Product and Pricelist

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Product and Pricelist

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Introduction

This catalogue contains test kits and components for the rapid detection of biologial agents such as toxins, micro organisms and viruses. The testkits are based on the ABICAP technology (ABICAP = Antibody Binding Immune Column for Analytical Purpose). ABICAP is a flow through immuno assay system for rapid and ultra sensitive detection. It is based on the 3D-immunofiltration1_{and PolyHRP-technology}2_{and allows}

to combination of analyte extraction and direct quantitative read out on the test columns.

The system is well suited for decentralised routine testing. To this end ready to use test kits, equipment (workstation in 96 –hole format) and a handheld photometer are offered.

In addition, for research studies and customer specific development, assay components and reagents are available and development services are offered.

1 see booklet: "introduction to 3D Immunofiltration technology" 2 see booklet: "introduction to polyHRP technology"

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Index

ABICAP

®

_{classic Test Kits}

Microorganisms... Viruses... Toxins...

ABICAP

®

_{multilayer Test Kits}

Microorganisms... Viruses... Toxins...

Auxiliary reagents and components for ABICAP

®

_{Test Kits}

Positive controls lyophilized pellets... Special buffers and blocker... Sample preparation tools...

Equipment

ABICAP®_{Photometer...} Workstations...

Antibodies

Microorganisms... Viruses... Toxins ...

Antigens

Microbiological agents... Toxins...

Assay components and tools

Microcolumns... Buffer... PolyHRP Substrate... PolyHRP Reagents... Specific HRP- and PolyHRP Conjugates...

Services

ABICAP®_{custom development...} PolyHRP custom conjugation...

2 2 2 3 3 3 4 5 5 6 6 7 8 8 9 9 10 10 11 11 11 12 12

Index

From a order value of 5000,00 € or higher, shipping costs are included.

Terms and conditions could be found on our website www.diavita.de.

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negative

weak positive

strong positive

101 00 002 CAP Francisella tularensis ABICAP®_Classic _480,00

101 00 003 CAP Yersinia pestis F1 ABICAP®_Classic _480,00

101 00 006 CAP B. anthracis spores ABICAP®_Classic _480,00

101 00 007 CAP B. anthracis protective antigen ABICAP®_Classic _480,00

Microorganisms

Viruses

101 00 005 CAP Ebola virus ABICAP®_Classic _480,00

101 00 011 CAP Flaviviruses ABICAP®_Classic _480,00

101 00 012 CAP Venezuela Equine Encephalitis (VEE) ABICAP®_Classic _{(under development)}

101 00 019 CAP Eastern Equine Encephalitis (EEE) ABICAP®_Classic _{(under development)}

Toxins

101 00 008 CAP Botulinus toxin A ABICAP®_Classic _{upon request}

101 00 013 CAP Botulinus toxin B ABICAP®_Classic _480,00

101 00 016 CAP Botulinus toxin E ABICAP®_Classic _480,00

101 00 017 CAP Staphylococcus Enterotoxin B (SEB) ABICAP®_Classic _480,00

101 00 018 CAP Ricin ABICAP®_Classic _480,00

Productnumber Product Price in €

2 ABICAP

®

_{Test Kits}

ABICAP

®

classic Test Kits

The ABICAP classic Test kit contains 24 ABICAP columns with one specific detection filter and all reagents and instructions needed to perform quantitative assays.

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3 ABICAP

®

multilayer Test Kits

The ABICAP multilayer test kit contains 24 ABICAP columns in a 3 filter arrangement and all reagents to perform quantitative tests.

negative

weak positive

strong positive

101 00 002 CAP Francisella tularensis ABICAP®_multilayer _720,00

101 00 003 CAP Yersinia pestis ABICAP®_multilayer _720,00

Microorganisms

Toxins

101 00 008 CAP Botulinus toxin A ABICAP®_multilayer _720,00

101 00 013 CAP Botulinus toxin B ABICAP®_multilayer _720,00

101 00 016 CAP Botulinus toxin E ABICAP®_multilayer _720,00

101 00 017 CAP Staphylococcus Enterotoxin B (SEB) ABICAP®_multilayer _720,00

101 00 018 CAP Ricin ABICAP®_multilayer _720,00

Negative control Filter (indicates non specific binding) Specific Measurement filter Positive control Filter (indicates function of assay reagents

