.6× 35 HS12S03-H546WT 4.6× 50 HS12S03-0546WT 4.6× 75 HS12S03-L546WT 4.6×100 HS12S03-1046WT 4.6×150 HS12S03-1546WT 6.0× 50 HS12S03-0506WT 6.0× 75 HS12S03-L506WT 6.0×100 HS12S03-1006WT

S-5

120 1.0×150 HS12S05-1501WT 1.0×250 HS12S05-2501WT 1.5×150 HS12S05-15P5WT 1.5×250 HS12S05-25P5WT 2.0× 35 HS12S05-H502WT 2.0× 50 HS12S05-0502WT 2.0× 75 HS12S05-L502WT 2.0×100 HS12S05-1002WT 2.0×150 HS12S05-1502WT 2.0×250 HS12S05-2502WT 3.0× 50 HS12S05-0503WT 3.0× 75 HS12S05-L503WT 3.0×100 HS12S05-1003WT 3.0×150 HS12S05-1503WT 3.0×250 HS12S05-2503WT 4.6× 50 HS12S05-0546WT 4.6× 75 HS12S05-L546WT 4.6×100 HS12S05-1046WT 4.6×150 HS12S05-1546WT 4.6×250 HS12S05-2546WT 6.0×150 HS12S05-1506WT 6.0×250 HS12S05-2506WT

S-5

120 10×150 HS12S05-1510WT

10×250 HS12S05-2510WT

20×100 HS12S05-1020WT

20×150 HS12S05-1520WT

20×250 HS12S05-2520WT

Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)

S-3

120 1.0×10 HS12S03-0101CC 1.5×10 HS12S03-01P5CC 2.0×10 HS12S03-0102CC 4.0×23 HS12S03-G304CC

S-5

120 1.0×10 HS12S05-0101CC 1.5×10 HS12S05-01P5CC 2.0×10 HS12S05-0102CC 4.0×23 HS12S05-G304CC

A guard cartridge holder will need to be purchased separately before using this product for the first time.

Cartridge holder

XPCHSMW

Cartridge holder

XPCHW

YMC guard cartridge pack

S-3

120 4.0×23 HS12S03-G304CCPK

S-5

120 4.0×23 HS12S05-G304CCPK

A guard cartridge pack includes a cartridge holder (1 set), a guard cartridge column, and a column coupler (produced by PEEK).

Guard columns

S-5

120 10×30 HS12S05-0310WTG 20×50 HS12S05-0520WTG

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1

Analytical columns

YMC-Pack

Pro

C18 RS

● Excellent acid resistance and alkali resistance (pH 1 to 10)

● Superior separation of structural isomers ● Superior separation of basic compounds

● Excellent reproducibility ● Utilizes highly pure silica gel base ● Two types of test reports are included

with each column

H i g h l y d u r a b l e O D S

YMC-Pack Pro C18 RS is a polymeric type high carbon ODS column characterized by high resolution and high durability. It is applicable to a wide range of compounds, providing good separation of basic compounds that easily cause

tailing peaks. The separation selectivity for compounds that differ only slightly in hydrophobicity is outstanding. It also has excellent resistance to acid and alkali, making it useful under demanding separation conditions.

Excellent durability

0 20 40 60 80 100 0 20 40 60 80 100(hours) YMC-Pack Pro C18 RS Acid-resistant column (manufactured by other company) Monomeric ODS

Hours exposure to acetonitrile/water/TFA (10/90/1), change in packing material hydrophobicity at 70 ℃.

%

The C18 RS packing material hydrophobicity is hardly changed under the harsh conditions of pH1 and column temperature 70 ℃.

pH 1 0 20 40 60 80 100 0 200 400 600 800(hours) Pro C18 RS Monomeric ODS Sequential analysis using 20 mM H3BO3-NaOH (pH 9.8)/methanol (50/50) % (

)

.

