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Changing Viral Tropism Using Immunoliposomes Alters the Stability of Gene Expression: Implications for Viral Vector Design

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Figure

Figure 1. Expression of surface receptors. HSVECs were cultured and the surface expres-80 ng/mL IFN-isotype-control antibody, and the dark black lines are specific antibodies againstsion of CD62E/P, CD71, and CD106 were analyzed using flow cytometry
Figure 2. Transducibility of ECs by retroviruses. pBullet-EGFP was used to generate am-
Table 1. Gene transfer with different pseudotyped MMLV, virosomes, andimmunovirosomes.
Figure 4. Early kinetics of gene expression. HSVECs that had been stimulated with 80 ng/mL TNF-was performed at 30, 60, 180, 360, 720, and 1440 min to determine the percentage of EGFP-positive cells (transfection efficiency) (A)and the mean fluorescence in
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