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Case Report Intraductal papillary neoplasm of the bile duct, gastric type, arising in the intrapancreatic common bile duct could progress to colloid carcinoma: report of a case

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Int J Clin Exp Pathol 2015;8(5):5848-5855 www.ijcep.com /ISSN:1936-2625/IJCEP0007856

Case Report

Intraductal papillary neoplasm of the bile duct, gastric

type, arising in the intrapancreatic common bile duct

could progress to colloid carcinoma: report of a case

Shogo Tajima1,2, Akihiko Ohata3, Kenji Koda2, Yasuhiko Maruyama3

1Department of Pathology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan; Departments of 2Pathology, 3Gastroenterology, Fujieda Municipal General Hospital, Shizuoka, Japan

Received March 12, 2015; Accepted April 26, 2015; Epub May 1, 2015; Published May 15, 2015

Abstract: Intraductal papillary neoplasm of the bile duct (IPNB) exists in a pathway of multistep-carcinogenesis toward cholangiocarcinoma. Four subtypes are observed in IPNB, pancreatobiliary type, intestinal type, gastric type, and oncocytic type, similarly to the corresponding disease in the pancreas, intraductal papillary mucinous neoplasm (IPMN). IPNB can present with or without macroscopically visible mucin secretion. IPNB usually progresses to tubu-lar adenocarcinoma. However, there are a limited number of well-described cases of gastric-type IPNB progressing not to tubular adenocarcinoma but to colloid carcinoma. Herein, we present a case of an 82-year-old female patient with gastric-type IPNB in the intrapancreatic common bile duct without macroscopically visible mucin secretion, which progressed to colloid carcinoma. As IPNB, especially without visible mucin secretion, is considered to be a heterogeneous group of diseases, such an unexpected association could occur.

Keywords: Intraductal papillary neoplasm of the bile duct, intraductal papillary mucinous neoplasm, gastric type, colloid carcinoma

Introduction

Intraductal papillary neoplasm of the bile duct (IPNB) has been designated as a papillary growth of neoplastic epithelium with a fibrovas-cular core [1]. It exists in a pathway of multi-step-carcinogenesis toward cholangiocarcino-ma [2]. IPNB shares clinicopathological fea-tures with pancreatic intraductal papillary mucinous neoplasm (IPMN). Four subtypes of IPMN, pancreatobiliary type, intestinal type, gastric type, and oncocytic type, are also observed in IPNB in that order of frequency [1], and the intestinal type tends to be associated with colloid carcinoma in cases of an invasive component [3]. Colloid carcinoma developing in IPNB usually presents with macronodular growth in contrast to the periductal infiltrative growth of tubular adenocarcinoma [2].

IPNB includes tumors without macroscopically visible mucin secretion. IPNB with visible mucin secretion exhibits striking similarities to IPMN. On the other hand, IPNB without visible mucin

secretion harbors heterogeneous lesions: many of them more or less resembling usual cholan-giocarcinomas and others more similar to IPMN [4].

Herein, we present a case of an 82-year-old female patient with IPNB without visible mucin secretion, which developed in the intrapancre-atic common bile duct. It was classified as gas-tric-type IPMN and showed invasion as a form of colloid carcinoma. Gastric-type IPNB contain-ing an invasive component of colloid carcinoma is rare, and the number of well-described cases is limited [5].

Clinical findings

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showed the following elevated values: total bili-rubin 10.1 mg/dL, direct bilibili-rubin 7.9 mg/dL, aspartate transaminase 122 IU/L, alanine transaminase 153 IU/L, alkaline phosphatase 1280 IU/L, and gamma-glutamyl transpepti-dase 699 IU/L. The CA19-9 level was elevated to 390 U/mL (normal range: 0-37 U/mL). Abdominal contrast-enhanced computed tomo- graphy (CT) revealed a tumor in the intrapan-creatic common bile duct, measuring 20 × 18 mm (Figure 1A). The peripheral portion of the tumor was predominantly enhanced on the arterial phase compared with the plain CT image (Figure 1B, 1C). Magnetic resonance imaging (MRI) of the tumor showed low-signal

high-signal intensity on a T2-weighted image (Figure 1E). To obtain a definitive diagnosis, aspiration biopsy cytology was performed, and the diagnosis of adenocarcinoma was made. Subsequently, pancreatoduodenectomy was conducted. The patient’s postoperative course was complicated with pancreatic fistula; even-tually, the patient was discharged from the hos-pital on day 72 after the surgery with recovery to a healthy condition.

