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Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production

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Figure

Fig. 1 Schematic illustration of the experimental setup. Cultivations are performed in multiple individual shake flasks in parallel
Fig. 2 Cultivation of filling volume. Cultivation conditions: 250 mL unbaffled shake flasks, Bacillus licheniformis DSM 8785 under oxygen-limited conditions
Fig. 3 Effects of buffering on 2,3-butanediol production with Bacillus licheniformis DSM 8785
Fig. 4 Glucose consumption and product formation during the flasks, temperature: 37 °C, shaking frequency: 350/100 rpm, shaking for acetoin
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