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Single molecule FRET analysis of DNA binding proteins

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Figure

Fig. 1 Schematic representation of an objective-based TIRF instrument. Individual fiber-coupled lasers are combined and coupled into a single excitation beam, which is expanded with a set of lenses (L)
Fig. 2 Schematic representation of sample immobilization. The glass coverslip is amine-modified, and the NHS-ester form of mPEG and biotin-PEG are covalently conjugated to the surface
Fig. 3 Schematic diagram of sample chambers for (a) single-molecule experiments and (b) fluorescent bead calibration

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