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Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families

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Academic year: 2020

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Figure

Figure 1 Outline of the procedures used for isolation of nuclei. A) Species-specific adjustments at specific steps of isolation of nuclei fromthe leaves of tobacco, potato and apple
Figure 2 Analysis of protein fractions from different steps ofnuclei isolation. The protein fractions obtained following the mainsteps of the procedure used for isolation of nuclei from leaves ofapple are shown
Figure 3 Preparations of nuclei obtained following the finalpurification on 35% Percoll cushion by centrifugation.Preparations of nuclei from leaves of (A) tobacco, (B) potato and (C)apple obtained after the final purification on 35% Percoll cushion bycent

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