Pathological findings

[image:2.612.89.524.70.476.2]

The surgically resected specimen revealed a tumor, measuring 22 × 20 × 18 mm, in the

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Gastric-type IPNB progressing to colloid carcinoma

On the cut surface, it showed a mucinous appearance and it infiltrated into the wall of the bile duct with pushing borders (Figure 2B). Histopathologically, the tumor was composed of intraductal and invasive components. The intraductal component showed papillary growth with fibrovascular cores; the invasive compo-nent revealed abundant extracellular mucin (Figure 3A). The invasive component and the intraductal component showed gradual transi-tion with an intervening area of mucin-hyperse-creting dilated glands (Figure 3B). The intra-ductal component consisted of tumor cells con-taining abundant mucin in the cytoplasm and mildly enlarged nuclei with indistinct nucleoli (Figure 3C). The invasive component was com-posed of tumor cells present in the periphery of the mucinous lake or floating in the mucinous lake; their nuclei were moderately enlarged with prominent nucleoli (Figure 3D).

The antibodies used and the results of immu-nohistochemistry (IHC) are shown in Table 1. Tumor cells were entirely positive for CK7 (Figure 4A), and only the invasive component was weakly positive for CK20 (Figure 4B). All tumor cells were negative for CDX2 (Figure 4C). MUC1 immunostaining was observed in the secreted mucin but not in tumor cells (Figure 4D). MUC2 immunostaining was present only

focally in the intraductal component and dif-fusely in the invasive component (Figure 4E). MUC5AC displayed patchy positivity in the intra-ductal component and diffuse positivity in the invasive component (Figure 4F). Almost all the tumor cells were positive for MUC6 (Figure 4G). The Ki-67 labeling index was 4.6% in the intra-ductal component and 32% in the invasive component, based on counting 1000 cells (Figure 4H). Diffuse nuclear accumulation of p53 was observed in both components (Figure 4I).

The diagnosis of gastric-type IPNB (mucin-con-taining MUC6+ cells) associated with colloid carcinoma (abundant extracellular mucin with weakly CK20+ and MUC2+ tumor cells) was rendered.

A mutational analysis of KRAS codon 12 and 13 was performed. It did not reveal any muta-tion in these codons.

Discussion

IPNB accounts for 7% to 38% of all bile duct carcinomas [6-8]. It can develop at the biliary confluence (59%), in the distal common bile duct (31%), or within the liver (10%). As for inva-sion (seen in 74% of cases), IPNB in the distal bile duct (93%) shows the highest frequency,

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followed by IPNB in the hilus (65%); IPNB within the liver shows the lowest frequency (25%) [8]. Regarding the histopathological subtypes of the invasive component, Rocha et al. reported that 27 of 29 (93%) IPNB analyzed displayed tubular carcinoma; the other two cases were colloid carcinoma and minimally invasive carci-noma with mucinous features [8]. The survival of patients is related to the histopathological

[image:4.612.89.525.71.504.2]

subtype of the invasive component: those with mucinous carcinoma have a better prognosis than those with tubular adenocarcinoma [8]. The survival of patients with IPNB has been shown to be better than that of patients with conventional cholangiocarcinoma [6, 9, 10]. As mucinous carcinoma was identified in the inva-sive component in our case, the prognosis of the patient is expected to be relatively better.

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Gastric-type IPNB progressing to colloid carcinoma

In terms of IHC, CK20 is expressed in more than half of IPNBs; the intestinal type is associ-ated with CK20 expression, whereas the gas-tric type does not usually show CK20 expres-sion [3]. The MUC expresexpres-sion patterns are cor-related with the histopathological subtypes of IPNB [1, 3]. MUC1 expression is occasionally seen in the pancreaticobiliary type. MUC2 expression is observed in approximately half of IPNB cases; its expression is more often seen in the intestinal type than in the pancreatobili-ary, gastric, and oncocytic types. MUC5AC can be expressed in any of the four types, without being specific to any one type. MUC6 expres-sion is observed in the gastric type and onco-cytic type. In our case, MUC6 expression but no CK20 expression in the intraductal component was consistent with gastric-type IPNB, since it did not show oncocytic morphology.