Unlike conventional ODS columns, C18 RS can be used with an alkali mobile phase.

pH 10

Pro C18 RS with excellent acid and alkali resistance can be used over a wide pH range, and it is easy to optimize the separating conditions. In addition, by downsizing from the column (particle 5 µm, length 150 mm) to a shorter column (particle 3 µm, length 50 mm), with high separation ability, it becomes possible to shorten the analysis time by one third without affecting the separation.

1. Flurbiprofen 2. Methyl benzoate 3. Lidocaine 4. Diltiazem 5. Verapamil

Column ：YMC-Pack Pro C18 RS

Eluent ：20 mM acetate buffer/methanol (35/65) Flow rate ：1.0 mL/min

A wide choice of mobile phases facilitates method development

0 5 10 15 20 min 0 5 10 15 20 min F040714E F040716A 3,4,5 1 2 ₁2 3 5 4 0 5 10 15 20 min F040714C 2 1 3 5 4 150×4.6 mmI.D. 5 µm pH 3.2 pH 6.8 pH 8.8 0 5 10 min F040714D 2 1 3 5 4 50×4.6 mmI.D. 3 µm pH 8.8 Downsizing

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41

1

Separation of basic compounds

Amitriptyline is a basic compound that can easily tail on some silica based columns. In comparison with competing acid-resistant columns, Pro C18 RS produces good peak shapes without tailing.

Tf(4) 1.16 10 20 0 min J011114B 1 2 3 ₄ YMC-Pack C18 RS Tf(4) 2.87 Acid-resistant column manufactured

by other company（Brand D3） 1 2 3 ₄ 10 20 0 min J020523D 1. Desipramine 2. Diphenyl (I.S.) 3. Imipramine 4. Amitriptyline Column ：150×4.6 mmI.D. Eluent ：20 mM KH2PO4-K2HPO4 (pH 6.9) /methanol (25/75) Flow rate ：1.0 mL/min Temperature ：37 ℃ Detection ：UV at 254 nm

Separation of structural isomers

Pro C18 RS with a high C18 density is excellent in hydrophobicity recognition ability and plane recognition ability, and can be applied to separate structural isomers which are difficult to separate with other ODS. In addition, by using 3 µm column packing, resolution can be increased, and it becomes possible to achieve a baseline separation of linolenate isomers.

F040713D 0 10 20 30min 2 1 Brand F1 (standard-type ODS 5 µm) Rs=0.8 F040714 0 10 20 30 min 2 1 Rs=1.24 C18 RS 5 µm

Methyl linolenate isomers

1. Methyl linolenate

2. Methyl γ-linolenate

Column ：150×4.6 mmI.D.

Eluent ：acetonitrile/THF/water (35/30/35) Flow rate ：1.0 mL/min

Temperature ：30 ℃ Detection ：UV at 210 nm F040713A 0 10 20 30 min 2 1 C18 RS 3 µm Rs=1.54

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1

Analytical columns

YMC-Pack

Pro

C18 RS

Separation of peptides by LC/MS

400 600 800 1000 1200(m/z) 0 100 % 530.95 531.47 1060.27 5.00 10.00 15.00 20.00（Time） 18 100 % 1 2 3 4 5 ₆ Bradykinin(MW: 1059.56) M+2 M+1

Pro C18 RS produces good peak shapes when it is used with mobile phase containing low TFA concentration, making it suitable for LC/ MS. 1. [Arg8_{]-Vasopressin} 2. Bradykinin 3. Angiotensin Ⅱ 4. Oxytocin 5. Angiotensin Ⅰ 6. Leu-Enkephalin

Column ：YMC-Pack Pro C18 RS (5 µm, 80 Å) 　150×2.0 mmI.D.

