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Immunosuppressant FK506 induces interleukin 6 production through the activation of transcription factor nuclear factor (NF) kappa(B) Implications for FK506 nephropathy

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Figure 1. FK506 activates NF-mouse mesangial cells (�B in nonlymphoid cells. L-TK (A) or B) were treated for 30 min with IL-1 (21 ng/ml), LPS (10 �g/ml) or various concentrations of FK506 as indicated, and nuclear extracts were prepared and analyzed on EMS
Figure 3. FK506 induces IL-6 gene expression and IL-6 production in mesangial cells in vitro
Figure 5. Enhanced urinary IL-6 excretion after FK506 treatment. Mice were intramuscularly injected with 5 mg/kg per d of FK506 for 2, 4, 6, or 8 wk or with a FK506 vehicle alone (control) as described in Methods
Figure 7. Histopathological abnormalities in kidney of FK506-treated mice. Kidney tissue sections from control mice (A) or from mice treated with FK506 (5 mg/kg per d) for 8 wk (B and C) were stained with periodic acid-Schiff reagents (A and B) or hematoxy

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