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Stem cell derived basal forebrain cholinergic neurons from Alzheimer’s disease patients are more susceptible to cell death

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Figure

Figure 1 Differentiation of iPSCs into ventral forebrain neural progenitors. (A) 72 hours of SHH treatment of iPSCs (Control 1 line)-derivedneural progenitors result in translocation of downstream effector Gli1 into the nucleus, Scale bar = 42 μm
Figure 2 Generation of basal forebrain cholinergic neurons from iPSCs. (A) Over 90% of cells derived from SHH/FGF8 treated neuralprogenitors (Control 1 line shown) are Map2 positive neurons
Figure 3 Control and AD iPSCs-derived BFCNs express functional voltage-gated sodium and calcium channels.traces evoked by step depolarization in the absenceBFCNs, note the elimination of the fast-inactivating inward current in the presence of TTX
Figure 4 AD-E3/E4 BFCNs have elevated Aβ42/Aβ40 ratios and altered responses to γ-secretase inhibitors
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