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Targeted Mutagenesis of Beta Lactoglobulin Gene in Caprine Fetal Fibroblasts by Context Dependent Assembly Zinc Finger Nucleases

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Figure

Table 1. Zinc fingers used in ZFN (CoDA source)
Figure 1. Confirmation of ZFN-induced mutations by the Surveyor nuclease assay. 1. Amplicons from ZFN-treated cells, a 535-bp PCR product was cleaved into two frag-ments: 378 bp and 157 bp; 2
Figure 2. DNA sequencing revealed the mutation at ZFN targeted region. To detect mutations at ZFN targeted region in a single-cell colony, the PCR products of a single-cell colony were directly se-quenced

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