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EGF receptor lysosomal degradation is delayed in the cells stimulated with EGF-Quantum dot bioconjugate but earlier key

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Figure 1: Time-dependent imaging of EGFR and EGF-QDs distribution in HeLa cells.  HeLa cells were incubated with  (A) 2 nM EGF or (B) bEGF-savQD (prepared by incubation of 2 nM bEGF with 0.5 nM QDs) using pulse-chase protocol for the indicated  time
Figure 2: Immuno-co-localization of EGFR and bEGF-savQD with EEA1.  (A) HeLa cells were incubated with 2 nM EGF  or bEGF-savQD (2:0.5 nM) using pulse-chase protocol for the indicated time
Figure 3: Analysis of endosome fusions in HeLa cells.  (A) bEGF-savQDs with different emitting wavelengths 525 and 655 nm  were added sequentially
Figure 4: Effect of wortmannin on EGFR endocytosis stimulated by EGF or bEGF-savQDs.  Control or wortmannin-treated  (100 nM) HeLa cells were incubated with (A) 2 nM EGF or (B) bEGF-savQD (2:0.5 nM) using a pulse-chase protocol for 60 min
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