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Auxiliary reagents and components for ABICAP

®

Test Kits

104 00 008 PC Botulinus- toxin A (toxoid) 2 controls 12,00

104 00 013 PC Botulinus- toxoid B (toxoid) 2 controls 12,00

104 00 016 PC Botulinus- toxoid E (toxoid) 2 controls 12,00

101 00 017 PC Staphylococcus Enterotoxin B (SEB) 2 controls 12,00

104 00 018 PC Ricin 2 controls 14,00

4 Auxiliary reagents and components for ABICAP

®

_{Test Kits}

Microorganisms

104 00 019 PC Legionella pneumophila serotyp 1 2 controls (under development)

104 00 002 PC Francisella tularensis 2 controls 12,00

104 00 003 PC F1 control (Yersinia pestis) 2 controls 12,00

104 00 006 PC B. anthracis spores 2 controls 14,00

104 00 007 PC B. anthracis protective antigen 2 controls 14,00

Viruses

104 00 005 PC Ebola control 2 controls 12,00

104 00 011 PC Flaviviruses 2 controls (under development)

104 00 012 PC Venezuela Equine Encephalitis (VEE) 2 controls (under development)

104 00 019 PC Eastern Equine Encephalitis (EEE) 2 controls (under development)

Toxins

Positive controls are lyophilised reagent pellets. They are reconstituted in destilled water.

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302 00 005 CMP sorbent filter (fig. 1) 5 20,00

302 00 009 CMP 10-20µm PE ABICAP®_prefilter ₂₄ _24,00

302 00 013 CMP filter holder for prefilter (fig. 2) 10 60,00

302 00 007 CMP sample application funnel, 10 ml (fig. 3) 6 15,00

302 00 008 CMP sample application funnel, 50 ml (fig. 4) 12 39,00

5 Sample preparation tools

Productnumber Product Quantity Price in €

Special buffers and blocker

201 04 005 BUF HAMA - Blocker*

(Human anti-mouse antibody) 20-90 mg10 mg

100 mg

25,00 20,00/10mg

175,00 104 00 002 BUF LPS Extraction buffer**

(Lipopolysaccharid) 100 ml50 ml 15,00 25,00

Auxiliary reagents and components for ABICAP

®

_{Test Kits}

For the treatment of complex sample material and prefiltration we offer sample preparation tools. They have to be selected by the customers in dependence on the sample matrix.

For large volume application sample funnels (figure 3,4) are offered.

*Human anti-mouse antibodies might course false positive signals. HAMA-Blocker reduces the frequency of false results by blocking a wide range of HAM antibodies regardless of the anti-mouse epitope

**Lipopolysaccharid is a useful buffer to extract LPS from gram-negative cells; thougt for sample preparations in the special case of the Francisella tularensis assay

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2

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6

302 00 001 CMP ABICAP®_{Rack for 96 ABICAP}®_Microcolumns consisting of one 96-hole frame, a stackable frame and one waste basket (fig. 6)

1 39,00

302 00 002 CMP Stackable frame for ABICAP®_{Rack, plexiglas} ₁ _20,00

302 00 003 CMP Stackable frame for ABICAP®_Rack,

paperboard, disposable (fig. 7) 25 5,00

302 00 004 CMP Waste basket for ABICAP®_{Rack, sterilizable}

(fig. 8) 1 20,00

302 00 005 CMP 96-hole frame to hold ABICAP®_Microcolumns ₁ _4,00

Workstations (not included in the Test Kits)

301 00 001 RDR ABICAP®_{Photometer (portable) for ABICAP}® Classic and ABICAP®_{Multilayer with key pad,} incl. chip card reader, power supply, smart card reader, RS232 interface, weight approx. 300g, dimension 18x 9x3cm (fig. 5)

1 840,00

Accessories:

Equipment

ABICAP

®

_{Photometer (not included in the Test Kits)}

301 00 002 RDR PC software and cable 1 179,00

Equipment

5 6

7 8

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Productnumber Product Amount Price in €/mg 210 06 003 MAB Monoclonal anti-Bacillus anthracis

