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Zinc Finger Nucleases as tools to understand and treat human diseases

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Figure

Figure 1 Strategies for generation of designer zinc-finger proteinsof characterized zinc fingers for each DNA triplet, followed by random PCR assembly and selection of the best assembled zinc finger trimer
Figure 2 Functional domains attached to zinc finger proteinswith PC4 (2PHE), the Kruppel associated box domain (1V65), the histone H3K9 methyl transferase Suv39H1 (2R3A), HhaI DNA methyl transferase
Figure 3 Site specific cutting requires heterodimerization of two independently designed ZFNs

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