IPNB includes tumors without macroscopically visible mucin secretion. Ohtsuka et al. reported that IPNB without macroscopically visible mucin secretion exhibits p53 positivity in half of the cases, while IPNB with visible mucin secretion does not show p53 positivity [4]. This finding is also true in our case, in which visible mucin secretion was not apparent and diffuse nuclear accumulation of p53 was observed. With regard to carcinogenesis, IPNB and biliary intraepithelial neoplasia (BilIN) have been pro-posed as 2 precursor lesions of invasive chol-angiocarcinoma [2]. IPNB and BilIN are sup-posed to be analogous to IPMN and pancreatic intraepithelial neoplasia (PanIN), respectively. Nuclear p53 accumulation is reported to be more frequently observed in PanIN-3, which

includes carcinoma in situ, than in carcinoma in situ arising in IPMN [11, 12]. Since IPNB with visible mucin secretion is less invasive than IPNB without visible secretion and shows p53 negativity on IHC, it is similar to IPMN, suggest-ing that IPNB with visible mucin secretion may follow a similar carcinogenic pathway to that of IPMN and that it would be the prototype of the IPNB carcinogenic pathway [4]. On the other hand, IPNB without visible mucin secretion is more invasive than IPNB with visible mucin secretion and has some propensity toward nuclear accumulation of p53 on IHC, which is more similar to PanIN than to IPMN. These find-ings indicate that some IPNBs without visible mucin secretion might originate via a similar pathway from BilIN to conventional cholangio-carcinoma [4]. However, in our case, the inva-sive component was colloid carcinoma with a MUC1-/MUC2+ expression pattern. Colloid car-cinoma arising from IPNB generally exhibits a MUC1-/MUC2+ expression pattern [2]. These facts suggest that the tumor in our case devel-oped through an IPNB carcinogenic pathway, since invasive carcinoma arising from BilIN con-sists of tubular adenocarcinoma showing a MUC1+/MUC2- expression pattern [2].

[image:5.612.91.527.83.230.2]

The genetic background of IPNB is not similar to that of IPMN [13]. GNAS codon 201 muta-tion, which is found in two thirds of IPMNs and is a more common mutation than KRAS [14, 15], is a less common mutation than KRAS in IPNB [16]. In addition, KRAS mutation, which was not detected in our case, is also less com-mon in IPNB than in IPMN [13]. The pathogen-esis of IPNB might be distinct from that of IPMN in spite of the morphological similarities. Large Table 1. Antibodies used in the present study

Antibody to Clone Dilution Pretreatment Source Immunohistochemical results Intraductal component Invasive component CK7 OV-TL 12/30 1:100 HIER Dako diffuse, strong diffuse, storng CK20 Ks20.8 1:100 HIER Dako negative patchy, weak CDX2 AMT28 1:50 HIER Novocastra negative negative MUC1 Ma695 1:100 HIER Novocastra negative negative MUC2 Ccp58 1:100 HIER Novocastra focal, weak diffuse, storng MUC5AC CLH2 1:50 HIER Novocastra patchy, weak to strong diffuse, storng MUC6 CLH5 1:50 HIER Novocastra diffuse, strong diffuse, storng Ki-67 MIB-1 1:100 HIER Dako 4.6% 32% p53 DO-7 1:100 HIER Dako diffuse, strong diffuse, storng

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[image:6.612.89.525.70.647.2]
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Gastric-type IPNB progressing to colloid carcinoma

and comprehensive sequencing studies are needed to clarify the genetic background of IPNB.

In conclusion, we report a rare case of gastric-type IPNB progressing to colloid carcinoma. As IPNB, especially IPNB without macroscopically visible mucin secretion, seems to be more diverse than IPMN, such an unexpected asso-ciation would be more likely to occur in cases of IPNB than in cases of IPMN.

Disclosure of conflict of interest

None.

Address correspondence to: Dr. Shogo Tajima, Department of Pathology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-Ku, Tokyo 113-0033 Japan. Tel: +81-3-5841-3341; Fax: +81-3-3815-8379; E-mail: stajima-tky@umin.ac.jp

References

[1] Nakanuma Y, Sato Y, Ojima H, Kanai Y, Aishima S, Yamamoto M, Ariizumi S, Furukawa T, Hayashi H, Unno M, Ohta T; Hepatolithiasis Subdivision of Intractable Hepatobiliary Diseases Study Group of Japan. Clinico- pathological characterization of so-called ‘‘cholangiocarcinoma with intraductal lary growth” with respect to “intraductal papil-lary neoplasm of bile duct (IPNB)”. Int J Clin Exp Pathol 2014; 7: 3112-3122.

[2] Nakanuma Y, Sasaki M, Sato Y, Ren X, Ikeda H and Harada K. Multistep carcinogenesis of perihilar cholangiocarcinoma arising in the in-trahepatic large bile ducts. World J Hepatol 2009; 1: 35-42.

