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Rescue of high specificity Cas9 variants using sgRNAs with matched 5’ nucleotides

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Figure

Fig. 1 High-specificity Cas9 variants with attenuated cleavage activity.Red trianglesG (A or C or T); D, not C (A or G or T).encoding Cas9-WT or variants andLocations of the alanine substitutions introduced in Cas9-WT to create eCas9-1.1 or Cas9-HF1 are sh
Fig. 2 Reduced editing activity of high-fidelity Cas9 variants at target sites with a non-G 5on-target sites with a 5frequencies (%) of the 26 target sites are described in Additional file 1: Table S1
Fig. 3 Recovery of editing efficiency of high-fidelity Cas9 variants using HH ribozyme-linked sgRNAs
Fig. 4 Specificities of high-fidelity Cas9 variants in combination with HH-X20 sgRNA. The HH-X20 sgRNA expression plasmid was co-transfectedwith the plasmid encoding Cas9-WT or a Cas9 variant into HeLa cells

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