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SIRT1 and LSD1 competitively regulate KU70 functions in DNA repair and mutation acquisition in cancer cells

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Academic year: 2020

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Figure 1: Stress-induced change of LSD1 and SIRT1 binding with KU70. A. Co-IP of endogenous KU70 with SIRT1 (upper panel) or LSD1 (lower panel) at baseline and two time-points (24 and 48 hours) following 2.5μM Imatinib (IM) administration in KCL-22 cells
Figure 2: Confocal imaging of SIRT1, LSD1 and KU70 in KCL-22 cells after DNA damage. Co-localization of KU70 with γH2AX A., SIRT1 B
Figure 3: Confocal imaging of SIRT1, LSD1 and KU70 in KU812 cells after DNA damage. Co-localization of KU70 with γH2AX A., SIRT1 B
Figure 4: Opposite recruitment of LSD1 and SIRT1 to the ABL locus. A, B. A, agarose gel images of standard ChIP-PCR for ABL exon 6 and β-actin in KCL-22 cells treated with 2.5μM IM for 0, 12, 24 or 48 hours
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