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Endothelial pyruvate kinase M2 maintains vascular integrity

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Figure

Figure 1. ECs predominantly express PKM2 over PKM1. (A) Schematic illustration of alternative splicing of PKM1 and PKM2
Figure 2. PKM2 is crucial for EC proliferation and migration in vitro. (A) Western blot analysis of PKM2 and PKM1 protein expression in HUVECs demon-strates that the siRNAs used in this study give PKM2-specific and efficient knockdown
Figure 3. PKM2 is crucial for angiogenesis in vivo. (arrows) is analyzed by costaining of pH3 and CD31
Figure 4. Glycolysis is impaired in PKM2 knockdown ECs. (A) Extracellular acidification rate (ECAR) of HUVECs with si-control/scrambled RNA (siC) versus siPKM2 in the absence and presence of oligomycin (Oligo) demonstrating basal and maximal glycolytic rat
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