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ALS2/Alsin regulates Rac PAK signaling and neurite outgrowth

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FIGURE 1.GST-PBD. The bound Rho, Rac, and Cdc42 were then detected by immunoblotting(detectedbyimmunoblottingsoastodemonstrateequaltransfectionefficiencies(GEF domain (in ALS2�DH) abrogates the stimulatory effect on Rac activity.of fold increases in GTPase
FIGURE 2. ALS2 stimulates PAK1 activity.Rac1 ( PAK1 in vitro kinase assays were performedwith CHO cells co-transfected with PAK1, Rac1, and ALS2, ALS2�DH, or vector pCIne-oCAT (CAT)
FIGURE 4. ALS2 is present in the growth coneand co-localizes with Rac. A, endogenous ALS2andF-actinin2DIVrathippocampalneurons;notethe presence of ALS2 in growth cones (arrows)
Fig. S2). However, ALS2 significantly increased (by 1.5-fold) the lengthof the longest neurite (Fig

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