[3] Nakanuma Y, Zen Y, Harada K, Ikeda H, Sato Y, Uehara T and Sasaki M. Tumorigenesis and phenotypic characteristics of mucin-producing bile duct tumors: an immunohistochemical ap-proach. J Hepatobiliary Pancreat Sci 2010; 17: 211-222.

[4] Ohtsuka M, Kimura F, Shimizu H, Yoshidome H, Kato A, Yoshitomi H, Furukawa K, Takeuchi D, Takayashiki T, Suda K, Takano S, Kondo Y and Miyazaki M. Similarities and differences be-tween intraductal papillary tumors of the bile duct with and without macroscopically visible mucin secretion. Am J Surg Pathol 2011; 35: 512-521.

[5] Kang GH, Moon HS, Lee ES, Kim SH, Sung JK, Lee B and Jeong HY. A case of colloid carcino-ma arising in association with intraductal

pap-illary neoplasm of the liver. Korean J Gastroenterol 2012; 60: 386-390.

[6] Yeh CN, Jan YY, Yeh TS, Hwang TL and Chen MF. Hepatic resection of the intraductal papil-lary type of peripheral cholangiocarcinoma. Ann Surg Oncol 2004; 11: 606-611.

[7] Barton JG, Barrett DA, Maricevich MA, Schnelldorfer T, Wood CM, Smyrk TC, Baron TH, Sarr MG, Donohue JH, Farnell MB, Kendrick ML, Nagorney DM, Reid Lombardo KM and Que FG. Intraductal papillary mucinous neo-plasm of the biliary tract: a real disease? HPB (Oxford) 2009; 11: 684-691.

[8] Rocha FG, Lee H, Katabi N, DeMatteo RP, Fong Y, D’Angelica MI, Allen PJ, Klimstra DS and Jarnagin WR. Intraductal papillary neoplasm of the bile duct: a biliary equivalent to intraductal papillary mucinous neoplasm of the pancre-as? Hepatology 2012; 56: 1352-1360. [9] Tajima Y, Kuroki T, Fukuda K, Tsuneoka N,

Furui J and Kanematsu T. An intraductal papil-lary component is associated with prolonged survival after hepatic resection for intrahepat-ic cholangiocarcinoma. Br J Surg 2004; 91: 99-104.

[10] Hoang MP, Murakata LA, Katabi N, Henson DE and Albores-Saavedra J. Invasive papillary car-cinomas of the extrahepatic bile ducts: a clini-copathologic and immunohistochemical study of 13 cases. Mod Pathol 2002; 15: 1251-1258.

[11] Abe K, Suda K, Arakawa A, Yamasaki S, Sonoue H, Mitani K and Nobukawa B. Different pat-terns of p16INK4A and p53 protein expres-sions in intraductal papillary-mucinous neo-plasms and pancreatic intraepithelial neopla-sia. Pancreas 2007; 34: 85-91.

[12] Moriya T, Kimura W, Semba S, Sakurai F, Hirai I, Ma J, Fuse A, Maeda K and Yamakawa M. Biological similarities and differences between pancreatic intraepithelial neoplasias and intra-ductal papillary mucinous neoplasms. Int J Gastrointest Cancer 2005; 35: 111-119. [13] Matthaei H, Wu J, Dal Molin M, Debeljak M,

Lingohr P, Katabi N, Klimstra DS, Adsay NV, Eshleman JR, Schulick RD, Kinzler KW, Vogelstein B, Hruban RH and Maitra A. GNAS codon 201 mutations are uncommon in intra-ductal papillary neoplasms of the bile duct. HPB (Oxford) 2012; 14: 677-683.

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[15] Furukawa T, Kuboki Y, Tanji E, Yoshida S, Hatori T, Yamamoto M, Shibata N, Shimizu K, Kamatani N and Shiratori K. Whole-exome se-quencing uncovers frequent GNAS mutations in intraductal papillary mucinous neoplasms of the pancreas. Sci Rep 2011; 1: 161.

Figure

Figure 1. Computed tomography (A-C) and magnetic resonance imaging (D, E) findings. (A) The tumor (arrow) was located in the intrapancreatic common bile duct, measuring 20 × 18 mm
Figure 3. Microscopic findings. (A) The tumor was composed of intraductal and invasive components
Table 1. Antibodies used in the present study
Figure 4. Immunohistochemical findings. A. Almost all the tumor cells were positive for CK7 (× 100)

References

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