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Manipulating the sleeping beauty mutase operon for the production of 1-propanol in engineered Escherichia coli

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Academic year: 2020

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Figure 1 The genetically engineered central metabolic pathway under anaerobic conditions showing the activation of the Sbm operon(sbm, ygfD, and ygfG), and the expression of various adhEs used in this study
Figure 2 A schematic representation of the triple-plasmidexpression systems utilized for 1-propanol production
Table 1 1-Propanol and other metabolite titers (mg/L) in reduced M9 minimal media using E
Figure 4 End point secretion profile of major end productsfrom anaerobic fermentations of WT-adhE2(CA)3 at five opticaldensities (OD600), profiled by 1D-1H-NMR
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