Programme PhD / Master course
Advanced Microscopy and Vital Imaging
2014
Monday, June 2, 2014
Time Item Location
09.00 – 09.15 Welcome and brief overview of the course Frans Ramaekers
Dept. Molecular Cell Biology, Maastricht University Medical Center
UNS 50 F.5.111A 09.15 – 10.00 Basics of Microscopy
Marc van Zandvoort
Dept. Molecular Cell Biology, Maastricht University Medical Center
UNS 50 F.5.111A 10.15 – 12.45 Practical course: Bright field microscopy, a hands-on course
GROUP I
Jos Broers/Helma Kuijpers [Marc van Zandvoort] Maastricht University Medical Center
UNS 40 C.4.577
10.15 – 11.15 Fluorescence microscopy
GROUP II
Marc van Zandvoort
Dept. Molecular Cell Biology, Maastricht University Medical Center
UNS 50 F.5.111A
13.30 – 14.30 Fluorescence microscopy
GROUP I
Marc van Zandvoort
Dept. Molecular Cell Biology, Maastricht University Medical Center
UNS 50 F.5.111A
13.30 – 16.00 Practical course: Bright field microscopy, a hands-on course
GROUP II
Jos Broers/Helma Kuijpers [Marc van Zandvoort] Maastricht University Medical Center
UNS 40 C.4.577
Tuesday, June 3, 2014
Time Item Location
09.00 – 10.00 Principles of confocal scanning laser microscopy Edwin Lamers
Carl Zeiss Benelux
UNS 50 F.5.111A 10.00 – 11.00 Live cell imaging using GFP-technology in combination with CSLM
Jos Broers
Dept. Molecular Cell Biology, Maastricht University Medical Center
UNS 50 F.5.111A 11.00 – 12.00 Sample preparation
GROUPS 5-8 Frans Ramaekers
Dept. Molecular Cell Biology, Maastricht University Medical Center
UNS 50 F.5.111A
14.00 – 15.00 Sample preparation GROUPS 1-4 Frans Ramaekers
Dept. Molecular Cell Biology, Maastricht University Medical Center
UNS 50 F.5.111A
11.00 – 14.00 Demonstrations (according to schedule, 45 min per group) GROUPS 1-4
Confocal scanning laser microscopy
Jos Broers / Helma Kuijpers
Dept. Molecular Cell Biology, Maastricht University Medical Center
UNS 50 G.5.223A 14.00 – 17.00 Demonstrations (according to schedule, 45 min per group)
GROUPS 5-8
Confocal scanning laser microscopy
Jos Broers / Helma Kuijpers
Dept. Molecular Cell Biology, Maastricht University Medical Center
UNS 50 G.5.223A
Wednesday, June 4, 2014
Time Item Location
09.00 – 09.45 Analysis of the neuromuscular junction – quantitative microscopy Mario Losen
Dept. of Basic Neurosciences, Maastricht University Medical Center
UNS 50 F.5.111A 09.45 – 10.30 Backgrounds and applications of STED Microscopy
Patrick van Wieringen Leica Microsystems BV
UNS 50 F.5.111A
10.30 – 10.45 Coffee/tea break UNS 50 F.5.111A
10.45 – 11.30 Two-photon laser microscopy for the three-dimensional study of
tissues
Marc van Zandvoort
Dept. Molecular Cell Biology, Maastricht University Medical Center
UNS 50 F.5.111A
11.45 – 13.15 ImageJ GROUPS 5-7 Jos Broers
Dept. Molecular Cell Biology, Maastricht University Medical Center
UNS 50 F.5.111A 0f Pc ruimte 15.00 – 16.30 ImageJ GROUPS 1-4 Jos Broers
Dept. Molecular Cell Biology, Maastricht University Medical Center
UNS 50 F.5.111A Of pc ruimte 11.45 – 17.00 Demonstrations (according to schedule, 45 min per group)
GROUPS 1-7
Two-photon scanning laser microscopy
Timo Rademakers [Zhuojun Wu] Sanquin, Amsterdam
UNS 50 H.3.347
Thursday, June 5, 2014
Time Item Location
09.00 – 10.00 Advances in multiphoton microscopy – in vivo imaging in
cardiovascular research
Timo Rademakers Sanquin, Amsterdam
UNS 50 F.5.111A
10.00 – 11.00 High throughput and high content cellular analysis for systems
biology Winnok De Vos Ghent University UNS 50 F.5.111A 11.00 – 11.15 Coffee/tea break
11.15 – 12.15 Advanced Electron Microscopy Peter Peters
EM-Unit and Dept. Molecular Cell Biology, Maastricht University Medical Center
UNS 50 F.5.111A
12.30 Departure to FEI Nanoport Company, Eindhoven
Peter Peters /Helma Kuijpers
EM-Unit and Dept. Molecular Cell Biology, Maastricht University Medical Center
UNS 50 (by bus)
14.15 – 15.00 Welcome and introduction biological application of electron
microscopy
Wim Busing
Sr Applications Specialist NanoBiology, FEI Company, Eindhoven 15.00 – 16.30 Demo's on Quanta 3D FEG (SEM), Quanta 250 (SEM), and Tecnai
20 (TEM)
Wim Busing
Sr Applications Specialist Nano Biology,FEI Company, Eindhoven
16.45 Departure back to Maastricht University Medical Center FEI, Eindhoven
Maastricht Microscopy Meeting (M3) | Friday, June 6, 2014
Symposium Advanced Microscopy and Vital Imaging
Greepzaal, level 4, azM | Maastricht University Medical Center, P. Debyelaan 25, Maastricht
09.30 – 10.00 Arrival & Coffee
10.00 – 10.10 Welcome by Marc van Zandvoort
Dept. of Molecular Cell Biology, Maastricht University Medical Center
10.10 – 10.55 Speaker 1 Dept. of xxxx Title 10.55 – 11.40 Speaker 2 Dept. of xxxxxx Title 11.40 – 12.30 Speaker 3 Dept. of xxxxxx Title 12.30 – 13.45 Lunch 13.45 – 14.30 Speaker 4 Dept. ofxxxxxx Title 14.30 – 15.15 Speaker 5 Dept. of xxxxx Title 15.15 – 16.00 Speaker 6 Dept. of xxxx Title 16.00 - 16.30 Speaker 7 Dept. of xxxx Title
16.30 – 17.30 Closure & Drinks
Participation is free, but please note that registration is obligatory. You can register by sending an e-mail, including your name and department, to the secretary of Molecular Cell Biology, Maastricht University Medical Center.