www.wjpr.net Vol 6, Issue 16, 2017. 1111
ANTI-INFLAMMATORY ACTIVITY OF
IPOMOEA TURPETHUM
LINN.
ON CARRAGEENAN INDUCED PAW EDEMA IN WISTAR
RATS
Dr. Vrunda V. Shah1*,Dr. Vipul K. Shah2, Dr. Navin R. Sheth3 and Dr. Madhabhai M.
Patel4
1,4
Department of Pharmacognosy, Shree Swaminarayan Sanskar Pharmacy College, Zundal,
Gandhinagar-382421, Gujarat, India.
2
Troikaa Pharmaceuticals Ltd., Ahmedabad, Gujarat, India.
3
Vice Chancellor of Gujarat Technological University, Ahmedabad, Gujarat, India.
ABSTRACT
The hydro alcoholic extract of Ipomoea turpethum root was
investigated for evaluation of anti-inflammatory activity in rats at a
dose 200 and 400 mg/kg in carrageenan-induced paw edema method.
Hydro alcoholic extract of Ipomoea turpethum root significantly
reduced the migration of neutrophils and monocytes. Results of in vivo
activity lead to the conclusion that the hydro alcoholic extract of
Ipomoea turpethum root showed predominantly significant activity in
dose dependent manner, which is comparable to the standard drug
Aspirin.
KEYWORDS: Anti-inflammatory, hydro alcoholic extract, Ipomoea
turpethum.
INTRODUCTION
Inflammation is considered as a primary physiologic defense mechanism that helps body to
protect itself against infection, burn, toxic chemicals, allergens or other noxious stimuli, an
uncontrolled and persistent inflammation may act as an etiologic factor for many of these
chronic illnesses.[1] The side effects of the currently available anti-inflammatory drugs pose a
major problem during their clinical uses.[2] Therefore, the development of newer and more
potent anti-inflammatory drugs with lesser side effects is necessary.
Volume 6, Issue 16, 1111-1115. Research Article ISSN 2277–7105
Article Received on 14 October 2017,
Revised on 03 Nov. 2017, Accepted on 24 Nov. 2017 DOI: 10.20959/wjpr201716-10283
*Corresponding Author
Dr. Vrunda V. Shah
Department of
Pharmacognosy, Shree
Swaminarayan Sanskar
Pharmacy College, Zundal,
Gandhinagar-382421,
www.wjpr.net Vol 6, Issue 16, 2017. 1112
The Ipomoea turpethum syn. Operculina turpethum which is commonly known as trivit, The
Root is bitter, sweet, with a sharp taste; heating, anthelmintic, purgative, anti pyretic,
alexiteric; useful in ascites, leucoderma, itch, ulcers, constipation, abdominal troubles,
inflammation, anaemia, fevers, biliousness, piles, erysipelas, tumors, jaundice, ophthalmia,
bites from insects; The black variety is a powerful drastic; useful in loss of consciousness,
burning sensation, intoxication.The white variety is a moderately mild cathartic; useful in
bilious fevers, inflammation and diseases of the abdomen.[3]
MATERIAL AND METHOD
Plant material
The Roots of Ipomoea turpethum were collected from Saurashtra region, Junagadh during the
month of October-November. The plant was authentified by Botanical Survey of India,
Jodhpur and a voucher specimen no. SU/DPS/Herb/09 was deposited. Further, the plant was
identified by comparing it morphologically and microscopically with the description given in
different standard texts and floras.[3,4] Fresh Roots of plant were cleaned, dried at room
temperature and powdered.
Preparation of Extract
The shade dried roots were subjected to physical evaluation. The standardized coarse powder
of the root was subjected to macerate with hydroalcohol (50%) for sufficient period of time,
then filter it and solvent was completely removed under reduced pressure using rotary flash
evaporator. The extracts were suspended in 7% acacia solution for pharmacological study.
Acute toxicity study
Different groups of five mice each were given graded doses of the plant extract (0.5-3.0 g/kg,
orally) and were kept under constant observation for 6 h to note any behavioral changes and
mortality was recorded after 24 h of the drug administration.[5] The experiment was carried
out according to Committee for the Purpose of Control and Supervision of Experiments on
Animals (CPCSEA) guidelines and Institutional Animal Ethical Committee approved all the
procedures.
Protocol for Carrageenan induced hind paw edema
Evaluation of anti-inflammatory activity Carrageenan-induced rat paw edema: Rats were
divided into four groups of six animals each. The first group served as the control and
www.wjpr.net Vol 6, Issue 16, 2017. 1113
with ITHAE (100mg/kg, 200 mg/kg respectively) and the fourth group was administered with
standard drug, Aspirin (200 mg/kg, orally). A mark was made on both the hind paws just
below the tibiotarsal junction so that every time the paw could be dipped in the mercury
column of plethysmometer up to the mark to ensure constant paw volume.[6]
Forty-five minutes after treatment, an inflammatory edema was induced in the left hind paw
by injection of 0.1 ml of Carrageenan (1% w/v) in the plantar tissue of all the animals. The
paw volume was measured in control, standard and sample treated groups immediately before
and after 3 h of Carrageenan injection.[7, 8, 9, 10] The degree of edema formation was assayed
by the percentage increase in paw treated with standard drug and those treated with extract.