Protective Antigen

clon 249 (for coating) IgG1 1 to 9 mg 10 to 99 mg 100 to 500 mg >500 mg 240,00 180,00 120,00 upon request 210 06 004 MAB Monoclonal anti-Bacillus anthracis

Protec-tive Antigen

clon 352 (for conjugation) IgG1 1 to 9 mg 10 to 99 mg 100 to 500 mg >500 mg 240,00 180,00 120,00 upon request 210 06 061 MAB Monoclonal anti-Bacillus anthracis

Protec-tive Antigen, biotinylated

clon 352 IgG1

1 to 9 mg

>9 mg upon request480,00 210 06 005 MAB Monoclonal anti-Bacillus anthracis spores,

clon 38 (for coating) IgG2a 1 to 9 mg 10 to 99 mg 100 to 249 mg > 250 mg 360,00 280,00 210,00 upon request 210 06 006 MAB Monoclonal anti-Bacillus anthracis spores,

clon 37 (for conjugation) IgG2a 1 to 9 mg 10 to 99 mg 100 to 249 mg > 250 mg 360,00 280,00 210,00 upon request 210 06 062 MAB Monoclonal anti-Bacillus anthracis spores,

clon 37, biotinylated IgG2a

1 to 9 mg

>9 mg upon request620,00 210 06 022 MAB Monoclonal anti-Francisella tularensis,

Reacts with LPS of F. tularensis IgG3 1 to 9 mg 10 to 99 mg 100 to 500 mg >500 mg 240,00 180,00 120,00 upon request 210 06 063 MAB Monoclonal anti-Francisella tularensis,

biotinylated

Reacts with LPS of F. tularensis IgG3

1 to 9 mg

>9 mg upon request420,00 210 06 056 PAB Polyclonal anti-Legionella pneumophilia,

Serotyp 1, G118 1 to 9 mg>9 mg upon request310,00

210 06 063 MAB Monoclonal anti-Legionella pneumophilia,

Serotyp 1, 6G5 10 to 99 mg1 to 9 mg 100 to 500 mg >500 mg 240,00 180,00 120,00 upon request 210 06 039 MAB Monoclonal anti-Legionella pneumophila,

Serotyp 1

Reacts with LPS membrane of Legionella pneumophila, IgG1 1 to 9 mg 10 to 99 mg 100 to 500 mg >500 mg 240,00 180,00 120,00 upon request

210 06 048 MAB Monoclonal anti-Staphylococcus aureus 1 to 9 mg 240,00

Antibodies

Microorganisms

7 Antibodies

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210 06 053 MAB Monoclonal anti-Ebola Virus, (for coating) IgG 1 to 9 mg 10 to 99 mg 100 to 500 mg >500 mg 360,00 280,00 210,00 upon request 210 06 059 MAB Monoclonal anti-Ebola Virus,

(for conjugation) IgG 1 to 9 mg 10 to 99 mg 100 to 500 mg >500 mg 360,00 280,00 210,00 upon request 210 06 061 MAB Monoclonal anti-Ebola Virus,

IgG , biotinylated 1 to 9 mg>9 mg upon request620,00

210 06 051 PAB Polyclonal anti-Venezuela Equine

Encephalitis

affinity purified mouse IgG 1 to 9 mg 10 to 99 mg 100 to 500 mg >500 mg 240,00 180,00 120,00 upon request 210 06 055 PAB Polyclonal anti-Eastern Equine Encephalitis

affinity purified mouse IgG 1 to 9 mg 10 to 99 mg 100 to 500 mg >500 mg 240,00 180,00 120,00 upon request 210 06 056 PAB Polyclonal anti-Western Equine

Encephalitis

affinity purified mouse IgG 1 to 9 mg 10 to 99 mg 100 to 500 mg >500 mg 240,00 180,00 120,00 upon request 210 06 060 MAB Monoclonal anti-Flavivirus, group specific

IgG2a 10 to 99 mg1 to 9 mg 100 to 500 mg >500 mg 240,00 180,00 120,00 upon request 210 06 066 MAB Monoclonal anti-Flavivirus, group specific

IgG2a, biotinylated 10 to 99 mg1 to 9 mg 100 to 500 mg >500 mg 360,00 280,00 210,00 upon request

