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Selective plane illumination microscopy techniques in developmental biology

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Figure

Fig. 1. The concept behind fluorescence light-sheet microscopy. Inlight-sheet microscopy, fluorescence excitation (blue arrow) anddetection (green arrow) are split into two distinct optical paths
Table 1. Overview of fluorescence light-sheet techniques in comparison with epifluorescence, confocal and two-photonmicroscopy
Fig. 2. Advantages of light-sheet microscopy compared with confocal microscopy. Light-sheet microscopy features faster acquisition andmicroscopy (sample (A), thereby exposing the sample to high-intensity light not only in the plane of interest, but also ab
Fig. 3. Typical SPIM components (not to scale). A typical SPIM setup consists of an illumination, a detection and (optionally) a photo-manipulationunit
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