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A Practical Guide to Rodent Islet Isolation and Assessment

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Figure

Fig. 1. Islet morphology following isolation. a18shown among pancreatic acinar tissue on the day of isolation.purified from acinar tissue, after incubation at 37°C and 5% CO A freshly isolated islet b Islets2 for–20 h
Fig. 2. Assessment of islet viability by fluorescence intensity of propidium iodide. acence (, b Untreated(healthy) islets shown in brightfield (a, top panel) display only trace amounts of PI fluorescence (a, bot-tom panel)
Fig. 3. A comparison of glucose-stimulated calcium (GSCa) response forislets cultured overnight in 5.5 mM glucose (5 G) and 11 mM glucose(11 G)
Fig. 4. Anatomy of the mouse upper intraperitoneal (IP) cavity. NOTE:The top is the caudal portion of the mouse, bottom is the rostral portion,ie, this is from the perspective of the mouse lying on its back tail awayfrom the surgeon and nose toward the surgeon.
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