Viruses

Productnumber Product Amount Price in €/mg

8 Antibodies

210 06 049 MAB Monoclonal anti-Staphylococcus aureus

Enterotoxin B,

Specific for enterotoxins, B, no crossreactivity with C, D , E , clon 754 (for conjugation), IgG1

1 to 9 mg 10 to 99 mg 100 to 500 mg >500 mg 240,00 180,00 120,00 upon request 210 06 064 MAB Monoclonal anti-Staphylococcus aureus

Enterotoxin B, biotinylated

Specific for enterotoxins, B, no crossreactivity with C, D , E , clon 754 (for conjugation), IgG1

1 to 9 mg

>9 mg upon request420,00 210 06 051 MAB Monoclonal anti-Yersinia pestis-F1 Antigen,

Reacts with capsule bearing strains of Y. pestis, IgG1 1 to 9 mg 10 to 99 mg 100 to 500 mg >500 mg 240,00 180,00 120,00 upon request 210 06 065 MAB Monoclonal anti-Yersinia pestis-F1 Antigen,

biotinylated

Reacts with capsule bearing strains of Y. pestis, IgG1

1 to 9 mg

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9 Antibodies

210 06 054 MAB Monoclonal anti-Botulinus A

clon 22G8, (for coating) IgG2a/IgM 100µg 1 to 9 mg 10 to 99 mg 100 to 500 mg >500 mg 100,00 520,00 440,00 360,00 upon request

210 06 055 MAB Monoclonal anti-Botulinus B

clon C3/4 (for coating) IgG1 100µg 1 to 9 mg 10 to 99 mg 100 to 500 mg >500 mg 100,00 520,00 440,00 360,00 upon request

210 06 056 MAB Monoclonal anti-Botulinus E

clon 21D4 (for coating) IgG1 100µg 1 to 9 mg 10 to 99 mg 100 to 500 mg >500 mg 100,00 520,00 440,00 360,00 upon request 210 06 052 PAB Polyclonal anti-Botulinus A

horse, F(ab)2 fragments

1 to 9 mg 10 to 99 mg 100 to 500 mg >500 mg 160,00 120,00 90,00 upon request 210 06 058 PAB Polyclonal anti-Botulinus A, biotinylated

1 to 9 mg

>9 mg upon request310,00 210 06 053 PAB Polyclonal anti-Botulinus B

1 to 9 mg 10 to 99 mg 100 to 500 mg >500 mg 160,00 120,00 90,00 upon request 210 06 059 PAB Polyclonal anti-Botulinus B, biotinylated

1 to 9 mg

>9 mg upon request310,00 210 06 054 PAB Polyclonal Botulinus E

1 to 9 mg 10 to 99 mg 100 to 500 mg >500 mg 160,00 120,00 90,00 upon request 210 06 060 PAB Polyclonal Botulinus E, biotinylated

1 to 9 mg

>9 mg upon request288,00

210 06 061 PAB Polyconal anti-Botulinus E rabbit IgG fraction 100µg

1 to 9 mg 10 to 99 mg 100 to 500 mg >500 mg 50,00 240,00 180,00 120,00 upon request

Toxins

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10 Antigens

210 07 005 AGN Staphylococcus aureus Enterotoxin B,

purified 100 µg1 mg 240,0065,00

210 07 006 AGN Ricin, Ammoniumsulphate precipitated 100 µg

1 mg 480,0085,00

Toxins

Productnumber Product Amount Price in €

210 07 001 AGN Bacillus anthracis Protective Antigen (PA),

isolated from the vaccine strain STI-1 100 µg1 mg 480,0085,00

210 07 053 AGN Bacillus anthracis spores

attenuated, strain, inactivated 10

6_spores

109_spores 150,00 _780,00

210 07 002 AGN Y.pestis F1 Antigen 100 µg

1 mg 480,0085,00

210 07 003 AGN F. Tularensis, LPS Antigen 100 µg

1 mg 480,0085,00

210 07 004 AGN Legionella pneumophila serogroup 1 LPS upon request

Antigens

Microbiological Antigens

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11 Assay components and tools

