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The impact of impurities in various crude A annua extracts on the analysis of artemisinin by liquid chromatographic methods

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Figure

Fig. 1: Average (n=5) signal intensity of artemisinin standard (1000ÎĽg mL-1 in acetonitrile) as
Fig. 2: Combined HPLC-UV calibration curve of analytical runs undertaken over a period of
Fig. 3: Comparison of artemisinin standard (1000μg mL-1) HPLC-UV chromatograms at 192and 210nm when analysed using the Modified HPLC Protocol method; Mobile phaseacetonitrile-water-methanol (50:30:20, v/v/v), SunFire C18 250mm×4.6mm×5μm at column flow rate 1mL min-1 and column temperature 40°C.
Fig. 4: HPLC-UV analysis of crude A. annua extracts produced using ethyl acetate, hexane
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