These were compared with the increased paw volume of control animals. Thus, percentage
inhibition of paw volume in treated animals i. e. edema rate (E) % = (Vt/Vc) x100,
Which was used for calculating the percentage inhibition rate % = 1-(Vt/Vc) x100, where Vt
and Vc are the mean relative changes in the paw volume of the test and control
respectively.[10]
Group I: Vehicle (7% gum acacia in distilled water, 2ml/kg. p.o.) +
0.1 ml Carrageenan (1% w/v, s.c.)
Group II: ITHAE (100 mg/kg, p.o.) + 0.1 ml Carrageenan (1% w/v, s.c.)
Group III: ITHAE (200 mg/kg, p.o.) + 0.1 ml Carrageenan (1% w/v, s.c.)
Group IV: Aspirin (200 mg/kg, p.o.) + 0.1 ml Carrageenan (1% w/v, s.c.)
Statistical analysis
For determinations of significant inter group difference, mean paw edema ± S.E.M. were
analyzed by One way analysis of variance (ANNOVA) using Sigma Stat Software.[11]
RESULT AND DISCUSSION
The preliminary Anti-inflammatory activity was evaluated by rat-paw edema model which
www.wjpr.net Vol 6, Issue 16, 2017. 1114 Table I: Preliminary Anti-inflammatory activity by rat-paw edema model.
Treatment Dose Paw edema (ml)
after 3 hr
% Inhibition Control
(Carrageenan)
Vehicle
(2ml/kg) 0.504±0.0807 -
Standard
(Aspirin) 200mg/kg 0.062±0.0203 87.698 %
ITHAE 100mg/kg 0.290±0.0766 42.460 %
ITHAE 200mg/kg 0.232±0.0128 53.968 %
Value are mean ±SEM; n=5. Compared to Control (P<0.001)
Results show (table I) that % Inhibition of paw edema is increased in dose dependent manner
in groups treated with Ipomoea turpethum Hydroalcoholic (50%) extract as compared to
control.
CONCLUSION
From present study, results show that the roots of Ipomoea turpethum has a strong
Anti-inflammatory activity. Hydro alcoholic extract of Ipomoea turpethum root significantly
reduced the migration of neutrophils and monocytes. The underlying mechanism needs to be
studied in future work.
REFERENCES
1. Kumar V, Abbas AK, Fausto N, (eds.) In: Robbins and Cotran pathologic basis of
disease. 7th ed. Philadelphia, Elsevier Saunders, 2004; 47-86.
2. Mattison N, Trimple AG, Lasagna I, New drug development in the United States, 1963
through 1984. Clin Pharmacol Ther, 1998; 290-301.
3. Kirtikar KR, Basu BD, Indian medicinal plants, 2nd Ed., Vol-III: 1991; 1730.
4. The Ayurvedic pharmacopoeia of India, Government of India- Ministry of Health And
Family Welfare; Part-I, Vol-III, 214-215.
5. Khalid HJ, Anwar HG, Evaluation of the protective potential of Artemisia maritime
extract on acetaminophen and CCl4 induced liver damage. Journal of
Ethnopharmacology, 1995; 47: 43-47.
6. Vishnukanta, Rana AC, Analgesic and Anti-Inflammatory Activity of Hydroalcoholic
Extract of Leaves of Plumbago zeylanica. Phcog Mag, 2008; 4.
7. Vendruscolo A, Takaki I, Amado LEB, Dantas JA, Amado CAB, Suman RKN,
Anti-inflammatory and antinociceptive activities of Zingiber officinale Roscoe essential oil in
www.wjpr.net Vol 6, Issue 16, 2017. 1115
8. Balian S, Ahmad S, Zafar R, Anti-inflammatory activity of leaf and leaf callus of Silybum
marianum (L.) Gaertn. In albino rats. Ind. J. Pharmacol, 2006; 38(3):213-214.
9. Sutradhar RK, Rahman AKMM, Ahmad MU, Datta BK, Bachar SC, Saha A,
Anti-inflammatory activity of Sida cordifolia Linn. Ind. J. Pharmacol, 2006; 38(3): 207-208.
10.Ahmet CO, Ufuk M, Hatice O, Nureddin C, Remzi E, Hanefi O, Anti-inflammatory and
Hepatoprotective Activities of Trigonella foenum-graecum L. Pharmacologyonline, 2008;
2: 126-132.
11.Ratheesh M, Helen A, Anti-inflammatory activity of Ruta graveolens Linn on
carrageenan induced paw edema in wistar male rats. African Journal of Biotechnology,