Assay components and tools

102 00 003 CMP Microcolumns with frits for protein coating in

packages of 192 columns 100-1000 packs1-10 packs

>1000 packs

0,62/column 0,52/column upon request

201 04 001 CAS Casein Diluent / Blocker, ready-to-use

(UCDB) 1.000 ml500 ml 2.000 ml 4.000 ml 5.000 ml 10.000 ml 20.000 ml 40.000 ml 50.000 ml 150,00 270,00 500,00 800,00 1.000,00 1.600,00 2.800,00 5.000,00 6.000,00

201 04 002 BUF Blocking buffer 500 ml

1000 ml 2000 ml > 2000 ml 150,00 270,00 500,00 upon request

201 04 003 BUF PolyHRP Conjugate Stabilizer, ready-to-use 500 ml

1.000 ml 2.000 ml 4.000 ml 5.000 ml 10.000 ml 20.000 ml 40.000 ml 50.000 ml 180,00 330,00 600,00 1.100,00 1.200,00 2.200,00 3.500,00 6.000,00 7.000,00

201 04 004 BUF Substrate buffer 500 ml

1.000 ml 2.000 ml 4.000 ml 5.000 ml 10.000 ml 20.000 ml 40.000 ml 50.000 ml 20,00 30,00 50,00 90,00 100,00 150,00 250,00 300,00 380,00

Buffer

Many reagents used in conventional immunoassay can be applied to the 3D-Immunofiltration formate. The coating procedure of the porous filters is simple and allows the manufacturing of your own individual column assays.

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Assay components and tools

12 PolyHRP Reagents

201 00 002 CJG SA-PolyHRP 40 1 mg 2 to 9 mg 10 to 99 mg 100 to 599 mg > 600 340,00 310,00 280,00 260,00 240,00

Productnumber Product Amount Price in €/mg

PolyHRP is a supermolecular enzyme complex with up to 400 molecules. The PolyHRP complex can be conjugated to various ligands and receptors. These PolyHRP-conjugates can be utilized in ELISA assays, immunoblots, immunofiltration, DNA/RNA Hybridisation. Its specific activity is by magnitudes superior compared to conventional conjugates, allowing for an substantial inhancement of assay sensitivity, optimization of assay time and reduction of reagent consumption

.

201 03 001 CJG Monoclonal anti-Y. Pestis-F1 - PolyHRP 40 100 µg

0,5 mg 1 mg > 1 mg 300,00 1200,00 1900,00 upon request

201 03 002 CJG Monoclonal anti-F. Tularensis - PolyHRP 40 100 µg

201 03 003 CJG Monoclonal anti-Ebola Virus - PolyHRP 40 100 µg

0,5 mg 1 mg

380,00 1500,00 2400,00

201 03 004 CJG Monoclonal anti-B.anthracis spores HRP 100 µg

Specific HRP- and PolyHRP Conjugates

PolyHRP Substrate

201 03 001 SUB sTMB substrate solution, soluble, for

ELISA-applications; ready-to-use 100 ml250 ml 500 ml 1000 ml 30,00 80,00 135,00 235,00/L 201 03 002 SUB pTMB substrate solution, precipitating, for

3D-IF rapid test applications (ABICAP®_); ready-to-use 50 ml 100 ml 250 ml 500 ml 1000 ml 90,00 110,00 190,00 290,00 450,00

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Services

ABICAP

®

_{custom development}

PolyHRP custom conjugation

102 01 001 SEV 3D-IF rapid test study feasibility 4.900

102 01 002 SEV 3D-IF optimisation 4.200 - 12.000

102 01 003 SEV 3D-IF validation 3.000 - 12.000

Productnumber Product Quantity/Amount Price in €

201 04 001 SEV PolyHRP ELISA study (feasibility) 1 4.100

201 04 002 SEV PolyHRP - pilot conjugation up to 10 mg 4.800

201 04 003 SEV PolyHRP - conjugation

This conjugation is based on the PolyHRP - pilot conjugation (201 04 002 SEV) and can only be ordered additionally

11 - 50 mg 51 - 100 mg > 100 mg 340,00 / mg 310,00 / mg 180,00 / mg 3D-Immunofiltration assays and the PolyHRP Technology are useful in many areas of the diagnostic and biotech industry to increase sensitivity and to reduce the assay time. Our research partner SENOVA is prepared to assist our customers in specific product development by offering:

• Feasibility studies • Reagent development

• Assay development and optimisation • Custom conjugation

• Design and development • Validation and

• Production