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Co-transplantation of mesenchymal stem cells improves spermatogonial stem cell transplantation efficiency in mice

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Academic year: 2020

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Figure

Fig. 1 Optimization of the mouse model for infertility. Several doses of CdCl2(0.5, 1.0, 2.0, and 3.0 mg/kg body weight) were tested incombination with busulfan (40 mg/kg body weight)
Fig. 2 Mesenchymal stem cell culture. RFPwere obtained by incubating antibody array membranes with conditioned media from TGFß1-treated and non-treated MSC cultures (at 3 weeks).A+ MSCs were cultured in the absence (A, A′) or presence of TGFß1 (B, B′) for
Fig. 3 Transplantations. Transplantations were performed in GFP− busulfan- and CdCl2-treated mice using SSCs from 5- to 7-day-old GFP+ miceand/or RFP+ MSCs
Fig. 4 Testicular histology 3 months after transplantation. The significant increase of testis size (a) and testis-to-body weight ratio (b) was foundin transplanted groups compared to controls (control vs
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