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Polyubiquitylation of AMF requires cooperation between the gp78 and TRIM25 ubiquitin ligases

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Figure

Figure 1: AMF binds the cytoplasmic domain of gp78. (A) Schematic representation of gp78
Figure 4: E4-like activity of gp78. In vitro ubiquitylation reactions were set up with purified proteins as indicated to measure polyubiquitylation of Ub-DHFR-his
Figure 5: gp78 is a substrate of TRIM25. (A) In vitro ubiquitylation of GST-gp78C by TRIM25
Figure 6: TRIM25 accelerates the metabolic degradation of gp78. (A) Degradation of gp78 is enhanced by overexpression of TRIM25

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