Eluent ：A) water/TFA (100/0.01) 　B) acetonitrile/TFA (100/0.01) 　10-35% B (0-15 min) Flow rate ：0.2 mL/min Temperature ：37 ℃

Detection ：ESI positive-mode

1 2 3 10 20 0 min J011026B

Eluent ： acetonitrile/water/acetic acid (30/70/1)

Chloroacetophenone isomers

10 20 0 min J011113D 1 2 3

Chlorophenol isomers

Eluent ：acetonitrile/water (40/60) Flow rate ：1.0 mL/min

Temperature ：30 ℃ Detection ：UV at 254 nm 1. 2. 3. m-Chloroacetophenone COCH3 Cl o-Chloroacetophenone COCH3 Cl COCH3 Cl p-Chloroacetophenone 1. 2. 3. m-Chlorophenol o-Chlorophenol p-Chlorophenol OH Cl OH Cl OH Cl

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43

1

Analytic

1 Reversed-Phase C18 Columns (ODS)

YMC GENERAL CATALOG

Reversed-Phase

YMC-Pack

Base Products

Hybrid silica

Product name Pore size

ODS-AQ

Types and characteristics of C18 packing materials

Chemical structures diﬀer

YMC-Triart C18

YMC-Triart C18 is a hybrid silica based ODS column emphasizing durability, low operating pressure, and excellent performance. YMC-Triart C18 has been developed and is manufactured using state-of-the-art technologies that define materials, granulation and surface modification.

YMC-Triart C18 －Integration of triple artistic technologies－

Image structure for hybrid-silica gel

Particle

Features

● Hybrid silica base ▼ Usable over a wide pH range makes the column excellent for method development.

Narrow pore distribution

Triart C18

YMC-Triart C18 －Integration of triple artistic technologies－

＜3＞Surface modification technology

STEP STEP

Evaluation of silanol activity

Packing Material

Low back-pressure －Free from pressure problem－

Standard ODS －Best balance hydrophobicity－

CAPCELL PAK C18 MGII CAPCELL PAK C18 MGIII

Coordination compounds

YMC-Triart C18

YMC-Triart C18

Reduced column bleed －Operate at high sensitivity－

Triethylamine (pH 11.5, 40 ℃)

Ingredients in a cough/cold medication

Hinokitiol

Organic acid

Improvement of loadability －Minimizing strong solvent/sample loading effects－

Versatile Hybrid Silica Based HPLC Column

Anthocyanin

Guard cartridge columns (with inner diameter 2.0 mm: 2/pack, 4.0 mm: 3/pack)

LC/MS analysis for Triazolam and Etizolam

Van Deemter curves

Pro C18 Hydrosphere C18

2 µm ODS column ideal for Ultra-Fast LC

● Superior column performance at higher flow rate and higher pressure ● Reduced analysis time while maintaining excellent resolution

YMC-UltraHT

Ultra Fast analysis for flavonoids

tR(7) 24.3 min N(7) 77,000 /m

Flavonoids

Cephalosporin antibiotics

● Superior column performance at higher flow rate and higher pressure

Excellent peak shapes

Peptide mapping

Analytical columns

● Superior column performance at higher flow rate and higher pressure ● Reduced analysis time while maintaining excellent resolution

Ultrafast analyses of soy isoflavones

Soy isoflavones

Ordering information

Pro series high performance reversed-phase columns feature advanced endcapping technology and quality assurance which is ready for validation. The Pro series includes three kinds of ODS, each with unique separation characteristics.

Providing products with stable quality all over the world

● Silica gel base, low in metal impurities ● Processed with advanced endcapping

High carbon ODS column,

Standard ODS column

Low carbon ODS column,

Pro C18 Hydrosphere C18 Pro C18 RS

Three kinds of ODS, each with different separation characteristics

Comparison of hydrophobicity and hydrogen-bonding capacity of various columns

High performance reversed-phase column

Flavonoid glycosides

Saikosaponins

Analytical columns

● Superior separation of basic compounds

Useful for separation of basic compounds

Basic peptides

Excellent lot-to-lot reproducibility

Two types of test reports

Analytical columns

antioxidants

Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)

Guard columns

● Utilizes highly pure silica gel base ● Two types of test reports are included with

YMC-Triart C18　－Integration of triple artistic technologies－

YMC-Triart C18　－Integration of triple artistic technologies－

Low back-pressure　－Free from pressure problem－

Standard ODS　－Best balance hydrophobicity－

Reduced column bleed　－Operate at high sensitivity－

Improvement of loadability　－Minimizing strong solvent/sample loading effects－